不同品系正常成年小鼠听性脑干反应特点

发布时间：2018-02-28 07:36

  本文关键词： 小鼠 短声 短纯音 听性脑干反应　出处：《听力学及言语疾病杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的研究正常成年CBA、C57BL、昆明系和129系四种品系小鼠的听性脑干反应(auditory brainstem response,ABR),分析其ABR反应阈和潜伏期,得到正常值范围,标准化测试流程,为小鼠的听觉相关研究提供参考。方法对6周龄正常CBA、C57BL、昆明系和129系四种品系小鼠各20只(20耳),完全麻醉后,进行短声(click)和短纯音(tone burst,tb)ABR测试,观察四种品系小鼠ABR各波的引出率,记录引出率最高的波反应阈值及各波潜伏期。结果 (1)在80dB SPL强度下,CBA、C57BL、昆明系和129系四种品系小鼠ABR各波引出率均为100%,CBA小鼠ABR波形分化最好;在30dB SPL强度下,除129小鼠外,其余3种小鼠波Ⅱ引出率最高,129小鼠在阈值附近50dB SPL强度下也是波Ⅱ引出率最高;129小鼠ABR反应阈明显高于其它三种小鼠,差异均有统计学意义(P均0.05);(2)四种品系小鼠的短纯音听觉敏感频率为8、12、16kHz,其中对12kHz的短纯音最敏感;(3)麻醉情况下,ABR各波潜伏期随着测试时间延长而延长,尤其波Ⅲ以后各波潜伏期延长更明显。80dB SPL强度下CBA、C57BL、昆明系、129系四种小鼠ABR波形在波Ⅰ前均可分化出SP(summating potential,SP)波,引出率分别为100%、90%、80%、50%,相同刺激条件下,SP波出现率高的小鼠其ABR反应阈相对较低。结论麻醉状态下,CBA、C57BL、昆明系和129系四种品系小鼠短声及短纯音ABR波Ⅱ引出率最高,故以波Ⅱ刚刚出现的刺激声强度确定为反应阈值;C57BL、昆明系和129系三种品系小鼠的ABR结果均没有CBA小鼠稳定,四种品系小鼠中CBA小鼠更适合用来建立听觉功能相关研究的动物模型;SP波可间接反映毛细胞功能。
[Abstract]:Objective to study the auditory brainstem response (ABR) of normal adult CBA C57BL, Kunming strain and 129-strain mice, and to analyze the threshold and latency of ABR response, and to obtain the normal range and standardized test procedure. Methods A total of 20 mice (20 ears from Kunming strain and 129 strain) were anesthetized to test the ABR. The extraction rate of ABR waves in four strains of mice was observed. Results at 80 dB SPL intensity, the highest threshold of wave response and the latency of each wave were recorded in Kunming strain and 129-strain mice, respectively. The extraction rates of ABR waves in Kunming and 129-strain mice were all the best, and at 30 dB SPL intensity, the ABR waveform differentiation was the best in Kunming strain and 129-strain mice, except for 129dB SPL intensity. Under the intensity of 50dB SPL near the threshold, the ABR reaction threshold of the other three kinds of mice was significantly higher than that of the other three kinds of mice, and the ABR reaction threshold of the other three mice was significantly higher than that of the other three kinds of mice. The difference was statistically significant (P < 0.05)) the frequency of short tone auditory sensitivity in four strains of mice was 812kHz and 16kHz, and the latency of ABR waves was prolonged with the prolongation of test time under anesthetized condition. In particular, after wave 鈪，

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