盾叶薯蓣中皂甙的提取分离、结构剖析及生物活性研究
发布时间：2018-10-10 13:03
【摘要】： 研究背景 钉螺是日本血吸虫的唯一中间宿主。因此，控制钉螺是血吸虫病防治的关键技术之一。目前常用的氯硝柳胺等化学灭螺剂，虽有高效速效的技术优势，但存在对鱼毒性大及价格高等弊病，因而从上世纪30年代以来，研制灭螺高效、环境安全及价格低廉的植物灭螺剂成了国内外研究的热点。但至今尚未研究出一种达到WHO要求的植物灭螺剂。 研究的内容和意义 开展杀螺显效植物DZ根茎中皂甙的提取分离、结构剖析及生物活性研究，为创制WHO标准的新型杀螺药物提供设计依据和物质基础。 研究方法和结果 DZ中皂甙的提取分离和结构剖析研究：使用DZ根茎原粉，经80％EtOH超声水浴浸提，离心、活性炭脱色、结晶及CCL法的分离纯化，获得有效皂甙单体DZ-I。经HPLC-VWD和ELSD检测，DZ-I的纯度为98％，结构解析进行中。 DZ-I的生物活性研究：依照WHO灭螺试验法研究，8．0 mg·L~(-1)和16．0 mg·L~(-1)。的DZ-I浸泡48h，灭螺率分别为91．6％和100％。 使用钉螺软体组织制备的GPT，在经L_(25)(5~6)正交试验法确定GPT反应的最佳组合条件(酶浓度0．1mg·mL~(-1)，底物浓度5μmol·L~(-1)，反应体系pH6．5，反应温度35℃，反应时间30min)下，用1．0、5．0、10、50、100 mg·L~(-1)的DZ-I作用GPT，其诱导活性分别为168、138、96、75及57％，结果显示低剂量DZ-I的诱导活性高。 使用平板抑菌法，50 mg·L~(-1)对棉花枯萎菌、水稻纹枯菌、黄瓜灰霉菌、小麦赤霉菌、苹果轮纹菌及棉花炭疽菌48h的生长抑制率分别为26．32、10．26、17．39、3．70、28.30及4．55％
[Abstract]:Background Oncomelania hupensis is the only intermediate host of Schistosoma japonicum. Therefore, snail control is one of the key technologies for schistosomiasis control. The commonly used chemical snail killing agents such as niclosamide have the technical advantage of high efficiency and quick effect, but they have many disadvantages such as high toxicity to fish and high price, so since the 1930s, the snail killing efficiency has been developed. Environmental safety and low-cost plant molluscicides have become the focus of research at home and abroad. But up to now, no plant snail killer has been developed to meet the requirements of WHO. The content and significance of the study carried out the extraction, isolation, structure analysis and biological activity of saponins from the rootstock of DZ, which provided the design basis and material basis for the creation of new snail killing drugs of WHO standard. Methods and results the extraction, isolation and structure analysis of saponins from DZ: DZ rhizome raw powder was extracted by 80%EtOH ultrasonic water bath, centrifugation, activated carbon decolorization, crystallization and purification by CCL method. Obtaining effective saponin monomer DZ-I. The purity of DZ-I was 98% by HPLC-VWD and ELSD. The bioactivity of DZ-I was studied according to WHO method, 8.0 mg L ~ (-1) and 16.0 mg L ~ (-1). The snail killing rates were 91.6% and 100% respectively. The GPT, prepared by the soft tissue of Oncomelania hupensis was determined by L _ (25) (5 ~ 6) orthogonal test under the optimal combination conditions (enzyme concentration 0.1mg mL~ (-1), substrate concentration 5 渭 mol L ~ (-1), pH6.5, reaction temperature 35 鈩，
本文编号：2261873
[Abstract]:Background Oncomelania hupensis is the only intermediate host of Schistosoma japonicum. Therefore, snail control is one of the key technologies for schistosomiasis control. The commonly used chemical snail killing agents such as niclosamide have the technical advantage of high efficiency and quick effect, but they have many disadvantages such as high toxicity to fish and high price, so since the 1930s, the snail killing efficiency has been developed. Environmental safety and low-cost plant molluscicides have become the focus of research at home and abroad. But up to now, no plant snail killer has been developed to meet the requirements of WHO. The content and significance of the study carried out the extraction, isolation, structure analysis and biological activity of saponins from the rootstock of DZ, which provided the design basis and material basis for the creation of new snail killing drugs of WHO standard. Methods and results the extraction, isolation and structure analysis of saponins from DZ: DZ rhizome raw powder was extracted by 80%EtOH ultrasonic water bath, centrifugation, activated carbon decolorization, crystallization and purification by CCL method. Obtaining effective saponin monomer DZ-I. The purity of DZ-I was 98% by HPLC-VWD and ELSD. The bioactivity of DZ-I was studied according to WHO method, 8.0 mg L ~ (-1) and 16.0 mg L ~ (-1). The snail killing rates were 91.6% and 100% respectively. The GPT, prepared by the soft tissue of Oncomelania hupensis was determined by L _ (25) (5 ~ 6) orthogonal test under the optimal combination conditions (enzyme concentration 0.1mg mL~ (-1), substrate concentration 5 渭 mol L ~ (-1), pH6.5, reaction temperature 35 鈩，
本文编号：2261873
本文链接：https://www.wllwen.com/yixuelunwen/liuxingb/2261873.html
