邯郸市2010-2012年肠道病毒71型VP1基因特征分析

发布时间：2018-10-15 18:15

【摘要】：目的明确邯郸市2010-2012年EV71流行株的基因型及其VP1区基因特征,为手足口病防治提供分子生物学依据。方法用RD细胞分离培养EV71病毒,RT-PCR方法扩增VP1区基因片段,进行核苷酸和氨基酸序列同源性分析,构建系统进化树。结果邯郸市EV71分离株VP1区核苷酸同源性为96.4%~100.0%,与A、B基因型代表株VP1区核苷酸同源性在81.8%~82.7%与83.6%~84.5%之间。与已知基因型C1、C2、C3、C4代表株的核苷酸同源性分别在89.9%~90.3%、89.3%~90.1%、87.9%~88.7%和96.0%~96.9%之间,与1998年台湾分离株同源性为92.4%~93.2%,与2008年阜阳、2009年河南、2010年河北和2011年陕西分离的EV71代表株同源性在96.6%~99.1%之间。基于VP1区构建的EV71系统进化树显示,这14株EV71均为C4a亚型。结论邯郸市2010-2012年EV71流行株为C4a亚型,与中国大陆近年流行株分离结果一致。
[Abstract]:Objective to identify the genotypes and VP1 gene characteristics of EV71 epidemic strains from 2010 to 2012 in Handan City, and to provide molecular biological basis for the prevention and control of HFMD. Methods RD cells were used to isolate and culture EV71 virus. The VP1 region gene fragment was amplified by RT-PCR. The homology of nucleotide and amino acid sequence was analyzed and the phylogenetic tree was constructed. Results the nucleotide homology of the VP1 region of the EV71 isolates in Handan City was 96. 4%. The nucleotide homology of the VP1 region was 81.8% and 84.5% respectively with the VP1 region, which was the representative of AZB genotype. The nucleotide homology with the known genotype C _ 1C _ 2C _ 3C _ 3C _ 4 was 89.9, respectively. The nucleotide homology was 87.9% and 96.0%, respectively. The homology with the Taiwan isolate in 1998 was 92.493. 2%, and the homology with the representative strains of EV71 isolated from Fuyang, Henan, Hebei and Shaanxi in 2010 and 2011 was 96.6% and 99.1% respectively, and the homology was 92.40.92% with that of the Taiwan isolate in 1998, and the homology was 99.1% with the representative strains isolated from Fuyang in 2008, Henan Province in 2009, Hebei Province in 2010 and Shaanxi in 2011. The phylogenetic tree of EV71 based on VP1 region showed that the 14 EV71 strains were all C4a subtypes. Conclusion the epidemic strain of EV71 in Handan City from 2010 to 2012 is C4a subtype, which is consistent with the results of isolation of epidemic strains in mainland China in recent years.
【作者单位】：邯郸市疾病预防控制中心;
【分类号】：R373.2;R181.3

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