抗戾解毒汤体内抑制小鼠高致病性禽流感病毒H5N1增殖

发布时间：2018-03-24 14:10

本文选题：抗戾解毒汤　切入点：HN　出处：《中国老年学杂志》2015年06期

【摘要】：目的探讨抗戾解毒汤体内抑制高致病性禽流感病毒H5N1增殖的效果。方法小鼠麻醉后使用高致病性禽流感病毒H5N1的鼠适应株滴鼻以感染,感染后120 min内,实验组给予抗戾解毒汤灌胃,奥司他韦组给予奥司他韦溶液腹腔注射,对照组给予PBS缓冲液灌胃,之后观察体重变化和死亡率,并通过实时PCR方法检测肺部病毒负载水平。结果实验组存活率(80%)较奥司他韦组(53.3%)和对照组(3.3%)显著提高(P0.01),实验组体重下降也低于其他两组;实验组肺部病毒负载水平较对照组显著降低(P0.01),和奥司他韦组无明显差异(P0.05)。结论抗戾解毒汤对感染H5N1病毒的小鼠的病毒增殖抑制作用明显,且保护作用强,可以作为流感病毒的备选或者辅助药物。
[Abstract]:Objective to investigate the effect of Kangsurijiedu decoction on inhibiting the proliferation of highly pathogenic avian influenza virus (H5N1) in vivo. Methods mice using highly pathogenic avian influenza virus H5N1 after anesthesia were infective by nasal drip, within 120 min after infection. The experimental group was given Kangsurijiedu decoction, the oseltamivir group was given oseltamivir solution intraperitoneally, the control group was given PBS buffer, then the weight change and mortality were observed. Results the survival rate of the experimental group was significantly higher than that of the oseltamivir group (53.3%) and the control group (3.31%), and the weight loss of the experimental group was lower than that of the other two groups. Compared with the control group, the pulmonary virus loading level in the experimental group was significantly lower than that in the control group, and there was no significant difference between the two groups. Conclusion Kangsurijiedu decoction can inhibit the virus proliferation in mice infected with H5N1 virus and has a strong protective effect. It can be used as an alternative or auxiliary drug for influenza viruses.
【作者单位】：广州医科大学基础学院医学遗传与细胞生物学教研室;中山大学附属第三医院产科实验室;
【基金】：国家自然科学青年基金(81102715) 广东省自然科学基金(A2010219) 广州市科技计划项目(2012J4100012) 广州市教育局科研课题(2012C132)
【分类号】：R285.5

【共引文献】