活血散干预大鼠骨折模型早期骨愈合的实验研究

发布时间：2018-01-16 19:11

本文关键词：活血散干预大鼠骨折模型早期骨愈合的实验研究　出处：《南京中医药大学》2017年硕士论文　论文类型：学位论文

【摘要】：[目的]通过大鼠急性新鲜股骨骨折损伤模型的建立,运用南京市中医院院内制剂活血散软膏作为外敷治疗骨折药物,通过不同时间段测定用药前后大鼠血液流变学、血浆粘度和血清碱性磷酸酶(ALP)、钙(Ca)、磷(P)的变化,同时观察组织病理学和X线扫描成像的改变,探讨活血散促进骨折愈合的机理,为临床中药外治方法提供了基础理论依据。[方法]根据中药药理实验学实验方法,造模前一天,大鼠左侧臀部及大腿后侧用8%硫化钠脱毛剂脱毛。用20%的乌拉坦溶液按体重(5ml/kg)行腹腔注射将大鼠浅麻醉,大鼠反抗能力减弱即为麻醉显效;大鼠呈仰卧位,固定左侧大腿呈外展内旋的姿势,将造模支架固定于此并调节铁碇,将在大腿中部用钝骨刀加压,助手提起500g的砝码至30cm处,让砝码沿导引杆以自由落体的方式与钢板撞击,力传导至钝骨刀片上,撞击股骨中段造成骨折。造模后立即拍摄患肢正位X线片,了解骨折类型及移位程度;要求骨折类型为横断或短斜型,显示造模成功。打击粉碎性骨折及皮肤破损的大鼠予以剔除。选取造模成功大鼠36只随机分为两大组:A组对照组18只,外涂凡士林/羊毛脂2:1混合膏5g加特殊夹板固定,根据取材时间再随机分成a,b,c3组(给药后第10,20,30d);B组实验组18只,与骨折处外涂活血散软膏5g加特殊夹板固定,根据取材时间再随机分成a,b,c3组(给药后第10,20,30d)。造模成功1小时后实施相关治疗处理。在用药后的第10天、20天、30天,各组任取大鼠6只,麻醉后采集股动脉血进行血液流变学、血浆粘度及血生化测定,取血结束后采用牵拉使颈椎脱臼的方法快速处死大鼠,然后解剖分离骨折段股骨,取股骨全长标本进行X线扫描成像、骨痂病理组织形态学检查。[结果]活血散膏组在对骨折模型大鼠血浆粘度、血浆粘度、血清ALP、Ca、P含量的影响,以及骨折模型大鼠骨痂组织形态学、骨折模型大鼠股骨X线片的影响均优于模型组。活血散在治疗20、30天时,可以显著改善骨折模型大鼠的血液流变学,降低全血ls-l、5s-l、30s-1的切变率,可以显著降低骨折模型大鼠的血浆粘度,可以显著升高骨折模型大鼠血清ALP、Ca、P含量。在不同时间节点上活血散组的骨折断端病理切片均可见软骨性骨痂、小梁状骨的产生明显优于对照组,X线片上显示的骨痂生长影也明显优于对照组。[结论]活血散局部外用对大鼠骨折模型早期骨愈合有明显促进作用,其作用机理可能与改善血循环和促进成骨代谢相关。
[Abstract]:[Objective] to establish a model of acute fresh femoral fracture injury in rats and to use Huoxue Powder ointment as an external application drug in Nanjing Hospital of traditional Chinese Medicine. The changes of hemorheology, plasma viscosity and serum alkaline phosphatase (ALPX), calcium and calcium (Cam) in rats before and after treatment were measured at different time periods. At the same time, histopathology and X-ray scanning imaging were observed, and the mechanism of promoting fracture healing by Huoxue Powder was discussed, which provided the basic theoretical basis for the external treatment of traditional Chinese medicine. [Methods] according to the experimental method of pharmacology of traditional Chinese medicine, the model was made one day before. The rats left buttocks and thighs were treated with 8% sodium sulphide hair remover. The rats were anesthetized by intraperitoneal injection with 20%% uratan solution (5 ml / kg). The decrease of resistance ability of rats is the obvious effect of anesthesia. The rats presented supine position, fixed the left thigh in the position of abduction and internal rotation, fixed the scaffold and adjusted the iron Anchorage, and pressed it with a blunt bone knife in the middle of the thigh, and the assistant lifted 500g weight to 30cm. Let the weight strike the steel plate along the guide rod in the way of free falling body, and the force is transmitted to the blunt bone blade, and the fracture is caused by hitting the middle part of the femur. Immediately after making the model, the orthostatic X-ray film of the affected limb is taken to understand the type of fracture and the degree of displacement. The fracture type was required to be transverse or short oblique type, which showed that the model was successful. The rats that hit the comminuted fracture and the skin damaged were eliminated. Thirty-six successful rats were randomly divided into two groups: group A, group A, control group, n = 18. The Vaseline / lanolin 2: 1 mixed cream was fixed with special splint at 5g. According to the time of extraction, it was randomly divided into 3 groups (10 ~ 20 ~ 30 days after administration). In group B, 18 rats were treated with Huoxue Powder ointment at the fracture site and fixed with special splint. According to the time of extraction, they were randomly divided into 3 groups (1020). 30 days after the successful establishment of the model 1 hour after the implementation of the relevant treatment. 10 days after the use of drugs 20 days to 30 days, each group of 6 rats, the femoral artery blood was collected after anesthesia for hemorheology. The plasma viscosity and blood biochemistry were measured. The rats were killed quickly by pulling the cervical vertebrae dislocated after the blood was taken. Then the femur was dissected and the whole femur specimens were taken for X-ray imaging. Histopathological examination of callus. [Results: the effect of Huoxue Powder ointment on plasma viscosity, plasma viscosity and the content of serum ALP in rats with fracture, and the histomorphology of callus in fracture model rats. The effect of X-ray film of femur in fracture model rats was better than that in model group. The hemorheology of fracture model rats could be significantly improved and the whole blood ls-ln 5s-l could be reduced by Huoxue Powder after 20 ~ 30 days of treatment. The shear rate of 30 s ~ (-1) could significantly decrease the plasma viscosity and increase the serum ALP ~ (+) Ca in the fracture model rats. P content. Cartilage callus could be seen in pathological sections of fracture broken end in Huoxue Powder group at different time points. Trabecular bone formation was obviously superior to the growth shadow of bone callus on X ray film of control group. [Conclusion: the local application of Huoxue Powder can obviously promote bone healing in the early stage of fracture model in rats, and its mechanism may be related to improving blood circulation and promoting osteogenesis metabolism.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R285.5;R-332

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