小动物磁共振成像中不同麻醉方式的比较性研究

发布时间：2018-02-04 17:26

本文关键词： 磁共振成像麻醉动物实验　出处：《重庆医学》2015年34期 　论文类型：期刊论文

【摘要】：目的探讨在7.0T小动物磁共振研究中,注射麻醉与呼吸麻醉对实验动物和图像质量的影响及临床意义。方法选取80只200g左右的SD大鼠,随机分为两组,每组40只,其中,A组为注射麻醉组,B组为呼吸麻醉组。麻醉药物为异氟烷,预麻醉中麻醉剂量为0.5%,氧气为2lps,当动物深度麻醉后(双眼闭合,摇晃麻醉盒无反应时),倒计时2min,然后取出实验动物在检查床上进行固定,持续进行0.2%麻醉。固定所有动物时,均用医用纸胶带粘牢大鼠颈部,使大鼠头颅尽量与大鼠表面线圈接触,检查过程中所有动物均采用实时呼吸监控,进行常规头颅T1、T2、磁共振弥散加权成像(DWI)、灌注加权成像(PWI)成像。结果 A组实验动物在扫描过程中死亡4只,因呼吸逐渐上升,PWI检查前进行二次麻醉8只,B组实验动物均正常完成所有检查。B组实验动物较A组苏醒快;与A组比较,B组功能成像图像质量明显较好。结论实验中,小动物的状态可直接影响实验进程及实验结果的质量,而稳定、安全的麻醉是控制实验动物状态的有效方式。本研究中,呼吸麻醉可更好地控制大鼠的呼吸,并可保证动物的安全,从而提高实验的质量,为临床研究提供实验保障。
[Abstract]:Objective to investigate the effect of injection anesthesia and respiratory anesthesia on experimental animals and image quality in 7.0T small animals. Methods 80 SD rats of about 200g were selected. The rats were randomly divided into two groups, 40 rats in each group. Group A was injected anesthesia group, group B was respiratory anesthesia group, the anesthetic drug was isoflurane, the dose of preanesthesia was 0.5 and oxygen was 2lps. When the animals were under deep anesthesia (closed eyes, shook the anaesthesia box unresponsive, countdown 2 minutes, then took out the laboratory animals in the examination bed fixed, continued for 0.2% anesthesia. Fixed all the animals. Medical paper adhesive tape was used to stick the neck of the rat to make the rat head contact with the surface coil of the rat as far as possible. During the examination all animals were monitored by real-time breathing and the routine skull T1T 2 was performed. Results in group A, 4 animals died during the scanning, and the respiration increased gradually. Before PWI, 8 animals in group B received second anesthesia. All the animals in group B were all recovered faster than those in group A. Conclusion in the experiment, the state of the small animals can directly affect the process of the experiment and the quality of the experimental results, but stable. Safe anesthesia is an effective way to control the state of experimental animals. In this study, respiratory anesthesia can better control the breathing of rats, and ensure the safety of animals, thereby improving the quality of the experiment. To provide experimental support for clinical research.
【作者单位】：第三军医大学大坪医院野战外科研究所放射科;
【基金】：国家自然科学基金资助项目(81271626);国家自然科学基金青年基金资助项目(81201139) 重庆市自然科学基金资助项目(cstc2012jjB10028)
【分类号】：R614
【正文快照】： MR检查过程中,检查对象状态的稳定性及配合程度直接学大坪医院野战外科研究所动物实验中心),体质量为(200±影响检查结果[1]。特别是在小动物实验中,麻醉方式的准确选10)g。随机分为A、B两组,A组为注射麻醉组,B组为呼吸麻择[2],严重影响图像的采集与图像的质量,直接影响研究的

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