玫参颗粒对围绝经期大鼠内分泌-免疫功能的影响

发布时间：2018-03-05 09:50

本文选题：玫参颗粒　切入点：围绝经综合征　出处：《中药药理与临床》2015年02期 　论文类型：期刊论文

【摘要】：目的:探讨玫参颗粒治疗妇女更年期综合征的内在机制。方法:大鼠随机分为7组,即假手术组、模型组、坤宝丸阳性对照组、玫参颗粒10g/kg组、玫参颗粒5g/kg组、玫参颗粒2.5g/kg组、戊酸雌二醇片组。除了假手术外,其他各组去除双侧卵巢造模,各给药组给予相应药物灌胃,模型组与假手术组给予蒸馏水灌胃,连续干预30d,腹腔麻醉后腹主动脉取血,测定大鼠血清雌二醇(E2)、卵泡刺激素(FSH)、促黄体生成激素(LH)、超氧化物歧化酶(SOD)和白介素2(IL-2)的水平,处死大鼠后计算大鼠子宫、胸腺、脾脏、肾上腺系数;HE染色观察子宫、阴道组织形态学变化。结果:(1)玫参颗粒5g/kg组、10g/kg组能明显抑制因大鼠去卵巢而造成的体重增加,其中以10g/kg组的抑制幅度有极显著差异。(2)玫参颗粒能显著提高去卵巢大鼠子宫、肾上腺系数,其中10g/kg组提高幅度有极显著差异。(3)玫参颗粒能显著提高去卵巢大鼠胸腺和脾脏系数,其中2.5g/kg组、5g/kg组、10g/kg组提高胸腺系数幅度均有极显著差异;5g/kg组提高脾脏系数有显著性差异,10g/kg组提高脾脏系数有极显著差异。(4)玫参颗粒能提高去卵巢大鼠血清中SOD、IL-2的水平,其中2.5g/kg组、5g/kg组、10g/kg组提高SOD水平幅度有极显著地差异;2.5g/kg组、5g/kg组提高IL-2水平有显著性意义,10g/kg组提高IL-2幅度有极显著差异。(5)玫参颗粒能提高双侧卵巢切除的wistar雌性大鼠血清E2水平,2.5g/kg组提高幅度达显著水平,5g/kg组、10g/kg组提高幅度达极显著水平;玫参颗粒还能显著降低去卵巢大鼠升高的FSH水平和LH水平,2.5g/kg组、5g/kg组、10g/kg组均能极显著降低FSH水平,2.5g/kg组能显著降低去卵巢大鼠LH水平,5g/kg组、10g/kg组能极显著降低去卵巢大鼠LH水平。(6)通过子宫、阴道HE染色发现玫参颗粒能显著改善去卵巢大鼠子宫内径狭小、萎缩的现象,2.5g/kg组、5g/kg组、10g/kg组改善均有显著性差异;玫参颗粒还能改善阴道萎缩症状,2.5g/kg组、5g/kg组、10g/kg组改善均具有显著性差异。结论:玫参颗粒治疗围绝经期综合征与调节机体内分泌-免疫系统有关,为临床应用和新药开发提供实验数。
[Abstract]:Objective: to explore the intrinsic mechanism of Meishen granule in the treatment of menopausal syndrome in women. Methods: rats were randomly divided into 7 groups: sham operation group, model group, Kun Bao pill positive control group, Meishen granule 10 g / kg group, Meishen granule 5 g / kg group, Meishen granule 2.5 g / kg group. Estradiol valerate group: except sham-operation, the other groups removed the bilateral ovarian model, each group was given the corresponding drugs by stomach, the model group and the sham operation group were given distilled water for 30 days, the abdominal aorta blood was taken after abdominal anaesthesia, and the rats in the model group were treated with distilled water for 30 days. The serum levels of estradiol E _ (2), follicle stimulating hormone (FSH), luteinizing hormone (LHH), superoxide dismutase (SOD) and interleukin-2 (IL-2) were measured. The uterine, thymus, spleen and adrenal gland coefficients were observed by HE staining after the rats were killed. Results the weight gain caused by ovariectomized rats was significantly inhibited in 5g / kg Meisen granule group, and the inhibition range of 10g / kg group was very significant difference (P < 0.05). Meishen granule could significantly increase the uterus of ovariectomized rats. The coefficient of adrenal gland in 10 g / kg group was significantly higher than that in 10 g / kg group (P < 0.05). Meisen granule could significantly increase the coefficients of thymus and spleen in ovariectomized rats. There were significant differences in the amplitude of thymus coefficient in 2.5 g / kg group and 5 g / kg group in 10 g / kg group. There were significant differences in the spleen coefficient of 5 g / kg group. The increase of spleen coefficient in 10 g / kg group could increase the level of IL-2 in serum of ovariectomized rats. There was significant difference in the increase of SOD level in 2.5 g / kg group and 5 g / kg group in 10 g / kg group. There was significant difference between 2.5 g / kg group and 5 g / kg group in increasing IL-2 level. There was a significant difference in increasing IL-2 amplitude in 10 g / kg group.) Meishen granule could increase the level of IL-2 in bilateral ovariectomized wistar female rats. The serum E _ 2 level was significantly increased in 2.5 g / kg group, and the increase range was extremely significant in 10 g / kg group of 5 g / kg group. Meishen granule also significantly decreased the elevated FSH level in ovariectomized rats and LH level in 2.5 g / kg group and 10 g / kg group in 5 g / kg group. The FSH level was significantly decreased in 2.5 g / kg group. The LH level in ovariectomized rats was significantly decreased in 5 g / kg group and 10 g / kg group in 10 g / kg group. Rat LH level. 6) through the uterus, Vagina HE staining showed that Meishen granules could significantly improve the narrow uterine diameter and atrophy in the uterus of ovariectomized rats. There were significant differences in the improvement of uterine diameter and atrophy in the 2.5 g / kg group and the 5 g / kg group (10 g / kg group). Meisen granules can also improve the symptoms of vaginal atrophy. There are significant differences in the improvement of the symptoms of vaginal atrophy in the 5 g / kg group and 10 g / kg group. Conclusion: the treatment of peri-menopausal syndrome by Meisen granule is related to regulating the endocrine and immune system of the body. To provide experimental data for clinical application and new drug development.
【作者单位】：兰州大学基础医学院中西医结合研究所;南方医科大学中医药学院;北京中医药大学基础医学院;
【基金】：创新中药玫参颗粒的临床前研究,广东省高等学校人才引进项目
【分类号】：R285.5

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