沙棘黄酮对粥样硬化大鼠抗氧化及NADPH氧化酶亚基的影响

发布时间：2018-03-23 20:03

本文选题：动脉粥样硬化　切入点：沙棘黄酮　出处：《青海大学》2014年硕士论文

【摘要】：目的：通过复制大鼠动脉粥样硬化病理模型，探讨沙棘黄酮(TFH)对动脉粥样硬化(AS)大鼠血三脂和LDL-C、主动脉病理改变及SOD、MDA的影响，并测定动脉粥样硬化大鼠血管中NADPH氧化酶亚基NOX4和P47phox蛋白表达含量，探讨沙棘黄酮保护动脉粥样硬化大鼠血管的可能的分子机制。方法：采用50只健康清洁级、雄性SD鼠，随机分为5组：正常对照组，动脉粥样硬化组和沙棘黄酮低、中、高剂量组。动脉粥样硬化组和沙棘黄酮不同剂量组采用高脂饲料并腹腔注射维生素D3的方法来复制大鼠粥样硬化模型，正常对照组则继续饲喂普通饲料，沙棘黄酮不同剂量组自造模开始后每日分别以沙棘黄酮50mg/kg、100mg/kg、200mg/kg定量喂食。8周后，在大鼠禁食12小时后麻醉大鼠，从腹主动脉插管提纯血清后采用全自动生化分析仪测定血三脂和LDL-C含量,比色法测定SOD及MDA含量；处死大鼠后采用主动脉HE染色来观察主动脉病理形态学变化，余主动脉采用Elisa方法检测其NADPH氧化酶亚单位NOX4和P47phox蛋白的含量变化。结果：与正常对照组相比动脉粥样硬化组大鼠血脂表达紊乱，与动脉粥样硬化组比较，沙棘黄酮不同剂量组HDL-C的含量显著升高，TG、TC、LDL-C等含量明显减少，变化有统计学意义(P0.05)。与动脉粥样硬化组比较，沙棘黄酮不同剂量组SOD的表达含量明显升高，MDA的表达显著下降，变化有统计学意义(P0.05)。HE染色示动脉粥样硬化大鼠血管内皮损伤，沙棘黄酮不同剂量组内皮损伤有所缓解，脂质沉积较少。与正常对照组比较，动脉粥样硬化组大鼠P47phox和NOX4蛋白表达含量升高，与动脉粥样硬化组比较，沙棘黄酮不同剂量组P47phox和NOX4蛋白表达显著降低，差异有统计学意义(P0.01)。结论：沙棘黄酮具有调节脂质代谢，提高机体防御氧化应激能力，阻止大鼠动脉粥样硬化进展，，其机制可能与降低NADPH氧化酶蛋白表达有关。
[Abstract]:Objective: to replicate the model of atherosclerosis in rats, to investigate the Seabuckthorn flavonoids (TFH) on atherosclerosis (AS) rats blood lipid and LDL-C in three, aortic pathological changes and SOD, MDA, and vascular atherosclerosis rats NADPH oxidase subunit NOX4 and P47phox protein expression, investigate the possible molecular mechanism of flavonoids of seabuckthorn the protection of vascular atherosclerosis rats.
Methods: 50 healthy clean grade male SD rats, were randomly divided into 5 groups: normal control group, atherosclerosis group and seabuckthorn flavonoids, low and high dose group. Atherosclerosis group and different dose groups of seabuckthorn flavonoids using method of high fat diet and intraperitoneal injection of vitamin D3 to copy the model of atherosclerosis in rats, normal the control group was fed with normal diet to different dose groups, seabuckthorn flavonoids from the model after the start of day respectively by 100mg/kg, 200mg/kg 50mg/kg of seabuckthorn flavonoids, quantitative feeding after.8 weeks, the rats in the rats were fasted for 12 hours after anesthesia, purification of serum from abdominal aorta after intubation blood were measured by automatic biochemical analyzer and LDL-C three fat content determination of SOD, MDA and the content of colorimetry; rats were sacrificed after the aortic HE staining to observe pathological changes in the aorta, the aorta was detected by Elisa more than the NADPH oxidase subunit NOX The changes in the content of 4 and P47phox proteins.
Results: compared with normal control group the expression of atherosclerosis rats blood lipid disorder, compared with atherosclerosis group, the content of seabuckthorn flavonoids in different dosage group HDL-C significantly increased, TG, TC, LDL-C decreased significantly, with statistically significant difference (P0.05). Compared with the atherosclerosis group, the expression content of seabuckthorn flavonoids in different dosage group SOD the increased expression of MDA decreased significantly, with statistically significant difference (P0.05).HE staining showed atherosclerotic vascular endothelial injury in rats, the sea buckthorn flavone different dose groups of endothelial injury eased, lipid deposition is less. Compared with normal control group, atherosclerosis group, P47phox and NOX4 protein expression in rats was increased, compared with atherosclerosis group. The expression of P47phox and NOX4 protein in different dose groups of seabuckthorn flavonoids decreased significantly, the difference was statistically significant (P0.01).
Conclusion: Hippophae rhamnoides flavonoids can regulate lipid metabolism, improve the body's ability to prevent oxidative stress, and prevent progression of atherosclerosis in rats, which may be related to the reduction of NADPH oxidase protein expression.

【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R543.5

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