不同局部肿胀麻醉液对体外培养人脂肪干细胞增殖与成脂分化的影响

发布时间：2018-01-14 22:25

本文关键词：不同局部肿胀麻醉液对体外培养人脂肪干细胞增殖与成脂分化的影响　出处：《中国组织工程研究》2015年50期 　论文类型：期刊论文

【摘要】：背景:获取脂肪术中需要应用不同的局部肿胀麻醉液,其可能会对细胞生物特性产生一定的影响。目的:探讨体外培养条件下不同局部肿胀麻醉液对人脂肪干细胞增殖与成脂分化的影响。方法:取第3代人脂肪干细胞分别用布比卡因、罗哌卡因、利多卡因局部肿胀麻醉液进行培养,对照组用低糖DMEM培养基进行培养,检测各组干预不同时间人脂肪干细胞增殖活性、细胞上清乳酸脱氢酶活性和成脂分化比例。结果与结论:不同局部肿胀麻醉液干预后1,2,3,4,5 d细胞上清乳酸脱氢酶活性值显著高于对照组(P0.05),布比卡因组显著高于罗哌卡因组、利多卡因组(P0.05)。各局部肿胀麻醉液组细胞生长吸光度值均低于对照组(P0.05),且布比卡因组显著低于罗哌卡因组、利多卡因组(P0.05)。各组人脂肪干细胞成脂分化比例差异无显著性意义(P0.05)。结果表明,局部肿胀麻醉液(布比卡因、罗哌卡因、利多卡因)均能够抑制人脂肪干细胞增殖,但不会对细胞成脂分化产生明显的影响。
[Abstract]:Background: different local swelling anesthetics are needed to obtain fat. Objective: to investigate the effects of different local swelling anesthetic fluids on the proliferation and adipogenic differentiation of human adipose stem cells in vitro. The third generation of human adipose stem cells were treated with bupivacaine. Ropivacaine and lidocaine were cultured in local swelling anaesthesia, while those in control group were cultured in low glucose DMEM medium. The proliferation activity of human adipose stem cells was detected in each group. Results and conclusion: after intervention of different local swelling anesthetic solution, lactic dehydrogenase activity and adipogenic differentiation ratio of the supernatant were 1 ~ 2o _ 3 ~ (4). The activity of lactate dehydrogenase in the supernatant of 5 days was significantly higher than that in the control group (P 0.05), and that in the bupivacaine group was significantly higher than that in ropivacaine group. The cell growth absorbance of lidocaine group was lower than that of control group, and that of bupivacaine group was significantly lower than ropivacaine group. There was no significant difference in adipogenic differentiation of human adipose stem cells in lidocaine group (P 0.05). The results showed that bupivacaine and ropivacaine were locally swollen anesthetic fluid. Lidocaine can inhibit the proliferation of human adipose stem cells, but has no obvious effect on adipogenic differentiation.
【作者单位】：滨州市人民医院麻醉科;滨州市人民医院特检科;滨州医学院附属医院麻醉科;
【分类号】：R614
【正文快照】： 0引言Introduction脂肪干细胞具有免疫原性低的特点,可以在一定条件下发生分化,形成软骨细胞、成骨细胞以及脂肪细胞等[1],被广泛应用于骨缺损修复和创面治疗等。黄成龙等[2]指出骨质疏松症患者的骨缺损修复难度较大,常规治疗大多无法获得理想的效果,而在一定条件下诱导脂肪干

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