改良经大鼠胃十二指肠动脉逆行肝动脉插管试验方法的研究

发布时间：2018-03-10 06:18

本文选题：胃十二指肠动脉　切入点：肝动脉　出处：《贵州医科大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:通过建立一种改良的经大鼠胃十二指肠动脉逆行肝动脉插管的新方法,探讨其在临床实验研究中运用的可行性及价值。方法:选取成年健康清洁级Wistar大鼠120只,随机平均分为A、B、C、D组,每组30只;其中A组与B组为预实验组,分别采用改良法与传统法行肝动脉插管。对于C、D组研究对象,采用穿刺种植法建立大鼠肝癌模型,分别以改良法与传统法插管行肝动脉造影及介入化学栓塞治疗,栓塞材料均选择碘油,比较两种方法的插管成功率、大鼠肝癌建模的成功率、肿瘤体积变化、各组大鼠死亡原因及实验死亡率。结果:A组插管成功率90.0%(27/30),B组插管成功率63.3%(19/30),A组插管成功率明显高于B组(?2=4.565,P=0.033)。对C、D组研究对象进行肿瘤种植建立大鼠肝癌模型,C与D两组间肿瘤种植成功率为100%。待肝癌模型制作成功后,术前扫描C、D组大鼠肝左叶均可见大小不一的团块状低密度影,边缘较清晰,未见腹水及肝脏子灶形成。经过A、B组预实验后,C组插管成功率93.3%(28/30),D组插管成功率70.0%(21/30),死亡原因可能为血管受损、肝脏损伤及麻醉意外等。C组插管成功率明显高于D组(?2=4.007,P=0.045)。而C组与D组介入栓塞治疗前后平均肿瘤体积无统计学差异(P0.05),但D组大鼠术后10天死亡率较C组明显升高(42.86%vs 10.71%,?2=5.079,P=0.024)。结论:改良经胃十二指肠动脉插管造影肝动脉方法简单、成功率高,术后死亡率低、并发症少;值得继续深入研究和的相关实验中进行尝试,可进一步加强动物实验的深度和介入治疗基础研究的广度,值得继续深入研究和的相关实验中进行尝试。
[Abstract]:Objective: to establish a new method of retrograde hepatic artery catheterization through gastroduodenal artery in rats and to explore the feasibility and value of its application in clinical experiment. Methods: 120 healthy adult Wistar rats were selected. Group A and group B were randomly divided into two groups: group A and group B were pretreated with hepatic artery catheterization by modified method and traditional method respectively. The liver cancer model of rats was established by puncture and implantation method for the patients in group C and D, which were divided into two groups: group A (n = 30), group B (n = 30), group B (n = 30), group B (n = 30). Hepatic arteriography and interventional chemoembolization were performed by the improved method and the traditional method respectively. Lipiodol was used as the embolization material. The success rate of intubation in the two methods, the success rate of rat liver cancer modeling, and the change of tumor volume were compared. Results the success rate of intubation in group B was significantly higher than that in group B (n = 63.3) and the success rate of intubation in group A was significantly higher than that in group B. The success rate of tumor implantation between group C and group D was 100. When the model of liver cancer was made successfully, the left lobes of liver in group C and D could be seen in the left lobe of rats of different sizes. The success rate of intubation in group C was 93.3%. The success rate of intubation in group D was 70.021 / 30. The cause of death might be vascular injury, liver injury and anaesthesia accident. The success rate of intubation in group C was significantly higher than that in group D. There was no significant difference in mean tumor volume between group C and group D before and after embolization, but the mortality rate of group D was significantly higher than that of group C (42.86 vs 10.71) 10 days after embolization. Conclusion: the improved gastroduodenal artery catheterization is simple, with high success rate, low postoperative mortality and less complications. It can further strengthen the depth of animal experiments and the breadth of basic research of interventional therapy.
【学位授予单位】：贵州医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.7

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