银杏达莫对缺血-再灌注损伤心肌保护作用的钙稳态调控机制

发布时间：2018-01-19 07:23

本文关键词： 银杏达莫尼卡地平氯化镧缺血-再灌注钙超载线粒体　出处：《中药材》2015年12期 　论文类型：期刊论文

【摘要】：目的:探讨银杏达莫对大鼠离体心脏缺血-再灌注损伤心肌保护作用的钙稳态调控机制。方法:雄性SD大鼠40只随机分成5组:正常对照组、缺血-再灌注模型组、银杏达莫组、尼卡地平+银杏达莫组及氯化镧+银杏达莫组。实验动物麻醉后取心脏,经Langendorff装置灌流,观察各组5个时间点(预灌30 min稳定点,缺血30 min,再灌5、30、60 min)的心功能指标(HR、LVSP、±dp/dtmax),同时收集各时间点冠脉流出液,检测其中LDH、CK活性及Ca2+浓度。实验结束取部分心肌制备线粒体,检测其中Ca2+、IDH及α-OGDH含量。组织切片观察心肌组织病理学变化。结果:与模型组比较,银杏达莫组在心脏缺血-再灌注各时间点各项心功能指标(LVSP×HR、±dp/dtmax)均有所改善;心肌LDH、CK的释放及线粒体内Ca2+、IDH、α-OGDH含量均有所降低。以上保护作用可被尼卡地平和氯化镧抑制。组织切片结果显示模型组心肌组织有明显的损伤,银杏达莫、尼卡地平+银杏达莫及氯化镧+银杏达莫对心肌损伤均有修复作用,其中以银杏达莫的修复作用最明显。结论:银杏达莫对缺血-再灌注心肌有保护作用,其机制可能与维持细胞钙稳态及线粒体能量代谢功能有关。
[Abstract]:Objective: to investigate the calcium homeostasis regulation mechanism of Ginkgo biloba on myocardial ischemia reperfusion injury in isolated rat heart. Methods: forty male SD rats were randomly divided into 5 groups: normal control group. Ischemia-reperfusion model group, ginkgo biloba group, nicardipine ginkgo biloba dimer group and lanthanum chloride Ginkgo biloba dimer group. The hearts of the experimental animals were taken after anesthesia and perfused with Langendorff device. The cardiac function parameters of each group were observed at 5 time points (30 min preperfusion, 30 min ischemia and 60 min reperfusion). At the same time, the coronary effluents were collected at different time points to detect the activity of LDH-CK and the concentration of Ca2. At the end of the experiment, some of the myocardium were taken to prepare mitochondria and the Ca2 was detected. IDH and 伪 -OGDH contents. Histopathological changes of myocardium were observed by histopathological sections. Results: compared with the model group. In Ginkgo biloba group, LVSP 脳 HR, 卤DP / dtmaxwere improved at each time point after ischemia reperfusion. The release of CK in myocardium and Ca2 / IDH in mitochondria. The content of 伪 -OGDH decreased. The above protective effects were inhibited by nikadipine and lanthanum chloride. The results of tissue sections showed that the myocardial tissue of the model group had obvious damage, Ginkgo biloba dimer. Both nicardipine and lanthanum ginkgo chloride can repair myocardial injury. Conclusion: ginkgo biloba has protective effect on ischemia-reperfusion myocardium. The mechanism may be related to the maintenance of calcium homeostasis and mitochondrial energy metabolism.
【作者单位】：皖南医学院生理教研室;
【基金】：国家自然科学基金(81172790) 安徽省自然科学研究重点基金(KJ2013A251) 安徽省质量工程项目(2013jyxm127) 皖南医学院重点科研项目培育基金(WK2012Z01);皖南医学院中青年基金(WK201507)
【分类号】：R285.5
【正文快照】： 心肌缺血-再灌注损伤(myocardial ischemiareperfusion injury,MIRI)在体外循环心脏手术的复灌、心跳骤停者复苏及心肌梗死患者冠脉溶栓术等过程中均可发生,严重威胁患者的术后恢复和生命安全,关于MIRI的机制一般认为与氧自由基的产生、细胞内钙超载、线粒体能量代谢紊乱以及

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