降逆和胃汤对胆汁反流性胃炎模型大鼠的影响

发布时间：2018-03-21 05:56

  本文选题：降逆和胃汤　切入点：胆汁反流性胃炎　出处：《辽宁中医药大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：本实验是采用反流液来复制大鼠胆汁反流性胃炎动物模型，在造模成功后对其进行药物治疗，通过降逆和胃汤对反流性胃炎大鼠动物模型一般状态，血清白介素-6（LI-6）、肿瘤坏死因子（TNF-a）、胃泌素（GAS）、内皮素（ET）水平，胃黏膜组织病理学改变，并与铝碳酸镁片进行对比，阐明降逆和胃汤防治大鼠胆汁反流性胃炎胃黏膜损伤的作用机制。 材料与方法： 1分组造模与干预治疗 健康雌雄Wister实验大鼠共72只，在实验室自然采光下，适应性喂养3天。根据大鼠的体重不同，将符合条件的大鼠随机分为了6组：空白组、模型组、降逆和胃汤高、中、低剂量组及西药阳性对照组，按大鼠性别雌雄各半。除空白组给予等量蒸馏水灌胃外，其余5组大鼠均连续向胃内灌反流液进行造模15d，反流液配制以胰酶1．5g、牛磺胆酸钠2．5g、孵磷脂0．25g(奥博星)三种溶于100ml的蒸溜水之中，并以1．5ml／lOOg灌胃，日2次。造模结束以后，模型组和空白组继续灌胃同样给予等容量的生理盐水，西药阳性对照组予铝碳酸镁片。降逆和胃汤高、中、低组剂量分别为37.2g、18.6、9.3生药/kg体重。 2一般状况的观察 在实验过程中，对大鼠的精神状态、饮食、二便情况及反流液灌胃后体重、毛色和死亡等情况变化进行详细的记录观察。 3实验指标取材及检测 灌胃30天后各组大鼠进行取材:最后一次灌胃结束，禁食禁水12小时。取材当天对大鼠先进行称重，按体重向腹腔内注射麻醉，每只腹主动脉取血2ml，用做血清白介素-6、肿瘤坏死因子-a、胃泌素、内皮素水平的测定。取血完毕，无菌操作下取胃。用剪刀沿胃的大弯处剪开，胃中内容物拿冰生理盐水洗清，再用滤纸将胃组织吸干，，将其放入多聚甲醛溶液里面以固定，进行电子显微镜下观察胃黏膜的病理学改变。 结果： 第一部分：降逆和胃汤对胆汁反流性胃炎模型大鼠血清中LI-6、TNF-a、GAS、ET-2含量的影响 各组大鼠血清IL-6、GAS、TNF-a、ET含量的比较。与模型组相比较,降逆和胃汤高、中、低剂量组与西药阳性对照组均可提高IL-6、GAS、TNF-a，降低ET的含量，均具有统计学意义（P0．05)，与西药阳性对照组相比较，和胃降逆汤高剂量组IL-6、TNF-a、GAS、ET未有明显变化：差异无统计学意义(P0．05)。 第二部分：降逆和胃汤对胆汁反流性胃炎模型大鼠胃黏膜病理学上的影响空白组中的大鼠胃黏膜呈现出光滑、无脱离及破损，很少能看到炎症细胞的出现。模型组中的大鼠胃黏膜则出现脱离和破损，可见大量的炎症细胞。在与模型组的比较中，降逆和胃汤高、中、低剂量各组和西药阳性对照组胃黏膜细胞的脱离和破损比较轻，炎症细胞的浸润比较少，降逆和胃汤高剂量组效果最佳，其次是中剂量和西药阳性对照组。对半定量指标所显示出的炎症细胞结果：较模型组，降逆和胃汤高、中、低剂量各组与西药阳性对照组中的炎症细胞浸润明显减轻（P0.05）。 结论： 1降逆和胃汤对胆汁反流性胃炎大鼠的白细胞介素-6、肿瘤坏死因子-a、胃泌素含量显著的升高，内皮素含量降低。加强了胃黏膜的保护作用，减少对胃黏膜的损伤。 2在治疗胆汁反流性胃炎用降逆和胃汤，可减少炎症细胞的浸润，能改善胆汁反流性胃炎模型大鼠胃黏膜病理学的变化，拥有保护胃黏膜，减少胃黏膜损伤的作用。
[Abstract]:Objective: the purpose of this experiment is the use of reflux liquid in rats with bile reflux gastritis animal model, the drug therapy in after the success of the model, through Jiangni Hewei decoction on reflux gastritis rat animal model of general condition, serum interleukin -6 (LI-6), tumor necrosis factor (TNF-a), gastrin (GAS), endothelin (ET) levels, pathological changes of gastric mucosa pathology, and compared with Hydrotalcite Tablets, and clarify Jiangni Decoction in Treating Gastric Bile Reflux Gastritis in rats with gastric mucosal injury mechanism.
Materials and methods:
1 group modeling and intervention therapy
Healthy male Wister rats 72 only, in the natural lighting laboratory, adaptive feeding for 3 days. According to the body weight of rats, the rats were randomly divided in order to meet the conditions of the 6 groups: blank group, model group, jiangnihewei dose, and low dose group and positive medicine control group, according to the rat sex female. In addition to the control group given distilled water by gavage, the remaining 5 rats were intragastric perfusion to continuous reflux liquid molding 15d, reflux liquid prepared by sodium taurocholate trypsin 1.5g, 2.5G, 0.25g (aoboxing) with phospholipid three dissolved in 100ml distilled water, and the 1.5ml / lOOg orally, 2 times a day. After the end of the modeling, model group and blank group to gavage also received the equal volume of normal saline control group, positive medicine to Hydrotalcite Tablets. Jiangnihewei dose, low dose group, respectively 37.2g, 18.6,9.3 crude drug /kg weight.
2 observation of the general situation
During the experiment, the changes of the mental state, diet, two urine, weight, hair color and death of the reflux fluid were recorded and observed in detail.
3 experimental indexes and testing
30 days after the gavage rats were collected: the last gavage end, fasting for 12 hours. Based on the day of the rats were weighed according to the weight to the intraperitoneal injection of anesthesia, each abdominal aortic blood 2ml, with serum interleukin -6, tumor necrosis factor -a, gastrin, determination of endothelial on level of blood. After aseptic operation the stomach. The stomach big bend along with scissors cut, with ice saline wash contents in the stomach, and the stomach tissue paper dry, put it into the poly Formaldehyde Solution inside a fixed, the pathological changes of gastric mucosa was observed by electron microscope.
Result锛

本文编号：1642548