淫羊藿苷延缓衰老过程中对ATM-Chk2-p53通路的调节作用

发布时间：2018-01-02 17:45

本文关键词：淫羊藿苷延缓衰老过程中对ATM-Chk2-p53通路的调节作用　出处：《老年医学与保健》2016年06期 　论文类型：期刊论文

【摘要】：目的观察淫羊藿苷对老年小鼠DNA损伤检验点ATM-Chk2-p53通路的调节作用。方法 21月龄C57BL/6老年小鼠随机分为老年对照组和淫羊藿苷组,同时取3月龄小鼠作为青年对照组;淫羊藿苷组小鼠给予含0.02%淫羊藿苷的饲料喂养,老年对照组和青年对照组小鼠给予普通正常小鼠饲料喂养。利用淫羊藿苷干预3个月后,在实验之前部分小鼠先进行5Gy X线照射6 h,然后所有小鼠麻醉后处死,收集标本进行相关实验。取小鼠脾脏,抽提小鼠脾组织中的总RNA,利用Real-time PCR方法检测DNA损伤检验点上关键分子ATM、Chk2和p53的mRNA表达水平;提取小鼠脾组织中的总蛋白,采用Western blot方法检测DNA损伤检验点上p-ATM、p-Chk2和p-p53蛋白的表达。结果在电离辐射情况下,老年小鼠ATM、Chk2和p53基因的mRNA表达的增高倍数明显较青年小鼠低,同时老年小鼠p-ATM、p-Chk2和p53蛋白的表达水平较青年小鼠降低;而淫羊藿苷能够提高老年小鼠在电离辐射情况下ATM、Chk2、p53基因的mRNA表达水平,并且能够提高老年小鼠p-ATM、p-Chk2和p-p53蛋白表达水平。结论淫羊藿苷能够激活DNA损伤检验点ATM-Chk2-p53通路功能,这可能是淫羊藿苷延缓小鼠衰老的重要机制之一。
[Abstract]:Objective to observe the regulatory effect of icariin on ATM-Chk2-p53 pathway at DNA damage test point in aged mice. 21-month-old C57BL / 6 aged mice were randomly divided into two groups: aged control group and icariin group. At the same time, the 3-month-old mice were used as the control group. The mice of icariin group were fed with 0.02% icariin diet, the aged control group and the young control group were fed with normal diet. The mice were treated with icariin for 3 months. Before the experiment, some mice were exposed to 5 Gy X-ray for 6 h, then all mice were anesthetized and killed. The spleen was collected and the total RNA was extracted from the spleen of the mice. Real-time PCR method was used to detect the mRNA expression level of the key molecules ATMN Chk2 and p53 on the DNA damage detection site. Total protein was extracted from mouse spleen tissue and p-ATM was detected by Western blot method. The expression of p-Chk2 and p-p53 proteins in aged mice was significantly lower than that in young mice under ionizing radiation. At the same time, the expression of p-ATMN p-Chk2 and p53 protein in aged mice was lower than that in young mice. Icariin could increase the expression of mRNA of ATMN Chk2P p53 gene in aged mice under ionizing radiation, and it could also increase p-ATM level in aged mice. Conclusion Icariin can activate the function of ATM-Chk2-p53 pathway at DNA damage test point. This may be one of the important mechanisms of icariin delaying aging in mice.
【作者单位】：杭州师范大学附属医院干部科;上海市第八人民医院中医科;
【分类号】：R339.3~+8
【正文快照】： 目前衰老和抗衰老研究是当今生物医学研究领域的热点。限制饮食被证实能够延长低等动物和哺乳动物的寿命,但在临床上实施还面临着多种困难,且有可能引起健康问题。笔者前期研究M从细胞水平、低等动物、哺乳动物等层次发现补肾中药淫羊藿的有效成分淫羊藿总黄酮和淫羊藿苷具

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