一种强化戊二醛杀灭微生物效果与安全性的研究
发布时间：2019-01-30 11:48
【摘要】： 戊二醛被公认为一种广谱、高效、低刺激、低腐蚀、安全低毒、稳定的消毒剂,作为一种冷消毒灭菌剂,被广泛用于怕高温、怕腐蚀的医疗器械的首选消毒剂。近年来,戊二醛类消毒剂的生产厂家逐年增加,剂型种类繁多,产品质量参差不齐。我们在实际工作中发现,单纯根据产品使用说明书和《消毒技术规范》要求来配制和使用,无法最大限度地利用戊二醛的杀灭效果,不能完全反映戊二醛消毒剂的特点,不利于指导应用单位合理有效地使用戊二醛消毒剂。因此,在实验室对一种强化戊二醛——洁亿牌戊二醛消毒液进行了杀菌效果的系统分析,通过不同配方、不同影响因素下戊二醛含量、杀菌效果变化规律,从多方面评价该消毒剂的质量,探讨如何提高戊二醛消毒剂的杀菌效果,指导其实际应用;同时也借此工作为戊二醛消毒剂配方的研究提供实验室依据。 为了解洁亿牌戊二醛消毒液的杀菌性能、稳定性及其影响因素,我们在实验室对其进行了戊二醛含量测定、悬液定量杀灭试验、载体定性杀灭试验、杀菌影响因素测定、加速稳定性试验、能量试验;并通过模拟现场消毒灭菌试验、连续使用稳定试验等验证了该消毒剂的实用消毒效果;通过金属腐蚀性试验、急性经口毒性试验、一次完整皮肤刺激试验,了解该消毒剂对金属的破坏性及对人的急性毒性。 研究结果如下: 1.洁亿牌戊二醛消毒液为三元包装,A剂为添加有0.25%聚氧乙烯脂肪醇醚的戊二醛,B剂为防锈剂亚硝酸钠,C剂为缓冲剂碳酸氢钠。使用时取5g/L的B剂和0.4g/L的C剂加入戊二醛溶液中。防锈剂亚硝酸钠的添加会降低戊二醛的含量。在不同pH值下戊二醛含量的测定值有差异,随着pH的升高而降低。 2.洁亿牌戊二醛消毒液属稳定类消毒剂,具有高效、快速的杀菌特点。19200mg/L洁亿牌戊二醛消毒液对细菌芽胞作用2h;480mg/L对细菌繁殖体作用10min、960mg/L作用5min或1920mg/L作用2min,杀灭对数值均≥5.00;1920mg/L洁亿牌戊二醛消毒液对白色念珠菌作用15min,其杀灭对数值≥4.00,达消毒合格要求。 3.洁亿牌戊二醛消毒液可用于多次消毒污秽物品,其实用参考剂量为600mg/L。
[Abstract]:Glutaraldehyde is recognized as a kind of disinfectant with broad spectrum, high efficiency, low irritation, low corrosion, safety, low toxicity and stability. As a cold sterilizing agent, glutaraldehyde is widely used as the preferred disinfectant for medical devices afraid of high temperature and corrosion. In recent years, the manufacturers of glutaraldehyde disinfectants have increased year by year. In our practical work, we found that the preparation and use of glutaraldehyde could not be used to the maximum extent and could not reflect the characteristics of glutaraldehyde disinfectant solely according to the instructions for the use of the product and the requirements of the Technical Specification for Disinfection. It is unfavorable to guide the application units to use glutaraldehyde disinfectant reasonably and effectively. Therefore, the bactericidal effect of a kind of Glutaraldehyde fortified glutaraldehyde disinfectant solution was systematically analyzed in the laboratory. The content of glutaraldehyde and the change of germicidal effect were determined by different formulations and different influencing factors. The quality of the disinfectant was evaluated from many aspects, and how to improve the germicidal effect of glutaraldehyde disinfectant was discussed to guide its practical application. It also provides laboratory basis for the formulation of glutaraldehyde disinfectant. In order to understand the germicidal performance, stability and influencing factors of Jie-yi brand glutaraldehyde disinfectant solution, we carried out the determination of glutaraldehyde content, suspension quantitative killing test, carrier qualitative killing test and germicidal influence factor in laboratory. Accelerated stability test, energy test; The practical disinfection effect of the disinfectant was verified by simulating field disinfection sterilization test and continuous use stability test. Through metal corrosion test, acute oral toxicity test and a complete skin irritation test, it is understood that the disinfectant is destructive to metals and acute toxicity to human. The results are as follows: 1. Jieyi brand glutaraldehyde disinfectant was ternary packed, A was glutaraldehyde with 0.25% polyoxyethylene aliphatic alcohol ether, B was rust inhibitor sodium nitrite, and C was buffer sodium bicarbonate. When used, 5g/L B and 0.4g/L C were added to glutaraldehyde solution. The addition of sodium nitrite will reduce the content of glutaraldehyde. There were differences in glutaraldehyde content at different pH values, which decreased with the increase of pH. 2. Jieyi brand glutaraldehyde disinfectant is a stable disinfectant with high efficiency and fast bactericidal characteristics. 19200mg/L Jieyi brand glutaraldehyde disinfection solution acts on bacterial spores for 2 h. The killing logarithm of 480mg/L on bacterial propagator was more than 5.00 when 5min or 1920mg/L were exposed to 5min or 1920mg/L for 2 min. When 1920mg/L Jiayi brand glutaraldehyde disinfectant was exposed to Candida albicans for 15 min, the logarithmic value of killing was greater than 4.00, which reached the standard of disinfection. 3. Jieyi brand glutaraldehyde disinfectant solution can be used to disinfect contaminated articles for many times, and its practical reference dose is 600 mg / L.
【学位授予单位】：第三军医大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R187
本文编号：2418097
[Abstract]:Glutaraldehyde is recognized as a kind of disinfectant with broad spectrum, high efficiency, low irritation, low corrosion, safety, low toxicity and stability. As a cold sterilizing agent, glutaraldehyde is widely used as the preferred disinfectant for medical devices afraid of high temperature and corrosion. In recent years, the manufacturers of glutaraldehyde disinfectants have increased year by year. In our practical work, we found that the preparation and use of glutaraldehyde could not be used to the maximum extent and could not reflect the characteristics of glutaraldehyde disinfectant solely according to the instructions for the use of the product and the requirements of the Technical Specification for Disinfection. It is unfavorable to guide the application units to use glutaraldehyde disinfectant reasonably and effectively. Therefore, the bactericidal effect of a kind of Glutaraldehyde fortified glutaraldehyde disinfectant solution was systematically analyzed in the laboratory. The content of glutaraldehyde and the change of germicidal effect were determined by different formulations and different influencing factors. The quality of the disinfectant was evaluated from many aspects, and how to improve the germicidal effect of glutaraldehyde disinfectant was discussed to guide its practical application. It also provides laboratory basis for the formulation of glutaraldehyde disinfectant. In order to understand the germicidal performance, stability and influencing factors of Jie-yi brand glutaraldehyde disinfectant solution, we carried out the determination of glutaraldehyde content, suspension quantitative killing test, carrier qualitative killing test and germicidal influence factor in laboratory. Accelerated stability test, energy test; The practical disinfection effect of the disinfectant was verified by simulating field disinfection sterilization test and continuous use stability test. Through metal corrosion test, acute oral toxicity test and a complete skin irritation test, it is understood that the disinfectant is destructive to metals and acute toxicity to human. The results are as follows: 1. Jieyi brand glutaraldehyde disinfectant was ternary packed, A was glutaraldehyde with 0.25% polyoxyethylene aliphatic alcohol ether, B was rust inhibitor sodium nitrite, and C was buffer sodium bicarbonate. When used, 5g/L B and 0.4g/L C were added to glutaraldehyde solution. The addition of sodium nitrite will reduce the content of glutaraldehyde. There were differences in glutaraldehyde content at different pH values, which decreased with the increase of pH. 2. Jieyi brand glutaraldehyde disinfectant is a stable disinfectant with high efficiency and fast bactericidal characteristics. 19200mg/L Jieyi brand glutaraldehyde disinfection solution acts on bacterial spores for 2 h. The killing logarithm of 480mg/L on bacterial propagator was more than 5.00 when 5min or 1920mg/L were exposed to 5min or 1920mg/L for 2 min. When 1920mg/L Jiayi brand glutaraldehyde disinfectant was exposed to Candida albicans for 15 min, the logarithmic value of killing was greater than 4.00, which reached the standard of disinfection. 3. Jieyi brand glutaraldehyde disinfectant solution can be used to disinfect contaminated articles for many times, and its practical reference dose is 600 mg / L.
【学位授予单位】：第三军医大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R187
【引证文献】
相关硕士学位论文 前2条
1 赵月;鸡场土壤中大肠杆菌的检测与化学消毒剂的筛选[D];华中农业大学;2011年
2 张静;微生物法降解戊二醛的研究[D];成都理工大学;2013年
，本文编号：2418097
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