痛泻要方对溃疡性结肠炎大鼠下丘脑中IL-6,IL-6R表达的影响

发布时间：2018-02-04 09:30

  本文关键词： 痛泻要方 溃疡性结肠炎 白细胞介素-(IL-) 白细胞介素-受体(IL-R) 基因表达 蛋白表达　出处：《中国实验方剂学杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的:观察痛泻要方对溃疡性结肠炎(ulcerative colitis,UC)大鼠下丘脑中白细胞介素-6(IL-6)和白细胞介素-6受体(IL-6R)基因和蛋白表达的影响。方法:将SPF级Wistar大鼠运用复合方法进行溃疡性结肠炎造模后,随机分为5组:模型组,痛泻要方低、中、高剂量(2.75,5.5,11 g·kg~(-1))组,美沙拉嗪(5-ASA)组。药物处理大鼠21 d后麻醉处死,肉眼观察结肠大体形态损伤并进行评分。分离各组大鼠血清,酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测血清中IL-6含量,分离大鼠下丘脑组织并提取总RNA后,实时荧光定量PCR(Real-time polymerase chain reaction,Real-time PCR)法检测IL-6和IL-6R基因表达,免疫组织化学染色法检测下丘脑组织IL-6和IL-6R的分布及蛋白表达量。结果:ELISA结果显示,与空白组比较,模型组大鼠血清中IL-6含量明显升高,经过药物处理后,药物组血清中IL-6含量均有所下降,其中以痛泻要方高剂量组效果最为明显(P0.05)。Real-time PCR结果表明,模型组大鼠下丘脑组织中IL-6和IL-6R基因表达量明显高于空白组(P0.05),经药物治疗后,药物组IL-6和IL-6R基因表达均有所降低,其中以5-ASA组和痛泻要方高剂量组降低最为明显(P0.05)。免疫组织化学染色结果表明,与空白组比较,溃疡性结肠炎大鼠IL-6和IL-6R蛋白表达量明显增加(P0.05),药物处理可以降低大鼠下丘脑IL-6和IL-6R蛋白表达量,其中以5-ASA组和痛泻要方高剂量组降低最为明显(P0.05)。结论:痛泻要方对2,4,6三硝基苯磺酸(2,4,6-trinitrobenzene sulfonic acid,TNBS)/乙醇法UC大鼠下丘脑组织中IL-6和IL-6R基因和蛋白的表达具有明显抑制作用,提示痛泻要方治疗溃疡性结肠炎的机制可能与抑制下丘脑中IL-6/IL-6R信号通路的异常活化有关。
[Abstract]:Objective: to observe the effect of Tongxieyao recipe on ulcerative colitis of ulcerative colitis. Interleukin-6 (IL-6) and interleukin-6 receptor (IL-6R) in the hypothalamus of UC- rats. Methods: SPF grade Wistar rats were used to model ulcerative colitis. The rats were randomly divided into 5 groups: model group, low, medium and high dose of Tongxieyangfang (2.75 ~ 5.5g 路kg ~ (-1))) group. The rats were anesthetized and killed after 21 days of drug treatment. Gross colonic lesion was observed and evaluated with naked eye. Serum of rats in each group was separated. Enzyme linked immunosorbent assay (enzyme linked immunosorbent assay (Elisa)) was used to detect the content of IL-6 in serum. The rat hypothalamus tissue was isolated and total RNA was extracted. The real-time PCR(Real-time polymerase chain reaction was quantified by fluorescence. IL-6 and IL-6R gene expression were detected by Real-time PCR. The distribution and protein expression of IL-6 and IL-6R in hypothalamus were detected by immunohistochemical staining. The content of IL-6 in the serum of the model group was significantly higher than that of the control group. After drug treatment, the content of IL-6 in the serum of the drug group decreased. Among them, the effect of Tongxieyangfang group was the most obvious (P0.05N. Real-time PCR). The expression of IL-6 and IL-6R genes in hypothalamus of the model group was significantly higher than that of the blank group (P 0.05). The expression of IL-6 and IL-6R in the drug group was decreased, especially in the 5-ASA group and the high-dose group of Tongxieyangfang. The results of immunohistochemical staining showed that the expression of IL-6 and IL-6R in the drug group was significantly lower than that in the control group. Compared with the blank group, the expression of IL-6 and IL-6R protein increased significantly in ulcerative colitis rats (P0.05). Drug treatment can reduce the expression of IL-6 and IL-6R protein in hypothalamus of rats. Among them, 5-ASA group and Tongxieyao prescription high dose group were the most obvious decrease of P0.05.Conclusion: Tongxiieyao recipe has a significant effect on 2N 4N 6 trinitrobenzenesulfonic acid 2N 4. 6-trinitrobenzene sulfonic acid. The expression of IL-6 and IL-6R genes and proteins in the hypothalamus of UC rats was significantly inhibited by TNBS / ethanol. The results suggest that the mechanism of Tongxieyangfang in the treatment of ulcerative colitis may be related to the inhibition of abnormal activation of IL-6/IL-6R signal pathway in hypothalamus.
【作者单位】： 甘肃中医药大学;
【分类号】：R285.5
【正文快照】： [网络出版地址]http://www.cnki.net/kcms/detail/11.3495.R.20170215.0942.024.html[网络出版时间]2017-02-15 9:42溃疡性结肠炎(ulcerative colitis,UC)是发生于结肠和直肠处的一种非特异性炎症。由于目前较公认的致病因素有遗传、环境、免疫紊乱等,西医多运用激素、磺胺类，

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