氯胺酮对幼年小鼠空间学习记忆功能及海马脑区TSPO蛋白的影响

发布时间：2018-01-20 22:04

本文关键词： 氯胺酮学习记忆能力小胶质细胞 kDa转位蛋白　出处：《山东大学学报(医学版)》2015年04期 　论文类型：期刊论文

【摘要】：目的观察幼年小鼠连续多次接受氯胺酮麻醉后学习记忆认知功能及海马脑区内TSPO蛋白的表达情况。方法出生21 d的幼年小鼠24只随机分为对照组(n=12)和氯胺酮组(n=12),每天1次分别经腹腔注射生理盐水和氯胺酮,连续7 d。然后进行Morris水迷宫实验,包括4 d的适应性训练和1 d的空间探索实验,观察小鼠的学习记忆功能的改变。水迷宫行为学测试后处死小鼠,取双侧的海马脑区,采用Western blotting技术和免疫荧光双标技术检测小鼠海马内小胶质细胞的变化和TSPO表达水平。结果训练期3~4 d,氯胺酮组小鼠潜伏期明显长于对照组(P0.05)。探索实验显示,与对照组比较,氯胺酮组小鼠在平台所在象限滞留时间和经过次数明显减少(P0.05);Western blotting分析结果表明,氯胺酮组幼年小鼠海马小胶质细胞标记物Iba-1和TSPO蛋白表达明显增多;免疫荧光双重标记技术显示,氯胺酮组小鼠海马小胶质细胞发生了形态学的改变,TSPO蛋白的表达增强。结论氯胺酮组小鼠海马脑区内小胶质细胞被激活,与之共同表达的TSPO蛋白的表达增高,学习记忆等认知功能减退。
[Abstract]:Objective to observe the cognitive function of learning and memory and the expression of TSPO protein in hippocampus of young mice after repeated ketamine anesthesia. Methods Twenty-four young mice were randomly divided into control group (n = 24). N) and ketamine group (n = 12). Saline and ketamine were injected intraperitoneally once a day for 7 days, then Morris water maze test was performed, including adaptability training for 4 days and space exploration experiment for 1 day. The changes of learning and memory function in mice were observed. The mice were killed after water maze behavior test and bilateral hippocampal regions were taken. Western blotting technique and immunofluorescence double labeling technique were used to detect the changes of microglia and the expression of TSPO in the hippocampus of mice. The incubation period of ketamine group was longer than that of control group (P 0.05). The retention time and pass times of ketamine group in platform quadrant decreased significantly (P 0.05). The results of Western blotting analysis showed that the expression of Iba-1 and TSPO protein in hippocampal microglia of young mice in ketamine group was significantly increased. Immunofluorescence double labeling technique showed that there were morphological changes in hippocampal microglia in ketamine group and the expression of TSPO protein was enhanced. Conclusion Ketamine group can activate microglia in hippocampal area of mice. The expression of TSPO protein was increased and cognitive function such as learning and memory was decreased.
【作者单位】：潍坊医学院麻醉学系;解放军第八十九医院麻醉科;
【基金】：山东省教育厅课题(J13LL02)
【分类号】：R726.1
【正文快照】： 网络出版地址:http://www.cnki.net/kcms/detail/37.1390.R.20150309.1025.001.html术后认知功能障碍(postoperative cognitive dys-function,POCD)是麻醉手术后中枢神经系统常见的并发症之一,学习和记忆是认知功能的主要组成部分。研究显示,麻醉药可能是导致认知功能障碍的重

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