湖北钉螺血淋巴细胞的形态及其分类的研究

发布时间：2019-04-19 19:44

【摘要】：分别提取自湖南岳阳市君山区和四川西昌市普格县采集的湖北钉螺(Oncomelania hupensis)的血淋巴细胞,经吉氏染色后观察其形态,鉴定和计数其细胞类型,测量不同类型血淋巴细胞的形态特征。结果显示,根据细胞酸碱性和胞内颗粒性质可将钉螺血淋巴细胞分为5类:嗜酸性大透明细胞、嗜酸性小透明细胞、嗜碱性透明细胞、嗜碱性小颗粒细胞和嗜碱性大颗粒细胞。在5类细胞中,嗜酸性小透明细胞占细胞总数比例最大,在君山钉螺中为36.7%(229/624),在普格钉螺中为31.7%(257/810);其次是嗜碱性透明细胞,在君山钉螺中为23.1%(144/624),在普格钉螺中为24.4%(198/810);嗜碱性大颗粒细胞所占比例最小,分别为9.3%(58/624)和11.6%(94/810)。形态学上,嗜酸性大透明细胞长径最大、核质比最小,嗜酸性小透明细胞次之,碱性细胞长径较小,核质比较大。两地钉螺血淋巴细胞构成和透明细胞形态差异无统计学意义(P0.05),君山钉螺颗粒细胞长径和核质比均小于普格钉螺。
[Abstract]:The blood lymphocytes of Oncomelania hupensis (Oncomelania hupensis) collected from Junshan District, Yueyang City, Hunan Province and Puge County, Xichang City, Sichuan Province were collected and observed after Ji's staining, and their cell types were identified and counted. The morphological characteristics of different types of blood lymphocytes were measured. The results showed that the blood lymphocytes of Oncomelania hupensis could be divided into five types: eosinophilic clear cells, eosinophilic small transparent cells, basophilic small granulosa cells and basophilic large granular cells according to the cell acidity and alkalinity and intracellular granular properties of Oncomelania hupensis. Among the 5 types of cells, the percentage of eosinophilic small transparent cells was the largest, which was 36.7% (229 / 624) in Oncomelania hupensis and 31.7% (257 / 810) in Oncomelania Puge. The second was basophilic transparent cells, which were 23.1% (144 / 624) in Oncomelania hupensis and 24.4% (198 / 810) in Oncomelania Puge. The percentage of basophilic large granulosa cells was the lowest, which was 9.3% (58 / 624) and 11.6% (94 / 810), respectively. In morphology, the length diameter of eosinophilic large transparent cells was the largest, the nuclear / cytoplasmic ratio was the smallest, the eosinophilic small transparent cells were the second, the basic cells were smaller in length diameter and larger in nucleoplasm. There was no significant difference in blood lymphocyte composition and clear cell morphology between the two places (P0.05). The diameter and nucleoplasmic ratio of granulosa cells of Oncomelania hupensis were smaller than those of Oncomelania pumila.
【作者单位】：复旦大学公共卫生学院流行病学教研室公共卫生安全教育部重点实验室;复旦大学热带病研究中心;
【基金】：国家自然科学基金(No.30872159) 国家科技重大专项(No.2012ZX10004-220,2008ZX10004-011)~~
【分类号】：R184

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