不同推拿手法对食蟹猴轻度颈动脉粥样硬化模型的影响

发布时间：2018-02-08 15:11

本文关键词： 颈动脉粥样硬化斑块内膜损伤拉曼实验压力实验　出处：《中国临床解剖学杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的探讨不同颈椎推拿手法对食蟹猴轻度颈动脉粥样硬化(CAS)模型稳定性的影响。方法将18只食蟹猴建立轻度CAS模型,并以高脂饲料喂养8周,用彩超证实其造模成功。再将18只食蟹猴随机分为3组(每组6只),分别是:颈椎旋转组、推桥弓组和模型对照组。分别对颈椎旋转组和推桥弓组的食蟹猴进行相应的手法干预,共干预30 d,模型对照组不做特殊干预。最后将动物麻醉后处死,取颈总动脉分别进行HE染色、拉曼实验和压力实验。结果 HE染色可见颈总动脉血管内壁损伤严重,有斑块形成。拉曼试验中,3组均有明显的1450及1660脂质特征峰,但三组特征峰的相对强度差异无显著性意义(P0.05)。压力实验中,三组中食蟹猴颈动脉斑块破裂压力检测结果显示无显著性意义(P0.05)。结论在食蟹猴轻度CAS病变中,短期内施用颈椎旋转手法和推桥弓手法并不会改变斑块的稳定性。
[Abstract]:Objective to investigate the effects of cervical massage on neck atherosclerosis mild cynomolgus monkeys (CAS) affect the stability of the model. Methods: 18 cynomolgus monkeys establish mild CAS model, and fed with high fat diet for 8 weeks, with ultrasound confirmed successful modeling. Then 18 cynomolgus monkeys were randomly divided into 3 groups (each group 6), namely: cervical rotation group, Qiaogong group and control group respectively. The corresponding manual intervention on cervical rotation group and cynomolgus monkeys Qiaogong group, a total of 30 d intervention, the model control group did not intervene. The animal were sacrificed after anesthesia, the external carotid artery were stained with HE, Raman and pressure analysis. The results of HE staining showed carotid artery wall damage, plaque. Raman test, the 3 groups were 1450 and 1660 lipid obvious characteristic peaks, but there was no significant difference between the three groups of relative intensity of characteristic peak pressure (P0.05). In the three groups, there was no significant difference in the detection results of carotid plaque rupture pressure between the two groups (P0.05). Conclusion in the mild CAS disease of cynomolgus monkeys, cervical spine rotation and push bridging maneuver in a short time will not change the stability of the plaques.

【作者单位】：西南医科大学附属中医医院;南方医科大学中医药学院;广州中医药大学;
【基金】：四川省教育厅科研项目(17ZB0472) 泸州市科技局科技计划项目(2016-176-13) 西南医科大学-西南医科大学附属中医医院联合专项项目(2016-4-4)
【分类号】：R244.1

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