细胞外信号蛋白调节激酶5在异氟醚后处理减轻大鼠海马脑片缺氧无糖损伤中的作用

发布时间：2018-03-21 06:45

本文选题：麻醉药　切入点：异氟醚后处理　出处：《中国现代医学杂志》2017年21期 　论文类型：期刊论文

【摘要】：目的探讨异氟醚后处理大鼠海马脑片缺氧无糖(OGD)损伤神经保护机制中细胞外信号蛋白调节激酶5(ERK5)的作用。方法使用雄性SD大鼠,将其麻醉后取出海马组织,制成离体脑片,然后随机进行正常对照组(Con组)、损伤组(OGD组)和药物处理组的处理。采用花粉活力染色(TTC)法评价各组脑片损伤程度,采用碘化丙啶(PI)染色法检测海马CA1区神经细胞凋亡程度,采用实时荧光定量聚合酶链反应(q RT-PCR)测定细胞外调节蛋白激酶信使核糖核酸(ERK5 m RNA)的表达,采用Western blot法检测ERK5蛋白的表达与磷酸化水平。结果与Con组比较,OGD组脑片损伤程度升高、海马CA1区凋亡细胞增多,海马组织内ERK5 m RNA和p-ERK5表达升高(P0.05);XMD组阻断ERK5 m RNA和p-ERK5表达,脑片损伤程度升高、海马CA1区凋亡细胞增多(P0.05)。与OGD、1.5%ISPOC和4.5%ISPOC组比较,3.0%ISPOC组脑片损伤程度减轻、海马CA1区凋亡细胞减少,海马组织内ER K5 m R N A和p-ER K5表达升高(P0.05)。与3.0%ISPO C组比较,XMD阻断ISPOC海马组织内ERK5 m RNA和p-ERK5表达,脑片损伤程度升高、海马CA1区凋亡细胞增多(P0.05)。结论一定浓度的异氟醚后处理大鼠海马脑片对抗O GD损伤的保护性机制可能与上调ER K5 m R N A和ERK5磷酸化水平有关。
[Abstract]:Objective to investigate the role of extracellular signal protein regulated kinase (ERK5) in the neuroprotective mechanism of hypoxia-free OGD in rat hippocampal slices treated with isoflurane. Methods male SD rats were anesthetized and the hippocampal tissue was taken out and made into isolated brain slices. Then the normal control group (Con group), the injured group (OGD group) and the drug treated group were randomly treated. The degree of injury of brain slices was evaluated by pollen vigor staining method, and the degree of neuronal apoptosis in the hippocampal CA1 area was detected by the method of pyridine iodide staining. The expression of ERK5 mRNA in extracellular regulated protein kinase messenger ribonucleic acid (ERK5) was detected by real-time fluorescence quantitative polymerase chain reaction (RT-PCR). Results compared with Con group, ERK5 protein expression and phosphorylation level were detected by Western blot. Results compared with Con group, the degree of brain slice damage and apoptosis in the CA1 area of hippocampus were increased, and the expression of ERK5 m RNA and p-ERK5 in hippocampus was increased. The expression of ERK5 m RNA and p-ERK5 was blocked in the Con group. The degree of brain slice injury was increased, and the apoptotic cells in hippocampal CA1 area were increased. Compared with OGD1.5 and 4.5 ISPOC groups, the degree of brain slice damage was reduced and the apoptotic cells in hippocampal CA1 area decreased. The expression of ERK5 m RNA and p-ERK5 increased in hippocampus. Compared with 3.0 ISPO C group, XMD blocked the expression of ERK5 m RNA and p-ERK5 in ISPOC hippocampus. Conclusion the protective mechanism of hippocampal slices treated with isoflurane may be related to the up-regulation of ERK5 mRNA and ERK5 phosphorylation.
【作者单位】：石河子大学医学院第一附属医院麻醉科;石河子大学医学院生理教研室;
【基金】：国家自然科学基金(No:81360203)
【分类号】：R614

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