气管内滴入与腹膜腔注射脂多糖致肺组织炎症损伤的动态变化比较

发布时间：2018-03-21 04:04

本文选题：急性肺损伤　切入点：动物模型　出处：《中国呼吸与危重监护杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的比较气管内滴入脂多糖与腹膜腔注射脂多糖两种不同方法复制的急性肺损伤小鼠模型的生理、病理特点及动态变化。方法 BALB/c小鼠麻醉后分别经气管内滴入脂多糖(5 mg/kg)和经腹膜腔内注射脂多糖(5 mg/kg)。在脂多糖处理后第1、2、6、12、18、24、48 h处死小鼠,通过检测支气管肺泡灌洗液(BALF)蛋白含量、肺湿/干重比值和肺组织病理半定量评分评价呼吸系统损伤程度,ELISA法检测血清与BALF上清中肿瘤坏死因子-α(TNF-α)浓度以了解全身与肺局部炎症反应程度。结果两组脂多糖处理小鼠的BALF中蛋白含量、肺湿/干重比值、血清/BALF中TNF-α浓度均增高。腹膜腔注射脂多糖小鼠肺湿/干重比值较气管滴入脂多糖小鼠高,其余上述指标在两组间无显著差异。两组小鼠在脂多糖处理后肺组织炎症细胞浸润、肺泡间隔增宽、肺组织病理半定量评分均明显增高,以气管内滴入组病理损伤较显著。结论气管内滴入脂多糖与腹腔注射脂多糖均可引起肺局部炎症与组织损伤,两种模型病理损伤程度差异较大,自身好转趋势不同,应根据实验目的选择合适的模型复制方法。
[Abstract]:Objective to compare the physiology of acute lung injury mice induced by intratracheal instillation of lipopolysaccharide and peritoneal injection of lipopolysaccharide. Methods BALB/c mice were treated with lipopolysaccharide (LPS) 5 mg / kg and intraperitoneal lipopolysaccharide 5 mg / kg respectively after anesthesia. The mice were killed at 48 h after lipopolysaccharide treatment. The content of BALF protein in bronchoalveolar lavage fluid (BALF) was measured. Lung wet / dry weight ratio and lung pathology semi-quantitative evaluation of the degree of respiratory injury; Elisa method was used to detect TNF- 伪 TNF- 伪 in serum and BALF supernatant to understand the degree of systemic and regional inflammation. Protein content in BALF of Sugar treated mice, The lung wet / dry weight ratio and TNF- 伪 concentration in serum / BALF were higher in peritoneal injection lipopolysaccharide mice than in trachea instilled lipopolysaccharide mice. There was no significant difference between the two groups in the other indexes mentioned above. After lipopolysaccharide treatment, inflammatory cells infiltrated the lung tissue, the alveolar septum widened, and the semiquantitative score of lung histopathology increased significantly in the two groups. Conclusion both intratracheal lipopolysaccharide and intraperitoneal injection of lipopolysaccharide can cause lung inflammation and tissue injury. The appropriate model replication method should be selected according to the purpose of the experiment.
【作者单位】：广州军区广州总医院呼吸内科;解放军第458医院呼吸内科;
【基金】：国家自然科学基金面上项目(81370173);国家自然科学基金青年科学基金(8120002,81200025) 广东省医学科学技术研究基金(A2016305) 广东省科技计划项目(2016ZC0213)
【分类号】：R563.8

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