乙肝疫苗接种后不同应答人群的T细胞亚群观察

发布时间：2019-05-20 03:36

【摘要】： 目的通过对乙肝疫苗接种后不同免疫应答人群T细胞亚群的检测,探讨乙肝疫苗接种后免疫应答与T细胞亚群活化的关系。方法采集18例乙肝疫苗接种应答者(应答者)、22例乙肝疫苗接种无应答者(无应答者)、10例未接受乙肝疫苗接种的健康对照者(对照者)全血,用流式细胞术对其外周血单核细胞的表面标志物:CD69、CD4、CD8、CD25等进行检测。计算不同T细胞亚群百分比,如CD69+、CD4+、CD8+T细胞百分比及CD4+CD25+Treg细胞数。结果 1、应答者,无应答者,对照者CD4+CD69+T细胞占CD4+T细胞的百分比分别为1.81±1.33%、0.64±0.48%、和0.56±0.39%,应答者CD4+T细胞中CD69的表达明显高于对照者(P＜0.01),也明显高于无应答者。但无应答者与对照者间差异无显著性意义(p＞0.05)。各组CD8+CD69+T细胞占CD8+T细胞的百分比分别为0.43±0.25%、3.05±1.69%、和3.11±2.41%,应答者与对照者和无应答者比较,差异均有非常显著性意义(p＜0.01)。 2、应答者,无应答者,对照者CD3+CD4+T细胞占CD3+T细胞的百分比分别为50.84±6.36%、53.67±6.68%、和51.02±9.30%,各组结果比较,差异没有显著性意义(p＞0.05);各组CD3+CD8+T细胞占CD3+T细胞的百分比分别为41.02±4.41%、42.69±7.35%、和42.36±8.34%,统计学分析,组间结果的差异无显著性意义(p＞0.05)。 3、应答者,无应答者,对照者CD4+CD25+Treg细胞占CD4+T细胞的百分比分别为0.59±0.46%、0.77±0.40%、和1.30±1.44%,统计学分析,组间结果的差异无显著性意义(p＞0.05)。结论 1.乙肝疫苗接种后无应答与Th细胞早期活化不足有关。 2.乙肝疫苗接种后无应答可能与抑制性T细胞活化增加有关。 3.CD4+CD25+Treg细胞也可能参与了乙肝疫苗接种应答的负性调节,但由于研究结果差异无统计学显著性意义,其确切的意义还需进一步扩大样本数量进行研究。
[Abstract]:Objective to investigate the relationship between immune response and activation of T cell subsets in different immune responders after hepatitis B vaccine vaccination. Methods whole blood samples were collected from 18 hepatitis B vaccine responders (responders), 22 hepatitis B vaccinated non-responders (non-responders) and 10 healthy controls (controls) who were not vaccinated with hepatitis B vaccine, and the whole blood samples were collected from 18 hepatitis B vaccine responders (respondents), 22 hepatitis B vaccine non-responders (non-responders) and 10 healthy controls (controls). The surface markers of peripheral blood monocytes, such as CD69,CD4,CD8,CD25, were detected by flow cytometry. The percentages of different T cell subsets, such as CD69, CD4, CD8 T cells and the number of CD4 CD25 Treg cells, were calculated. Results 1. The percentage of CD4 CD69 T cells in CD4 T cells was 1.81 卤1.33%, 0.64 卤0.48% and 0.56 卤0.39%, respectively. The expression of CD69 in CD4 T cells of responders was significantly higher than that of controls (P < 0.01), and it was also significantly higher than that of non-responders. However, there was no significant difference between the non-responders and the controls (p > 0.05). The percentage of CD8 CD69 T cells in CD8 T cells in each group was 0.43 卤0.25%, 3.05 卤1.69% and 3.11 卤2.41%, respectively. there was significant difference between the responders and the controls and the non-responders (p < 0.01). 2, the percentage of CD3 CD4 T cells in CD3 T cells was 50.84 卤6.36%, 53.67 卤6.68%, and 51.02 卤9.30%, respectively. there was no significant difference between the two groups (p > 0.05), and the percentage of CD3 T cells in control group was 50.84 卤6.36%, 53.67 卤6.68% and 51.02 卤9.30%, respectively. there was no significant difference between the two groups (p > 0.05). The percentage of CD3 CD8 T cells in CD3 T cells in each group was 41.02 卤4.41%, 42.69 卤7.35% and 42.36 卤8.34%, respectively. there was no significant difference between the two groups. 3, the percentage of CD4 CD25 Treg cells in CD4 T cells was 0.59 卤0.46%, 0.77 卤0.40% and 1.30 卤1.44%, respectively. There was no significant difference in the results between the two groups (p > 0.05). Conclusion 1. The lack of response after hepatitis B vaccination was related to the lack of early activation of Th cells. 2. No response after hepatitis B vaccination may be related to the increase of inhibitory T cell activation. 3.CD4 CD25 Treg cells may also be involved in the negative regulation of hepatitis B vaccination response, but because there is no significant difference in the results of the study, its exact significance needs to be further expanded to study the number of samples.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R186

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