急性宫颈扩张对脊髓TRPA1表达的影响

发布时间：2018-03-24 01:30

本文选题：宫颈扩张　切入点：瞬时受体电位通道蛋白A　出处：《广东医学》2017年03期

【摘要】：目的探讨急性宫颈扩张(UCD)后胸腰段脊髓瞬时受体电位通道蛋白(TRPA1)表达的变化。方法成年雌性大鼠60只,随机分为5组:假手术组和UCD25、UCD50、UCD75、UCD100组。全凭吸入麻醉下,除S组外,各组大鼠分别采用25、50、75、100 g的砝码进行持续10 s的宫颈扩张,重复1次,间隔5 min;假手术组不作扩张。记录UCD时大鼠腹直肌肌电图及心率。待UCD结束后10 min处死大鼠,分别运用实时荧光定量PCR及Western blot法测定脊髓胸腰段TRPA1的变化。结果与假手术组相比,接受不同张力UCD的各组大鼠腹直肌肌电活动及胸腰段脊髓TRPA1表达均明显增加(P0.05)。组间两两比较:与UCD25组或UCD50组相比,UCD75组和UCD100组肌电图活动及脊髓TRPA1表达明显增加,差异有统计学意义(P0.05);但UCD25组与UCD50组间以及UCD75组与UCD100组之间差异无统计学意义(P0.05);而且急性UCD后胸腰段脊髓TRPA1的表达变化与UCD对腹直肌肌电图的影响趋于一致。结论胸腰段脊髓TRPA1可能参与了急性UCD相关内脏痛的形成。
[Abstract]:Objective to investigate the changes of transient receptor channel protein (TRPA1) expression in thoracolumbar spinal cord after acute cervical dilatation. Methods 60 adult female rats were randomly divided into 5 groups: sham operation group and UCD25 UCD50 UCD75 UCD100 group. Rats in each group were treated with 2550 / 75100g weight for 10 s of cervical dilatation, repeated once at intervals of 5 mins, and no dilatation was made in sham-operation group. Electromyography and heart rate of rectus abdominis were recorded during UCD. The rats were killed 10 min after the end of UCD. The changes of TRPA1 in thoracolumbar segment of spinal cord were measured by real-time fluorescence quantitative PCR and Western blot. The myoelectric activity of rectus abdominis and the expression of TRPA1 in thoracolumbar spinal cord were significantly increased in rats receiving different tension UCD. Compared with UCD25 group or UCD50 group, EMG activity and TRPA1 expression in spinal cord of UCD75 group and UCD100 group were significantly increased. There was no significant difference between UCD25 group and UCD50 group and between UCD75 group and UCD100 group, and the change of TRPA1 expression in thoracolumbar spinal cord after acute UCD was consistent with the effect of UCD on rectus abdominis electromyography. TRPA1 of thoracolumbar spinal cord may be involved in the formation of visceral pain associated with acute UCD.
【作者单位】：南昌大学第二附属医院麻醉科;
【基金】：江西省卫生计生委科技计划(编号:20155239)
【分类号】：R714.3

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