异氟醚对老年大鼠认知功能及大脑颞叶GABA受体1和NMDA受体2B表达的影响

发布时间：2018-02-07 13:02

本文关键词： 异氟醚 Morris水迷宫 γ-氨基丁酸受体 N-甲基-D-天冬氨酸受体B 颞叶　出处：《临床麻醉学杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的观察吸入不同浓度异氟醚后老年大鼠空间记忆以及脑右侧颞叶γ-氨基丁酸受体1(GABAR1)和N-甲基-D-天冬氨酸受体2B(NMDAR2B)表达的变化。方法雄性SD老年大鼠90只,9月龄,随机分为对照组(C组,n=10)和实验组(n=80)。C组吸入室温空气,实验组按照异氟醚浓度和吸入持续时间分为S1组(1.5%-2h)、S2组(2.5%-2h)、S3组(1.5%-4h)和S4组(2.5%-4h)。各组均分为两组分别在麻醉后1、7d进行Morris水迷宫实验,采用RT-PCR技术和免疫荧光技术检测右侧脑颞叶GABAR1和NMDAR2B的mRNA表达和蛋白含量。结果与对照组比较,异氟醚吸入1d后S1、S2、S3、S4组的空间记忆能力伴随着药物浓度升高和吸入持续时间延长出现持续下降,颞叶中GABAR1的mRNA表达和蛋白含量明显升高,NMDAR2B的mRNA表达和蛋白含量明显降低(P0.01)。异氟醚吸入7d后,S1、S2、S3组大鼠的空间记忆能力与对照组差异无统计学意义,S4组明显低于对照组和其他三组(P0.01);S1、S2、S3组GABAR1和NMDAR2B的mRNA表达和蛋白含量与对照组差异无统计学意义;与对照组比较,S4组GABAR1的mRNA表达和蛋白含量明显升高,NMDAR2B的mRNA表达和蛋白含量明显降低(P0.01)。结论持续吸入异氟醚对空间记忆能力的影响显著,而且长时间高浓度的吸入方式造成的影响时间较长。这种影响与脑颞叶中GABAR1和NMDAR2B等神经递质受体的表达相关。
[Abstract]:Objective to observe the changes of spatial memory and the expression of 纬 -aminobutyric acid receptor (GABAR1) and N-methyl-Daspartate receptor (2BN-NMDAR2B) in the right temporal lobe of aged rats after inhaling different concentrations of isoflurane. Methods 90 male Sprague-Dawley aged rats were aged 9 months. The experimental group was divided into two groups according to the concentration of isoflurane and the duration of inhalation. The experimental group was divided into S1 group and 1.5 -2 h group. The control group and the S4 group were divided into two groups: the control group (n = 1.5-1) and the group S 4 (n = 2.5-4h). Each group was divided into two groups: one day and seven days after anesthesia, the Morris water labyrinth test was carried out on the 1st day and 7th day after anesthesia, and the control group was divided into two groups: the control group, the control group, the control group, the control group, the control group, and the control group, the control group and the experimental group. The mRNA expression and protein content of GABAR1 and NMDAR2B in the right temporal lobe were detected by RT-PCR and immunofluorescence techniques. The spatial memory ability of S1S2S2S3OS4 group was continuously decreased with the increase of drug concentration and the prolongation of inhalation duration after 1 d inhalation of isoflurane. The expression of mRNA and protein content of GABAR1 in temporal lobe were significantly increased. The mRNA expression and protein content of NMDAR2B were significantly decreased in NMDAR2B. The spatial memory ability of rats in the S1S2S3 group was significantly lower than that in the control group after 7 days of inhalation of isoflurane. There was no significant difference between the S4 group and the control group. There was no significant difference in mRNA expression and protein content of GABAR1 and NMDAR2B between the control group and the other three groups. Compared with the control group, the mRNA expression and protein content of GABAR1 in S4 group were significantly increased, and the mRNA expression and protein content of NMDAR2B were significantly decreased (P 0.01). Conclusion the effects of continuous inhalation of isoflurane on spatial memory were significant. The effects of prolonged high concentration inhalation were related to the expression of neurotransmitter receptors such as GABAR1 and NMDAR2B in the temporal lobe of the brain.
【作者单位】：河北北方学院;
【基金】：河北北方学院自然科学研究计划项目(120176)
【分类号】：R614

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