七氟醚对新生大鼠下丘脑-垂体-肾上腺皮质轴发育的影响

发布时间：2018-01-13 18:42

本文关键词：七氟醚对新生大鼠下丘脑-垂体-肾上腺皮质轴发育的影响　出处：《临床麻醉学杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的研究新生期七氟醚暴露对下丘脑-垂体-肾上腺皮质(hypothalamic-pituitary-adrenal,HPA)轴发育及功能的影响。方法新生雄性SD大鼠54只,6日龄,随机分为三组:对照组(C组)、七氟醚组(S组)和布美他尼+七氟醚组(BS组),每组18只。C组:吸入100%氧气6h;S组:吸入2.1%七氟醚6h;BS组:吸入2.1%七氟醚6h,在麻醉前30min和麻醉后3h注射布美他尼。6h后,每组随机选取6只处死收集血清,采用ELISA法测定皮质酮浓度;各组剩余大鼠于第60天行高架十字迷宫测试,第65天行束缚应激30 min,并在应激前后取尾静脉血,采用ELISA法测定血清皮质酮浓度。结果麻醉后S组血清皮质酮浓度明显高于C组(P0.01);BS组血清皮质酮浓度明显低于S组(P0.01)。S组进入开放臂次数明显少于C组(P0.05),开放臂停留时间明显短于C组(P0.05),应激前后血清皮质酮浓度明显高于C组(P0.05或P0.01);BS组进入开放臂次数明显多于S组,开放臂停留时间明显长于S组(P0.05),应激前后血清皮质酮浓度明显低于S组(P0.05)。结论新生期七氟醚麻醉可导致HPA轴功能亢进,其机制与GABAA受体相关。
[Abstract]:Objective to study the effect of sevoflurane exposure on hypothalamic-pituitary-adrenal (Hypothalamic-pituitary-adrenal). Methods 54 male SD rats were randomly divided into three groups: control group (C group). Sevoflurane group (n = 18) and bumetanil sevoflurane group (n = 18) and bumetanil sevoflurane group (n = 18). Group C: inhaled oxygen for 100% hours; Group S: inhaled 2.1% sevoflurane for 6 hours; BS group: after inhaling 2.1% sevoflurane for 6 hours, bumetanil was injected 30 minutes before anesthesia and 3 hours after anesthesia. 6 rats in each group were randomly killed to collect serum. The concentration of corticosterone was determined by ELISA. The remaining rats in each group were tested by elevated cross maze on the 60th day and 30 mins by restraint stress on the 65th day, and the blood of the tail vein was taken before and after the stress. Results the concentration of corticosterone in group S was significantly higher than that in group C (P 0.01) after anesthesia. The serum corticosterone concentration in BS group was significantly lower than that in group S (P 0.01). The number of open arm entry in group S was significantly lower than that in group C (P 0.05), and the retention time of open arm was significantly shorter than that in group C (P 0.05). The concentration of corticosterone in serum before and after stress was significantly higher than that in group C (P0.05 or P0.01). The number of open arm entry in BS group was significantly higher than that in S group, and the open arm stay time was significantly longer than that in S group (P0.05). Conclusion sevoflurane anaesthesia in newborn stage can lead to hyperfunction of HPA axis, and its mechanism is related to GABAA receptor.
【作者单位】：南华大学医学院寄生虫学教研室;南华大学医学院组织学与胚胎学教研室;
【基金】：国家自然科学基金(81301144) 国家留学基金(201608430251)
【分类号】：R726.1
【正文快照】： 全球每年有数百万婴幼儿需要在麻醉下接受各种检查或外科手术操作[1]。孕后期至婴幼儿时期人类大脑处于高速发育时期,具有较强的可塑性[2],麻醉药物对大脑内的未成熟神经元发育造成远期影响已经得到广泛关注。临床研究表明,新生期七氟醚暴露可导致远期认知功能受损和情绪障碍[

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