同步辐射类同轴成像技术在大鼠肝脏小叶结构微血管显微成像中的应用

发布时间：2018-03-18 23:12

本文选题：同步辐射　切入点：类同轴成像技术　出处：《复旦学报(医学版)》2015年01期 　论文类型：期刊论文

【摘要】：目的利用同步辐射类同轴X线相位衬度成像技术对大鼠肝脏样本进行成像研究,旨在评价类同轴相衬成像技术在肝脏细微结构成像方面的意义。方法随机将6只C57BL大鼠分为两组,一组麻醉后直接开腹取肝脏标本,另一组麻醉后经肠系膜上静脉插管,注入生理盐水置换肝脏血管内血液,结扎肝脏血管及胆管后取出肝脏标本,经4%甲醛溶液固定后在上海光源X射线成像及生物医学应用光束站线BL13W1上进行成像,并与病理图片比较,随后对两组血管成像图像质量进行评分,测量管径并进行统计学分析。结果使用空间分辨率为0.37μm的电荷耦合感光元件(charge coupled device,CCD),注入生理盐水置换肝脏血管内血液并经干燥后,同步辐射类同轴技术能清晰显示大鼠肝脏的肝小叶结构,能分辨肝小叶中央静脉、肝板、肝窦间隙等显微解剖结构。结论同步辐射类同轴相衬成像技术能清晰显示大鼠肝脏血管及肝小叶微细解剖结构,对软组织脏器成像具有较大的应用价值,在肝纤维化及肝肿瘤早期血管成像研究上具有很大的潜力。
[Abstract]:Objective to study the phase contrast imaging of rat liver using synchrotron radiation coaxial X-ray phase contrast imaging technique. Methods six C57BL rats were randomly divided into two groups. Normal saline was injected to replace the intravascular blood of the liver. After ligation of the hepatic vessels and bile ducts, the liver samples were taken out. After fixed with 4% formaldehyde solution, the liver samples were imaged on the X-ray imaging of Shanghai light source and the BL13W1 of the beam line of biomedical application beam station. And then compared with the pathological images, the quality of the two groups of angiographic images was evaluated. Results the charge coupled deviceCCDA was used to replace the intravascular blood of the liver with a spatial resolution of 0.37 渭 m. Synchrotron radiation coaxial technique can clearly display the hepatic lobule structure of the rat liver, and can distinguish the central hepatic vein and the hepatic plate. Conclusion Synchrotron radiation coaxial phase contrast imaging technique can clearly display the microanatomy of hepatic vessels and lobules in rats, and has great application value in soft tissue visceral imaging. It has great potential in the study of liver fibrosis and early vascular imaging of liver tumors.
【作者单位】：复旦大学附属中山医院放射科;上海市影像医学研究所;复旦大学上海医学院影像学系;上海交通大学附属瑞金医院放射科;同济大学附属上海市第十人民医院放射科;中科院上海应用物理研究所;
【基金】：国家自然科学基金(81371542,81071154)~~
【分类号】：R575.2

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