创伤较小的小鼠主动脉弓缩窄术的建立

发布时间：2018-02-25 03:08

本文关键词： 主动脉弓缩窄左室肥厚压力超负荷小鼠　出处：《中国比较医学杂志》2015年02期 　论文类型：期刊论文

【摘要】：目的探索一种对小鼠创伤较小的主动脉弓缩窄术的手术方法。方法 32只雄性昆明(KM)小鼠,随机分为假手术10只及模型(主动脉弓缩窄术)组22只;模型组动物麻醉后仰卧位固定,行气管插管接通呼吸机。右侧卧位重新固定,左侧第二肋间开胸,分离主动脉弓并用5-0真丝编织线将其套扎在26G注射器针头行缩窄操作,缩窄后退出针头,关胸。假手术组动物同期行开胸,而不进行缩窄操作,其余操作步骤与模型组相同。造模期间及造模后4周记录动物死亡情况;于术后4周对两组小鼠行心脏超声及心脏病理学检测。结果 1模型组总死亡率约为32%。2超声心动图结果:模型组主动脉弓缩窄处血流速率均大于2 400 mm/s;假手术组主动脉弓缩窄处血流速率均小于900 mm/s。与假手术组比较,模型组左室收缩末期内径(LVSD)、左心室收缩期容积(LVSV)、左心室重量(LV mass,LVM)、左心室重量指数(LV mass index,LVMI)明显增加(P0.05);射血分数(EF)、左心室缩短分数(FS)显著降低(P0.05)。3病理切片可见血管扩张及血管壁增厚表现。结论本造模方法能够成功增加心脏后负荷,诱导后负荷增加所致的心肌肥厚病理生理过程。与国内报道较多的劈开胸骨的造模方法相比,该造模方法对模型动物的损伤更小,在与心肌肥厚病理生理机制及相关药效研究中具有很大的应用价值。
[Abstract]:Objective to explore an operative method for coarctation of aortic arch in mice. Methods 32 male Kunming KM mice were randomly divided into sham operation group (n = 10) and model group (n = 22). In the model group, after anesthesia, the animals were fixed in supine position, intubated with ventilator, refixed in the right lateral position, left second intercostal thoracotomy, separated aortic arch and bound to 26G syringe needle with 5-0 silk knitting thread. The animals in the sham-operation group underwent thoracotomy at the same time, and the other procedures were the same as those in the model group. The death of the animals was recorded during the modeling period and 4 weeks after the operation. Results 1 the total mortality of the model group was about 32.2 echocardiography results: the blood flow velocity at the coarctation of aortic arch in the model group was more than 2 400 mm / s; in the sham operation group, the blood flow rate was more than 2 400 mm / s. The blood flow rate in the coarctation of aortic arch was less than 900 mm / s. In model group, left ventricular end-systolic diameter (LVSD), left ventricular systolic volume (LVSVV), left ventricular mass (LVM), left ventricular mass index (LVMI) and left ventricular mass index (LVMI-LV mass) were significantly increased (P 0.05), ejection fraction (EF) and left ventricular shortening fraction (FS) were significantly decreased (P 0.05.3). Conclusion this method can increase cardiac afterload successfully. The pathophysiological process of myocardial hypertrophy caused by the increase of induced afterload. Compared with the more commonly reported methods for the making of sternum in China, the damage of this method to the model animals is less than that of the others. It has great application value in the study of pathophysiological mechanism and related pharmacodynamics of myocardial hypertrophy.
【作者单位】：北京中医药大学基础医学院;
【基金】：国家自然科学基金(81102829) 教育部新世纪优秀人才支持计划(NCET-13-0692)
【分类号】：R654.3

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