两种糖皮质激素导致骨质疏松过程中骨量、骨转换标志物及雌激素水平的差异

发布时间：2018-03-24 18:36

本文选题：糖皮质激素　切入点：骨质疏松　出处：《中国骨质疏松杂志》2015年02期

【摘要】：目的对比泼尼松龙(PRE)和地塞米松(DXM)致骨质疏松过程中对大鼠骨量和骨转换标志物、雌激素水平的差异。方法选取3月龄SPF级雌性大鼠46只,随机分成4组:基线组(BL组)6只、年龄对照组(AM组)12只、泼尼松龙组(PRE组)14只、地塞米松组(DXM组)14只。BL组于实验开始时麻醉处死,其余各组分别予常规饲养,PRE组以5mg/kg PRE每天一次皮下注射,DXM组以1mg/kg DXM每周两次皮下注射,于干预后1、2、3个月(M1、M2、M3)分3批麻醉处死取材。每次取材时立即取子宫、肾上腺称重,并收集血清以检测血清内雌激素、PINP及β-CTX水平、收集腰1-3椎体以检测腰椎骨密度(BMD)。结果 PRE组各时间点BMD值[(0.183±0.027、0.230±0.005、0.259±0.014)g/cm2]及DXM组各时间点BMD值[(0.191±0.010、0.208±0.012、0.200±0.004)g/cm2]均较AM组[(0.251±0.014、0.275±0.009、0.281±0.008)g/cm2]明显下降(P0.05),其中DXM组下降更为显著(P0.01),且在M2及M3,DXM组BMD值明显低于PRE组(M2:P0.05;M3:P0.01)。PRE组干预初期,其血清雌激素水平(36.54±20.40μg/L)较AM组(148.74±40.33μg/L)明显降低(P0.01),但随着干预时间延长,M3时(130.85±18.95μg/L)增长至与AM组(126.64±69.12μg/L)相接近水平(P0.05),但在DXM干预下,雌激素水平在各时间点[(93.13±31.27、91.77±33.14、98.83±10.58)μg/L]均低于AM组[(148.74±40.33、140.01±28.46、126.64±69.12)μg/L](P0.05)。两种GC干预下,PINP及β-CTX均显著高于AM组(P0.01),且PRE干预后各时间点PINP水平[(1410.33±882.40、2089.23±1623.61、1546.88±644.68)μg/L]显著高于DEX组[(258.70±139.42、220.89±92.82、483.36±225.82)μg/L](P0.05)。结论地塞米松诱导骨量减少的能力明显强于泼尼松龙,这可能与其更大程度地降低血清雌激素水平以及更有效地限制成骨有关。
[Abstract]:Objective to compare the effects of prednisolone (pre) and dexamethasone (DXM) on bone mass, bone turnover markers and estrogen levels in rats. Methods 46 SPF grade female rats aged 3 months were randomly divided into 4 groups: baseline group (BL group, n = 6) and control group (n = 6). 12 rats in the age control group, 14 rats in the prednisolone group and 14 rats in the DXM group and 14 rats in the dexamethasone group were anesthetized and killed at the beginning of the experiment. The other groups were given subcutaneous injection of 5mg/kg PRE once a day with 1mg/kg DXM twice a week respectively in the other groups. The rats in the control group were killed in 3 batches of anaesthesia at 1 and 3 months after intervention. The uterus and adrenal gland were taken immediately at each time of sampling, and the adrenal gland was weighed. Serum samples were collected to detect the levels of PINP and 尾 -CTX in serum. Results the BMD value of PRE group at different time points [0.183 卤0.027 卤0.230 卤0.005 卤0.259 卤0.014)g/cm2] and the BMD value of DXM group [0.191 卤0.010 卤0.208 卤0.012 卤0.200 卤0.004)g/cm2] were significantly lower than those of AM group [0.251 卤0.0140.275 卤0.0090.281 卤0.008)g/cm2], and the BMD value of DXM group was significantly lower than that of AM group [0.251 卤0.0140.275 卤0.0090.281 卤0.008)g/cm2], and the BMD value in M2 and M3DXM group was significantly lower than that in AM group (0.251 卤0.0140.275 卤0.0090.281 卤0.008)g/cm2). In PRE group, M2: P0.05and M3: P0.01. The serum estrogen level (36.54 卤20.40 渭 g / L) was significantly lower than that of AM group (148.74 卤40.33 渭 g / L), but it increased to 126.64 卤69.12 渭 g / L with the prolongation of intervention time (130.85 卤18.95 渭 g / L). The level of estrogen in AM group [93.13 卤31.27 卤91.77 卤33.142.40 98.83 卤10.58 渭 g / L] was significantly lower than that in AM group [148.74 卤40.33146.64 卤69.126.64 卤69.126.64 卤69.126.64 卤69.126.64 卤69.126.64 卤69.126.64 渭 g / L] at each time point. The levels of PINP and 尾 -CTX were significantly higher than those of AM group at each time point after PRE intervention [1410.33 卤882.400.2023 卤1623.611546.88 卤644.68 渭 g / L] compared with DEX group [258.70 卤139.42220.89 卤92.883.36 卤225.82g / L] P0.050.Conclusion the ability of inducing dexamine to reduce the amount of dexamine is reduced. Better than prednisone, This may be associated with greater reduction of serum estrogen levels and more effective restriction of osteogenesis.
【作者单位】：广州中医药大学第一附属医院二骨科;广州中医药大学;广州军区广州总医院博士后科研工作站;
【基金】：广东省教育厅学科建设专项基金(育苗工程)[2013LYM-0012] 广州中医药大学优秀青年学者科研基金项目(KAB110133K04)
【分类号】：R580

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