北京地区肾综合征出血热分子流行病学调查研究
发布时间：2018-11-08 08:26
【摘要】： 本研究针对近年来北京地区肾综合征出血热(HFRS)发病日趋上升的疫情形势，就宿主动物、毒株特点及HFRS病人感染汉坦病毒(HV)的特征等方面进行了系统的分子流行病学调查研究。 首先选取近年来发病较高的海淀区城乡结合部作为调查点，捕捉宿主动物，运用现代分子生物学检测手段，基本确定这一地区优势宿主为褐家鼠，主要流行株是与S3亚型代表株Z37亲缘关系较近的的Seoul病毒。 然后从褐家鼠肺组织中分离了汉坦病毒，并对其中的BjHD01株M和S全基因序列进行了测定和分析。结果显示，它与其他Seoul病毒的基因序列基本相似，但其氨基酸序列具有独有特征。系统发育分析提示，北京地区毒株在其进化史上可能与不同来源的Seoul病毒存在广泛的交互作用。 通过病例感染病毒的分子流行病学调查研究提示在北京地区治疗的病人包括两部分：于外地感染在京治疗者及本地感染治疗者。北京地区Seoul病毒流行株可分为两个基因簇，一簇分布于北京西南、西北近郊和城市中心地区，另一簇分布于北京东北近郊和西北远郊地区，两簇在朝阳和顺义的交界区附近交叉分布。 北京市不同发病状况的地区流行毒株各有其独有的氨基酸特征。小家鼠虽然能够感染Seoul病毒，，但由于其体内病毒发生的变异，作为传播宿主的地位可能有限。无论是在动物宿主体内还是在病人体内，Seoul病毒均以变异混合体(Quasispcies)形式生存，其S片段3’末端均可发生末端序列缺失，这可能是病毒适应环境的机制之一。但缺失碱基数在人体和动物宿主体内可能不同，还有待于进一步研究。 本研究针对北京地区疫情形势从宿主、病原、病人三个角度、不同层面进行了系统的分子流行病学调查研究，对北京地区汉坦病毒的基因特征、感染来源、分布和流行规律进行了全面分析，深入探讨了北京近年来HFRS疫情攀升及病例分布不均衡的主要原因及病毒的适应机制、变异特点，为制定科学、有效的预防和控制措施提供了理论基础和决策依据。
[Abstract]:This study aimed at the increasing epidemic situation of hemorrhagic fever with renal syndrome (HFRS) in Beijing area in recent years. The characteristics of strain and the characteristics of Hantavirus (HV) infection in HFRS patients were investigated systematically by molecular epidemiology. Firstly, the urban and rural areas with high incidence in recent years were selected as the investigation points to capture host animals and to determine the dominant host of Rattus norvegicus basically by means of modern molecular biological detection. The main epidemic strain is Seoul virus, which is closely related to the representative strain Z37 of S3 subtype. Then Hantavirus was isolated from the lung tissue of Rattus norvegicus and the M and S gene sequences of BjHD01 strain were determined and analyzed. The results showed that its amino acid sequence was similar to that of other Seoul viruses, but its amino acid sequence had unique characteristics. Phylogenetic analysis suggested that the strains in Beijing might interact with Seoul viruses from different sources in their evolutionary history. The molecular epidemiologic investigation of cases of viral infection indicated that the patients treated in Beijing included two parts: those who had been treated in Beijing and those who had been treated in Beijing. The Seoul virus epidemic strains in Beijing can be divided into two clusters, one is in southwest Beijing, the other is in northwest suburb and urban center area, the other is located in northeast suburb and northwest suburb of Beijing. The two clusters are intersected near the junction of Chaoyang and Shunyi. The epidemic strains in different areas of Beijing have their unique amino acid characteristics. Although mice can infect Seoul virus, their status as a transmission host may be limited due to the variation of the virus in their bodies. Both in animal host and in patient, Seoul virus survives in the form of variant (Quasispcies), and its S fragment 3 'end can cause terminal deletion, which may be one of the mechanisms of virus adaptation to the environment. However, the number of missing bases may be different in human and animal hosts, which needs further study. In this study, a systematic molecular epidemiological investigation on the epidemic situation in Beijing was carried out from three aspects: host, pathogen and patient. The genetic characteristics and source of Hantavirus infection in Beijing were studied. The distribution and epidemic law were comprehensively analyzed, and the main causes of the rising HFRS epidemic situation and uneven distribution of cases in Beijing in recent years, as well as the adaptive mechanism and variation characteristics of the virus, were discussed in depth. Effective prevention and control measures provide the theoretical basis and decision-making basis.
【学位授予单位】：中国人民解放军军事医学科学院
【学位级别】：博士
【学位授予年份】：2007
【分类号】：R512.8;R181.3
本文编号：2317922
[Abstract]:This study aimed at the increasing epidemic situation of hemorrhagic fever with renal syndrome (HFRS) in Beijing area in recent years. The characteristics of strain and the characteristics of Hantavirus (HV) infection in HFRS patients were investigated systematically by molecular epidemiology. Firstly, the urban and rural areas with high incidence in recent years were selected as the investigation points to capture host animals and to determine the dominant host of Rattus norvegicus basically by means of modern molecular biological detection. The main epidemic strain is Seoul virus, which is closely related to the representative strain Z37 of S3 subtype. Then Hantavirus was isolated from the lung tissue of Rattus norvegicus and the M and S gene sequences of BjHD01 strain were determined and analyzed. The results showed that its amino acid sequence was similar to that of other Seoul viruses, but its amino acid sequence had unique characteristics. Phylogenetic analysis suggested that the strains in Beijing might interact with Seoul viruses from different sources in their evolutionary history. The molecular epidemiologic investigation of cases of viral infection indicated that the patients treated in Beijing included two parts: those who had been treated in Beijing and those who had been treated in Beijing. The Seoul virus epidemic strains in Beijing can be divided into two clusters, one is in southwest Beijing, the other is in northwest suburb and urban center area, the other is located in northeast suburb and northwest suburb of Beijing. The two clusters are intersected near the junction of Chaoyang and Shunyi. The epidemic strains in different areas of Beijing have their unique amino acid characteristics. Although mice can infect Seoul virus, their status as a transmission host may be limited due to the variation of the virus in their bodies. Both in animal host and in patient, Seoul virus survives in the form of variant (Quasispcies), and its S fragment 3 'end can cause terminal deletion, which may be one of the mechanisms of virus adaptation to the environment. However, the number of missing bases may be different in human and animal hosts, which needs further study. In this study, a systematic molecular epidemiological investigation on the epidemic situation in Beijing was carried out from three aspects: host, pathogen and patient. The genetic characteristics and source of Hantavirus infection in Beijing were studied. The distribution and epidemic law were comprehensively analyzed, and the main causes of the rising HFRS epidemic situation and uneven distribution of cases in Beijing in recent years, as well as the adaptive mechanism and variation characteristics of the virus, were discussed in depth. Effective prevention and control measures provide the theoretical basis and decision-making basis.
【学位授予单位】：中国人民解放军军事医学科学院
【学位级别】：博士
【学位授予年份】：2007
【分类号】：R512.8;R181.3
【引证文献】
相关硕士学位论文 前1条
1 刘勇;以灭活纯化肾综合征出血热疫苗为主的干预模式的流行病学效果评价[D];山东大学;2012年
本文编号：2317922
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