浙江省甲3亚型流感流行与猪型流感的关系探讨

发布时间：2019-05-18 02:36

【摘要】： 背景和目的: 流行性感冒(influenza)是由流感病毒(Influenza Virus)引起的,具有高度传染性的急性呼吸道传染病。由于流感病毒基因的高度变异性,使该病在人群中发病率高且易发生流行。2009年春季开始的甲型H1N1流感流行再次使流感病毒成为全球关注的热点。甲型流感病毒,是危害人类健康的重要病原体之一,同时中国地区,又是世界上流感变异株的发源地之一,所以对其变异规律进行深入细致地分析研究,在流感的防治工作中具有极为重要的意义。猪流感(Swine influenza,SI)是由猪流感病毒(Swine influenza virus,SIV)引起的急性猪呼吸道传染病。猪具有同时感染禽和人流感病毒的能力,被认为是新亚型流感病毒产生的中间宿主和“基因混合器”。而且,猪流感病毒具有感染人和禽的能力,因此,猪流感具有重要的兽医和人类公共卫生学意义。本研究基于这一背景,就浙江省近十年来流感流行的甲型H3N2代表株,与猪型H3N2株在主要抗原基因间进行比较分析,探讨浙江省甲3亚型流感病毒与猪流感病毒之间的关系。材料和方法: 对浙江省近十年来流感流行期间分离的甲3亚型流感代表性毒株,采用逆转录-聚合酶链反应(Reverse transcription-Polymerase chain reaction ,RT-PCR)扩增病毒血凝素(Haemagglutinin, HA)和神经氨酸酶(Neuraminidase, NA)基因,进行核苷酸序列测定,并与其它同期的人源及猪源H3N2流感毒株的相应序列用BioEdit(version 5.0.9.1)和MEGA4.0软件作同源性与进化树分析。(其它同期的人源及猪源H3N2流感毒株的HA1、NA序列,由美国NCBI的GenBank下载。) 结果: 浙江省近十年来甲3亚型流感流行中的代表株A3/浙江(Zhejiang,ZJ)/10/98、A3/Zhejiang/6/99、A3/Zhejiang/8/02与猪流感A3/SW/安大略( Ontario,ON)/130/97、A3/SW/香港(Hongkong,HK)/4361/99、A3/SW/Hongkong/74/02在HA1区的同源性分别为99.1%、99.4%、99.4%;在NA区,A3/Zhejiang/10/98、A3/Zhejiang/6/99、A3/Zhejiang/8/02与猪流感A3/SW/Ontario/130/97、A3/SW/Henan/S4/01、A3/SW/HongKong/411//02的同源性分别达到98.2%、99.3%、99.3%。二者之间均呈现很高的同源性,有的远远高于它与同期人流感毒株的同源性,在基因系统进化树上也呈现这一状况。结论: 浙江省近十年来甲3亚型流感流行的毒株与猪流感的某些毒株之间存在着密切的联系,猪流感与人流感之间的关系值得深入研究。
[Abstract]:Background and objective: influenza (influenza) is an acute respiratory infectious disease caused by influenza virus (Influenza Virus). Because of the high variability of influenza virus gene, the incidence of influenza virus is high and easy to occur in the population. The influenza A H1N1 epidemic, which began in the spring of 2009, has once again made influenza virus become the focus of global attention. Influenza A virus is one of the important pathogens endangering human health. At the same time, China is also one of the birthplaces of influenza mutants in the world, so the variation law of influenza A virus is analyzed and studied in depth and detail. It is of great significance in the prevention and treatment of influenza. Swine flu (Swine influenza,SI) is an acute respiratory infectious disease caused by swine flu virus (Swine influenza virus,SIV. Pigs have the ability to infect both avian and human influenza viruses, and are considered to be intermediate hosts and "gene mixers" for the production of new subtypes of influenza viruses. Moreover, swine flu virus has the ability to infect people and birds, so swine flu has important veterinary and human public health significance. Based on this background, the representative strains of influenza A H3N2 in Zhejiang Province in recent ten years were compared with swine H3N2 strains in the main antigen genes, and the relationship between influenza A 3 subtypes influenza virus and swine influenza virus in Zhejiang Province was discussed. Materials and methods: viral hemagglutinin (Haemagglutinin,) was amplified by reverse transcription-polymerase chain reaction (Reverse transcription-Polymerase chain reaction, RT-PCR) against representative influenza A 3 strains isolated during influenza epidemic in Zhejiang Province in recent ten years. HA) and neurosinase (Neuraminidase, NA) gene were sequenced, and the homology and evolution tree were analyzed by BioEdit (version 5.0.9.1) and MEGA4.0 software with the corresponding sequences of other human and swine H3N2 influenza strains in the same period. (HA1,NA sequences of other human and swine H3N2 influenza strains were downloaded by GenBank of NCBI in the United States.) Results: the representative strains A3 / Zhejiang (Zhejiang,ZJ) / 10: 98, A3 / Zhejiang (6 / 99), A3 / Zhejiang (8 / 02) and swine flu A3/SW/ Ontario (Ontario,) in Zhejiang Province in the past ten years were the representative strains of influenza A _ 3 / Zhejiang (Zhejiang) / 10 / 98, A3 / Zhejiang / 99, A3 / Zhejiang / 8 / 02 and swine flu A3/SW/ Ontario (Ontario) in the past ten years. The homology of ON / 130 / 97, A3 / SW/ Hong Kong (Hongkong,HK) / 4361 / 99, A3 / SWHongkong74 / 02 in HA1 region was 99.1%, 99.4% and 99.4%, respectively. In Na district, the homology of A3 Zhejiang 98, A3 Zhejiang 699, A3 Zhejiang 802 and swine flu A3 SWOntuo 13097, A3 SWHenan S4 01, A3 SWHongKong411 / 02 is 98.2%, respectively, and the homology of A3 Zhejiang 802 with swine flu A3 SWHenan S401, A3 SWHongKong41102 is 98.2%, 98.2%, 98.2%, 98.2%, 98.2%, 98.2%, 98.2%, 98.2%, 98.2%, 98.2%, 98.2%, 98.2%, 98.2%, 98.2%, 98.2% and 98.2%, respectively. 99.3%, 99.3%. Both of them showed high homology, some of which were much higher than their homology with human influenza strains in the same period, and also showed this situation in the genetic phylogenetic tree. Conclusion: there is a close relationship between the epidemic strains of influenza A 3 and some strains of swine flu in Zhejiang Province in recent ten years, and the relationship between swine flu and human influenza is worthy of further study.
【学位授予单位】：宁波大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R181.3;S852.65

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