臭氧化盐水静脉注射对大鼠切口痛镇痛效果及血浆TNF-α、IL-6的影响研究

发布时间：2018-03-08 05:32

本文选题：臭氧化盐水　切入点：大鼠切口痛　出处：《中南大学》2014年硕士论文　论文类型：学位论文

【摘要】：摘要目的：观察不同浓度臭氧化盐水静脉注射对大鼠切口痛模型行为学及血浆TNF-α、IL-6水平的影响,探讨静脉应用臭氧化盐水对大鼠急性疼痛的镇痛效果、合理治疗浓度、不良反应及其可能机制。方法：健康成年雄性SD大鼠54只,随机选取24只用于疼痛行为学测试,30只用于酶联免疫吸附(ELISA)法检测大鼠血浆TNF-α和IL-6浓度。其中,用于疼痛行为学测试的24只大鼠随机分为4组,每组6只,A、B、C、D组分别于造模前10min经尾静脉给药,A组给氧气化盐水,B、C、D组分别给浓度20μg/m1.3μg/m1.40μg/ml的臭氧化盐水,四组均给药5ml/kg,七氟烷麻醉后按Brennan法制作切口痛模型,分别在术前24h、术后2、6、24、48h五个时间点测试并记录大鼠50%机械缩足阈值和累积疼痛评分。用于血浆TNF-α和IL-6浓度检测的30只大鼠随机分为5组,每组6只,A、B、C、D组处理同前,E组为假手术组,吸入七氟烷之后不做处理,术后2h用10%水合氯醛腹腔麻醉后,快速打开胸腔,右心室取血2ml,用ELISA法检测大鼠血浆TNF-α和IL-6水平。同时,观察记录每只静脉注射臭氧化盐水的大鼠的不良反应。结果：(1)不同浓度臭氧化盐水静脉注射后,均未发现大鼠血管炎、死亡的不良事件。 (2)术前24h各组大鼠50%PWMT(g)比较无明显差别(P0.05),术后2h、6h、48h各组大鼠50%PWMT有显著差异(P0.05),A组与B组比较在各时间点50%PWMT差异均无统计学意义(P0.05),而A组与C、D两组比较在术后2、6、24、48h50%PWMT明显减小,差异均有统计学意义(P0.05),C组与D组在各时间点50%PWMT差异均无统计学意义(P0.05)。 (3)术前24h各组大鼠CPS均为0,术后各时间点各组大鼠CPS均有显著差异(P0.01),且差异随时间逐渐变小。A组在术后不同时点CPS分别较B、C、D组高,均有显著差异(P0.01),且·D组与A组差异最大,C组次之。 (4)术后2h各组大鼠血浆TNF-α浓度有显著差异(P0.001)。两两比较,E组与B、D两组差异无统计学意义(P0.05),A组和C组较E组血浆TNF-α浓度均升高,其中C组升高较A组明显。 (5)术后2h各组大鼠血浆IL-6浓度有显著差异(P0.001)。两两比较,E组与A组差异无统计学意义(P0.05),B、C、D组较E组血浆IL-6浓度均升高,其中D组最高,B组最低。结论：(1)20-40μg/ml的臭氧化盐水静脉注射对大鼠切口痛有镇痛效果。 (2)其镇痛效果与臭氧化盐水浓度存在剂量依赖性,浓度越高镇痛效果越强。 (3)30μg/ml的臭氧化盐水静脉注射会使大鼠血浆中TNF-α水平升高,20-40μg/ml的均使血浆IL-6水平升高。 (4)20～40μg/ml的臭氧化盐水静脉注射对大鼠无明显不良反应。
[Abstract]:Abstract. Objective: to observe the effect of different concentrations of ozonated saline on the behavior of rat incision pain model and the level of plasma TNF- 伪 IL-6, and to explore the analgesic effect and reasonable therapeutic concentration of ozonated saline on acute pain in rats. Adverse reactions and their possible mechanisms. Methods: Twenty-four male Sprague-Dawley (SD) rats were randomly selected to measure plasma TNF- 伪 and IL-6 by enzyme linked immunosorbent assay (Elisa). Twenty-four rats were randomly divided into 4 groups. Six rats in each group were given oxygen vaporized brine (OGSCC) (20 渭 g / m 1.3 渭 g 路m ~ (1.40) 渭 g / ml) in group A (n = 10), respectively. All the four groups were given 5 ml / kg sevoflurane to make incision pain model according to Brennan method. The threshold of mechanical contraction and the cumulative pain score of 50% rats were measured and recorded at 24 hours before operation and 24 hours after operation. 30 rats who were used to detect plasma TNF- 伪 and IL-6 concentration were randomly divided into 5 groups. After inhaling sevoflurane, the rats were anesthetized intraperitoneally with 10% chloral hydrate at 2 hours after operation. The thoracic cavity was quickly opened and blood was taken from the right ventricle at 2 ml. The plasma levels of TNF- 伪 and IL-6 were measured by ELISA method. At the same time, Adverse reactions were recorded in each rat intravenously injected with ozonated saline. Results (1) there were no adverse events of vasculitis and death in rats after intravenous injection of different concentrations of ozonated saline. (2) there was no significant difference in 50 PWMTg between the two groups 24 hours before operation (P 0.05), but there was no significant difference in 50 PWMT between group A and group B at 2 h to 48 h after operation. There was no significant difference in 50 PWMT between group A and group B at each time point, but the PWMT of group A and group C decreased significantly at 2 hours after operation, 24 minutes and 48 hours after operation, compared with group B, there was no significant difference between group A and group C in terms of PWMT (P 0.05), and there was no significant difference between group A and group C (P 0.05), and there was no significant difference between group A and group C (P 0.05). There was no significant difference in PWMT between group C and group D at each time point. (3) the CPS of each group was 0 24 hours before operation. There was significant difference in CPS of each group at each time point after operation. The difference gradually decreased with time. The CPS of group A was higher than that of group D at different time points after operation. There was significant difference between group D and group A (P 0.01), and group 路D had the highest difference between group A and group C. (4) there was a significant difference in plasma TNF- 伪 concentration between two groups at 2 h after operation (P 0.001). There was no significant difference between group E and group B (P 0.05) and group C (P 0.05) and group C (P < 0.05). The plasma TNF- 伪 level in group C was significantly higher than that in group A (P < 0.05), and that in group C was significantly higher than that in group A (P < 0.05). (5) there was significant difference in plasma IL-6 concentration between two groups at 2 h after operation (P 0.001). There was no significant difference between E group and A group. The plasma IL-6 concentration in group D was higher than that in group E, and the highest in group D was the lowest in group B. Conclusion 20-40 渭 g / ml of 20-40 渭 g / ml of ozonated saline has analgesic effect on incision pain in rats. (2) the analgesic effect is dose-dependent with the concentration of ozonated saline, and the higher the concentration is, the stronger the analgesic effect is. 30 渭 g / ml ozonated saline intravenously increased the level of TNF- 伪 in plasma of rats, and 20-40 渭 g / ml of TNF- 伪 increased the level of IL-6 in plasma of rats. 40 渭 g / ml of 40 渭 g / ml ozonated saline had no significant adverse effects on rats.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R614

【参考文献】