短尾蝮蛇异氟烷气体麻醉方法介绍

发布时间：2018-01-15 00:18

本文关键词：短尾蝮蛇异氟烷气体麻醉方法介绍　出处：《四川动物》2015年05期 　论文类型：期刊论文

【摘要】：蛇类因其独有的形态、行为及生理特征而受到研究者的关注。麻醉作为一种技术手段,是对蛇类进行很多其他实验的前提。然而,目前对蛇类等非模式动物的麻醉方法研究远不及标准实验动物(如小鼠)成熟。本文以短尾蝮蛇Gloydius brevicaudus气体麻醉为例,介绍一种适用于蛇类的安全麻醉方法,以有利于相关动物学研究的顺利开展。以预麻醉、诱导麻醉和苏醒3个步骤分别记录到的时间作为数据参数分析,20号短尾蝮蛇样本达到完全麻醉状态平均需要时间33.05 min,对异氟烷耐受性强的个体在1 h内也能麻醉完全,平均苏醒需要的时间为41.80min。麻醉用的时间越长,苏醒需要的时间也越长,进行相关实验操作需要综合考虑麻醉和苏醒的时间。
[Abstract]:Snakes are concerned by researchers because of their unique morphology, behavior and physiological characteristics. Anesthesia, as a technical means, is the premise of many other experiments on snakes. At present, the anesthetic methods of non-model animals such as snakes are far less mature than those of standard experimental animals (such as mice). In this paper, Gloydius brevicaudus gas anesthesia of Agkistrodon halys is taken as an example. A safe anesthetic method suitable for snakes was introduced in order to facilitate the smooth development of related zoological research. The time recorded in the three steps of preanesthesia induction anesthesia and recovery was used as data parameter analysis. It took an average time of 33.05 mins to reach the state of complete anaesthesia in 20 samples of Agkistrodon halys. Individuals with strong tolerance to isoflurane could also be anesthetized completely within 1 h. The average recovery time is 41.80 mins. The longer the anaesthesia time, the longer the recovery time. The time of anaesthesia and awaking need to be considered synthetically in the experiment operation.
【作者单位】：中国科学院成都生物研究所;中国科学院大学;
【基金】：国家自然科学基金项目(31301882) 中国博士后科学基金项目(2013M542301) 中国科学院2013年度“西部之光”人才培养计划
【分类号】：Q95-33
【正文快照】： 蛇类作为爬行纲动物的重要组成部分,一直以来便是广大学者关注的对象,从系统学到生态学,从行为学到生理学,已有的研究涉及到了动物学研究的大多数领域(Ding et al.,2011;Geng et al.,2011;Chen et al.,2012)。动物学研究中的某些环节,如外科手术,常常需要对研究对象进行麻醉。

【参考文献】