内蒙古地区乙型病毒性肝炎血清流行病学调查及乙肝病毒基因型研究
发布时间：2018-11-04 18:12
【摘要】： 目的为了了解内蒙古不同地区、不同人群中乙肝的流行状况,特别是实施大规模乙肝疫苗接种后的效果,开展此次全区范围的乙肝血清学调查。通过本实验可以初步了解内蒙古地区的乙肝病毒(HBV)基因型分布特点,为推测乙肝的流行趋势提供数据资料。方法在内蒙古地区12个盟市,采用多阶段抽样方法,在1-61岁常住人口选取1～6岁、7～16岁、17-61岁三个年龄组,共采集血清样本4021份。统一用ELISA方法检测乙肝表面抗原(HBsAg)、乙肝病毒表面抗体(HBsAb)、乙肝病毒核心抗体(HBcAb),对HBsAg阳性者再进行乙肝病毒e抗原(HBeAg)和乙肝病毒e抗体(HBeAb)检测。并对46份乙肝大三阳血样进行HBV DNA提取,用PCR实验扩增提取到的HBV DNA S区,根据PCR产物基因测序结果对样本HBV进行基因分型。结果内蒙古地区HBsAg人群总体阳性率2.71%,比1992年调查结果(6.35%)有明显降低。HBV人群总体感染率53.64%,比1992年调查结果(37.16%)有明显增高。本实验共46份样本血清的PCR体系测序成功。其中B型1例(2.17%),C型43例(93.48%),D型2例(4.35%)未发现其它HBV的基因型。结论内蒙古地区不同地区人群之间的HBsAg阳性率和HBV感染率差异有统计学意义(P＜0.01)。我区农村是我区乙肝高流行地区。针灸、创伤美容这两个因素为乙肝感染的危险因素。1992年前后出生的两个人群的HBsAg感染率之间差异有统计学意义(P＜0.01),1992年前后出生的两个人群的HBsAb感染率之间差异有统计学意义(P＜0.01),这可以说明1992年内蒙古地区乙肝疫苗纳入儿童计划免疫管理实施效果明显。2002年前后出生的两个人群的HBsAg感染率之间差异有统计学意义(P＜0.01),2002年前后出生的两个人群的HBsAb感染率之间差异有统计学意义(P＜0.01),这可以说明2002年将乙肝疫苗纳入儿童计划免疫及GAVI项目实施效果明显。内蒙古地区汉族人群中HBV的感染以C基因型HBV为主,B、D基因型HBV各占很少比例,未发现其他基因型HBV。既本实验共46份样本血清的PCR体系测序成功:B型1例(2.17%),C型43例(93.48%),D型2例(4.35%)。未发现其他基因型。
[Abstract]:Objective to investigate the prevalence of hepatitis B in different areas and populations in Inner Mongolia, especially the effect of mass hepatitis B vaccination, and to carry out a serological survey of hepatitis B in the whole region. The distribution of hepatitis B virus (HBV) genotypes in Inner Mongolia can be preliminarily understood by this experiment, and the data can be provided for the prediction of hepatitis B epidemic trend. Methods A total of 4021 serum samples were collected from 12 cities in Inner Mongolia. 4021 serum samples were collected from the resident population aged 1-61 years in three age groups, I. e., 1 or 6 years old, 7 ~ 16 years old and 17 to 61 years old. Hepatitis B surface antigen (HBsAg),) hepatitis B virus surface antibody (HBsAb),) hepatitis B virus core antibody (HBcAb),) was detected by ELISA method in HBsAg positive patients and then hepatitis B virus e antigen (HBeAg) and hepatitis B virus e antibody (HBeAb) were detected in HBsAg positive patients. HBV DNA was extracted from 46 samples of hepatitis B positive blood samples, and the extracted HBV DNA S region was amplified by PCR experiment. According to the results of PCR product gene sequencing, the sample HBV was genotyped. Results the overall positive rate of HBsAg in Inner Mongolia was 2.71, which was significantly lower than that in 1992 (6.35%). The total infection rate of HBV was 53.64, which was significantly higher than that of 1992 (37.16%). The PCR system of 46 serum samples was sequenced successfully. One case of type B (2.17% of), C type 43 cases (93.48%) of), D type 2 cases (4.35%) did not find other HBV genotypes. Conclusion there were significant differences in HBsAg positive rate and HBV infection rate among different population in Inner Mongolia (P < 0. 01). My district countryside is my district hepatitis B high epidemic area. Acupuncture, trauma and beauty were the risk factors of hepatitis B infection. There was a significant difference in HBsAg infection rate between the two groups born before and after 1992 (P < 0. 01). There was a significant difference in HBsAb infection rate between the two populations born around 1992 (P < 0. 01). It can be concluded that the effect of applying hepatitis B vaccine into children's immunization management in Inner Mongolia in 1992 is obvious, and the difference of HBsAg infection rate between the two groups born around 2002 is statistically significant (P < 0. 01). The difference of HBsAb infection rate between the two populations before and after 2002 was statistically significant (P < 0. 01), which indicated that the hepatitis B vaccine was introduced into the children's immunization program and the implementation of GAVI project was effective in 2002. In Inner Mongolia Han population, genotype C HBV was the main type of HBV infection, while genotype B D HBV accounted for a small proportion. No other genotypes of HBV. were found. The PCR system of 46 serum samples was sequenced successfully: type B (2.17%), C 43 cases, 93.48%), D 2 cases (4.35%). No other genotypes were found.
【学位授予单位】：内蒙古医学院
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R512.62;R181.3
本文编号：2310732
[Abstract]:Objective to investigate the prevalence of hepatitis B in different areas and populations in Inner Mongolia, especially the effect of mass hepatitis B vaccination, and to carry out a serological survey of hepatitis B in the whole region. The distribution of hepatitis B virus (HBV) genotypes in Inner Mongolia can be preliminarily understood by this experiment, and the data can be provided for the prediction of hepatitis B epidemic trend. Methods A total of 4021 serum samples were collected from 12 cities in Inner Mongolia. 4021 serum samples were collected from the resident population aged 1-61 years in three age groups, I. e., 1 or 6 years old, 7 ~ 16 years old and 17 to 61 years old. Hepatitis B surface antigen (HBsAg),) hepatitis B virus surface antibody (HBsAb),) hepatitis B virus core antibody (HBcAb),) was detected by ELISA method in HBsAg positive patients and then hepatitis B virus e antigen (HBeAg) and hepatitis B virus e antibody (HBeAb) were detected in HBsAg positive patients. HBV DNA was extracted from 46 samples of hepatitis B positive blood samples, and the extracted HBV DNA S region was amplified by PCR experiment. According to the results of PCR product gene sequencing, the sample HBV was genotyped. Results the overall positive rate of HBsAg in Inner Mongolia was 2.71, which was significantly lower than that in 1992 (6.35%). The total infection rate of HBV was 53.64, which was significantly higher than that of 1992 (37.16%). The PCR system of 46 serum samples was sequenced successfully. One case of type B (2.17% of), C type 43 cases (93.48%) of), D type 2 cases (4.35%) did not find other HBV genotypes. Conclusion there were significant differences in HBsAg positive rate and HBV infection rate among different population in Inner Mongolia (P < 0. 01). My district countryside is my district hepatitis B high epidemic area. Acupuncture, trauma and beauty were the risk factors of hepatitis B infection. There was a significant difference in HBsAg infection rate between the two groups born before and after 1992 (P < 0. 01). There was a significant difference in HBsAb infection rate between the two populations born around 1992 (P < 0. 01). It can be concluded that the effect of applying hepatitis B vaccine into children's immunization management in Inner Mongolia in 1992 is obvious, and the difference of HBsAg infection rate between the two groups born around 2002 is statistically significant (P < 0. 01). The difference of HBsAb infection rate between the two populations before and after 2002 was statistically significant (P < 0. 01), which indicated that the hepatitis B vaccine was introduced into the children's immunization program and the implementation of GAVI project was effective in 2002. In Inner Mongolia Han population, genotype C HBV was the main type of HBV infection, while genotype B D HBV accounted for a small proportion. No other genotypes of HBV. were found. The PCR system of 46 serum samples was sequenced successfully: type B (2.17%), C 43 cases, 93.48%), D 2 cases (4.35%). No other genotypes were found.
【学位授予单位】：内蒙古医学院
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R512.62;R181.3
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