不同时间的乳化氟碳保存液处理对供体肺抗炎抗氧化能力及组织结构的影响

发布时间：2018-02-26 09:12

本文关键词： 乳化氟碳保存液(FCE) UW液不同时段肺保护　出处：《暨南大学学报(自然科学与医学版)》2015年04期 　论文类型：期刊论文

【摘要】：目的:观察乳化氟碳保存液(FCE)和UW液处理供体肺在不同时间对肺组织抗炎、抗氧化能力及其结构的影响.方法:选择48只约350~400 g SD雄性大鼠随机分为两组(FCE组与UW组),FCE组与UW组分别又随机各分为两个亚组.对每只大鼠进行麻醉、气管插管与机械通气后,剖胸,全身肝素化后将灌注管自大鼠右心室插入肺动脉,再用保存液进行灌注,灌注完后快速取下心肺组织置入相应的保存液中进行保存.乳化氟碳组(FCE)用乳化氟碳进行灌注与保存,一组保存6 h(FCE-6 h),另一组保存12 h(FCE-12 h).UW液组(UW)用UW液灌注与保存,保存方法及保存时间同FCE组.在相应时段取肺组织,检测白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、丙二醛(MDA)、髓过氧化酶(MPO)、超氧化物歧化酶(SOD)的水平并观察其组织结构变化.结果:(1)两组保存液处理6 h,肺组织MDA、MPO含量及病理积分显著低于12 h组(P0.05),SOD活性则显著高于12 h组(P0.05);(2)FCE组两个时段肺组织MPO含量及病理积分显著低于UW组(P0.05);(3)FCE组在病理改变较轻微,而UW组则较重.结论:与UW液相比,乳化氟碳同样具有抑制炎症,延缓并减轻缺血保存期肺损伤的能力,且乳化氟碳在两个时段的抗氧化能力均强于UW液.
[Abstract]:Objective: to observe the anti-inflammatory effects of emulsified fluorocarbon preservative fluid (FCEE) and UW solution on lung tissue at different time. Methods: 48 male SD rats were randomly divided into two groups: FCE group and UW group. After tracheal intubation and mechanical ventilation, thoracotomy and heparinization of the whole body inserted the perfusion tube into the pulmonary artery from the right ventricle of the rat. After perfusion, the heart and lung tissues were quickly removed and stored in the corresponding preservation solution. The emulsified fluorocarbon group was perfused and preserved with emulsified fluorocarbon, one group was stored for 6 h, the other group was stored for 12 h, the other group was perfused and preserved with UW solution. The method and time of preservation are the same as those of FCE group. The levels of interleukin-1 尾, interleukin-6 (IL-6), tumor necrosis factor- 伪 (TNF- 伪), malondialdehyde (MDAA), myeloperoxidase (MPO) and superoxide dismutase (SOD) were detected and the changes of tissue structure were observed. The content of MPO and the pathological score of lung tissue in 12 h group were significantly lower than those in UW group (P 0.05) and the pathological score was significantly lower than that in UW group (P 0.05) and the pathological score was significantly lower than that in UW group (P 0.05) and the pathological score was significantly lower than that in UW group (P 0.05) and the pathological score was significantly lower than that in UW group. Conclusion: compared with UW solution, emulsified fluorocarbon also has the ability of inhibiting inflammation, delaying and alleviating lung injury during ischemic preservation, and the antioxidant ability of emulsified fluorocarbon in both periods is stronger than that in UW solution.
【作者单位】：暨南大学第一附属医院麻醉科;深圳龙华新区人民医院麻醉科;
【基金】：广东省高科技发展专项基金项目(2060303)
【分类号】：R614

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