错配修复系统MLH1基因rs1800734多态性与肝细胞癌的关联研究
发布时间：2018-12-12 12:39
【摘要】：目的研究错配修复系统MLH1基因rs1800734多态性与肝细胞癌遗传易感性的关系。方法采用以医院为基础的病例对照研究方法,运用荧光定量聚合酶链反应(polymerase chain reaction,PCR)和非条件Logistic回归分析MLH1基因型在两组中分布频率的差异,以及基因多态性和环境因素的交互作用。结果病例组MLH1基因位点AA、AG和GG各基因型频率分别为37.09%、48.57%和14.35%,对照组中基因型频率分别为32.89%、47.46%和19.65%,差异无统计学意义(P=0.085)。多因素Logistic回归分析表明,与AA基因型相比,AG或者GG基因型的个体罹患HCC的风险OR值分别为1.102和1.544。病例组等位基因(A)频率(61.37%)较对照组(56.62%)增高(醊2=4.22,P=0.040),A等位基因携带者患肝细胞癌的危险性是G等位基因携带者的1.217倍。交互作用分析结果表明MLH1基因多态性与肿瘤家族史、乙型肝炎病毒表面抗原(epatitis B surface antigen,HBsAg)阳性之间均存在交互作用(P0.001),OR值分别为4.763和16.967。结论 MLH1基因可能是广西人群肝细胞癌的危险因素之一,其多态性与肿瘤家族史、HBsAg阳性之间在肝细胞癌发生中存在交互作用,能增加罹患肝细胞癌的风险。
[Abstract]:Objective to study the relationship between rs1800734 polymorphism of MLH1 gene and susceptibility to hepatocellular carcinoma (HCC). Methods A hospital-based case-control study was conducted. The frequency of MLH1 genotypes in the two groups was analyzed by fluorescence quantitative polymerase chain reaction (polymerase chain reaction,PCR) and non-conditional Logistic regression analysis. And the interaction between genetic polymorphism and environmental factors. Results the frequencies of AA,AG and GG genotypes in MLH1 gene locus were 37.09% and 14.35%, respectively. In the control group, the frequencies of AA,AG and GG genotypes were 32.89% and 19.65%, respectively. The difference was not statistically significant (P0. 085). Multivariate Logistic regression analysis showed that the OR values of the individuals with AG or GG genotype for HCC were 1.102 and 1.544, respectively, compared with the AA genotype. The allele (A) frequency (61.37%) in the case group was 1.217 times higher than that in the control group (56.62%). The results of interaction analysis showed that there was interaction between MLH1 gene polymorphisms and tumor family history, hepatitis B virus surface antigen (epatitis B surface antigen,HBsAg) positive (P0. 001), OR values were 4.763 and 16.967 respectively. Conclusion MLH1 gene may be one of the risk factors of hepatocellular carcinoma in Guangxi population. There is interaction between polymorphism and family history and HBsAg positive in the occurrence of hepatocellular carcinoma, which can increase the risk of hepatocellular carcinoma.
【作者单位】： 桂林医学院公共卫生学院流行病与卫生统计学教研室;广西医科大学公共卫生学院流行病与卫生统计学教研室;
【基金】：国家自然科学青年基金(81001285) 国家自然科学地区基金(81160359) 广西教育厅项目(200911MS162)
【分类号】：R735.7;R181.1
[Abstract]:Objective to study the relationship between rs1800734 polymorphism of MLH1 gene and susceptibility to hepatocellular carcinoma (HCC). Methods A hospital-based case-control study was conducted. The frequency of MLH1 genotypes in the two groups was analyzed by fluorescence quantitative polymerase chain reaction (polymerase chain reaction,PCR) and non-conditional Logistic regression analysis. And the interaction between genetic polymorphism and environmental factors. Results the frequencies of AA,AG and GG genotypes in MLH1 gene locus were 37.09% and 14.35%, respectively. In the control group, the frequencies of AA,AG and GG genotypes were 32.89% and 19.65%, respectively. The difference was not statistically significant (P0. 085). Multivariate Logistic regression analysis showed that the OR values of the individuals with AG or GG genotype for HCC were 1.102 and 1.544, respectively, compared with the AA genotype. The allele (A) frequency (61.37%) in the case group was 1.217 times higher than that in the control group (56.62%). The results of interaction analysis showed that there was interaction between MLH1 gene polymorphisms and tumor family history, hepatitis B virus surface antigen (epatitis B surface antigen,HBsAg) positive (P0. 001), OR values were 4.763 and 16.967 respectively. Conclusion MLH1 gene may be one of the risk factors of hepatocellular carcinoma in Guangxi population. There is interaction between polymorphism and family history and HBsAg positive in the occurrence of hepatocellular carcinoma, which can increase the risk of hepatocellular carcinoma.
【作者单位】： 桂林医学院公共卫生学院流行病与卫生统计学教研室;广西医科大学公共卫生学院流行病与卫生统计学教研室;
【基金】：国家自然科学青年基金(81001285) 国家自然科学地区基金(81160359) 广西教育厅项目(200911MS162)
【分类号】：R735.7;R181.1
【参考文献】
相关期刊论文 前4条
1 ;Synergistic Action of Clonorchiasis,HBV Infection and Alcohol Consumption on Primary Hepatocellular Carcinoma[J];Clinical Oncology and Cancer Research;2009年02期
2 曾小云;余红平;仇小强;纪龙;李龙Z，
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