丙泊酚对兔严重烫伤早期并发肾损伤时肾脏的保护作用

发布时间：2018-02-28 12:15

本文关键词： 丙泊酚严重烫伤肾损伤　出处：《华中科技大学学报(医学版)》2017年01期 　论文类型：期刊论文

【摘要】：目的探讨丙泊酚对兔严重烫伤早期并发肾损伤时肾脏的保护作用。方法取18只新西兰兔,构建30%体表总面积深度烫伤模型,随机分为烫伤对照组(S组)和丙泊酚麻醉组(P组)。P组烫伤后1 h开始静脉注射丙泊酚1mg/kg,然后以4 mg/(kg·h)的速率持续泵注3 h。S组以等容量的生理盐水替代丙泊酚。两组动物分别于烫伤前(T_0)、烫伤后4 h(T_1)、烫伤后8 h(T_2)、烫伤后12 h(T_3)、烫伤后24 h(T_4)采集血样,夹心酶联免疫吸附法测定血清TNF-α、IL-1、IL-6浓度;烫伤前、烫伤后6、12、24 h生化法测定尿素氮(BUN)和肌酐(Cr)含量;观察动物烫伤后24 h肾脏组织病理学变化。结果与T_0时点比较,两组T_(1~4)时点血清TNF-α、IL-1和IL-6浓度升高(P0.05或P0.01);P组经过丙泊酚处理后,T_(1~4)时点血清中TNF-α、IL-1和IL-6浓度比S组明显降低(P0.05或P0.01)。烫伤后血清BUN、Cr的含量明显高于烫伤前(P0.05或P0.01);P组烫伤后各时点血清BUN、Cr含量明显低于S组(P0.05或P0.01)。P组较S组肾脏病理学损伤程度减轻。结论丙泊酚对严重烫伤后肾脏功能受损和病理学改变有一定的缓解作用,这种保护机制很可能与丙泊酚抑制严重烫伤时IL-1、IL-6和TNF-α浓度升高相关。
[Abstract]:Objective to investigate the protective effect of propofol on kidney injury in early stage of severe scalding in rabbits. Methods 18 New Zealand rabbits were used to establish a deep scalding model with 30% body surface area. They were randomly divided into scalded control group (S group) and propofol anesthesia group (P group). The propofol 1 mg / kg was injected intravenously 1 h after scalding in propofol anesthesia group, and then the propofol was continuously injected at the rate of 4 mg/(kg 路h). The propofol was replaced by normal saline of the same volume in both groups. Blood samples were collected from animals before scalding, 4 hours after scalding, 8 hours after scalding, 8 hours after scalding, 12 hours after scalding, 12 hours after scalding, 24 hours after scalding. Serum TNF- 伪 IL-1- 6 was determined by sandwich enzyme linked immunosorbent assay (Elisa), bun (bun) and creatinine (Cr) were determined by biochemical method before and after scald for 24 h, and renal histopathological changes were observed at 24 h after scalding. The results were compared with those at T0 time point. The concentration of TNF- 伪 -1 and IL-6 in serum was significantly lower than that in group S (P 0.05 or P 0.01 P 0.05 after scalding). The serum levels of TNF- 伪 -1 and IL-6 were significantly lower than those in group S (P0.05 or P0.01P) at the time point after scalding. The content of BUNCr in serum after scalding was significantly higher than that in the group before scalding or P0.01P (P < 0.05) at the time point of treatment with propofol or propofol, the concentration of TNF- 伪, IL-1 and IL-6 was significantly lower than that of group S (P < 0.05). The level of bun Cr in serum at each time point was significantly lower than that in group S (P 0.05 or P 0.01). Conclusion Propofol can alleviate the damage of renal function and pathological changes after severe scalding. This protective mechanism may be related to the inhibitory effect of propofol on the elevated levels of IL-1 and TNF- 伪 in severe scalded rats.
【作者单位】：武汉市第三医院麻醉科;
【分类号】：R644

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