电针对脑出血后咳嗽反射重塑的动物实验研究

发布时间：2018-03-25 21:51

本文选题：电针　切入点：脑出血　出处：《黑龙江中医药大学》2017年硕士论文

【摘要】：实验目的:本实验意在通过建立动物模型,说明电项针对脑出血后咳嗽反射机制的重塑作用。实验方法:取雄性SPF级豚鼠45只,体重300±15g之间。适应性饲养一周后,用枸橼酸雾化引咳法给予雾化引咳,分别记录其10分钟咳嗽次数,将10分钟内咳嗽次数少于20次或大于100次的豚鼠剔除,剔除过后补充豚鼠至45只(补充豚鼠也要进行雾化并不满足剔除标准),将经过筛选的45只豚鼠随机分成三组,每组15只,在将每一组随机分成三个小组,每个小组5只样本。分组后进行造模,方法如下:取雄性SPF级豚鼠,体重300±15g之间。实验室适应性饲养一周后将待造模样本用7%水合氯醛(0.5毫升/百克)腹腔注射麻醉,确认麻醉剂生效后将待造模样本以俯卧固定于立体定位仪上,从头部正中开皮,暴露颅骨,在颅骨正中线左侧5mm,冠状缝或前钻一直径1mm的小孔,立体定向穿刺后,用注射器向左侧基底节内(靶点:前囟左侧4-5mm,冠状缝向前0.2mm,进针深度6-7mm,即基底节区),将未肝素化的豚鼠自体血0.03ml缓慢推进基底节区,推注后留针片刻,后缓慢出针。术后局部喷洒庆大霉素,缝合头皮,局部皮肤采用碘伏消毒。之后空白对照组不给予任何治疗,针刺组给予针刺双侧风池、翳风穴,选取针具规格为0.35×25mm,进针深度4mm,针刺过程中行针两次,每次间隔10分钟,用平补平泻手法,每次持续30秒,留针20分钟,每日针刺一次。电针组给予同侧风池、翳风穴连接电针,风池穴为正极,翳风穴为负极,输出波为疏密波,频率以样本头部微颤为标准,输出电流为0.3毫安,留针20分钟,每天治疗一次。后在七天、十四天、二十八天三个时间节点参照LongaFz五级评分法评价样本动物行为学,记录样本10分钟咳嗽次数后取样,按要求测定样本血清白细胞介素4,并在第二十八天取样本肺组织切片,观察样本肺部组织结构变化。实验结果:在相同时间节点比较样本10分钟咳嗽次数,针刺组及电针组与空白对照组比较差异性显著(p0.05),具有统计学意义。同样,对样本行为学评分、血清白细胞介素等方面针刺组及电针组与空白对照组比较差异亦性显著(P0.05),具有统计学意义。实验结论:1.豚鼠脑出血造模后,咳嗽反射明显减弱,且随时间推移可缓慢恢复。2.与对照组比较,针刺与电针均可重塑样本咳嗽反射。3.分析各项实验指标及组织样本,说明电针组效果优于针刺组。
[Abstract]:Objective: the purpose of this experiment was to establish an animal model to explain the effect of electrical item on the remodeling of cough reflex mechanism after intracerebral hemorrhage. Methods: 45 male SPF guinea pigs weighing between 300 卤15g were selected and fed adaptively for one week. The cough was induced by atomization of citric acid, and the coughing times of 10 minutes were recorded respectively. The guinea pigs with less than 20 coughs or more in 10 minutes were removed. After culling, the guinea pigs were added to 45 guinea pigs (the addition of aerosols did not meet the elimination criteria. 45 guinea pigs were randomly divided into three groups with 15 guinea pigs in each group, and each group was randomly divided into three groups. Five samples of each group were divided into groups. The following methods were used: male SPF guinea pigs, weighing between 300 卤15g, were anesthetized by intraperitoneal injection of 7% chloral hydrate (0.5ml / 100g) after a week of adaptive feeding in the laboratory. Confirm that after the anesthetic comes into effect, the shape to be made is fixed on the stereotactic locator with a prone position, the skin is opened from the middle of the head, the skull is exposed, the left side of the midline of the skull is 5mm, the coronal suture or the anterior hole of a diameter 1mm is drilled, and after the stereotactic puncture, Using a syringe to the left basal ganglia (target: anterior fontanelle 4-5mm, coronal suture 0.2mm forward, needle depth 6-7mm, basal ganglia region), the 0.03ml of the unheparinized guinea pig autologous blood was slowly pushed forward to the basal ganglia area, and the needle was kept for a moment. After the operation, gentamicin was sprayed locally, the scalp was sutured, and the local skin was disinfected with iodophor. After that, the blank control group was not given any treatment, and the acupuncture group was given acupuncture at the bilateral Fengchi and Yifeng points. The needle gauge is 0.35 脳 25mm, and the depth of the needle is 4mm. During the acupuncture process, the needle is made twice, with 10 minutes interval, with a flat tonifying and reducing technique, each time lasting 30 seconds, keeping the needle for 20 minutes and needling once a day. The electroacupuncture group is given the same side wind pond and the Yifeng point is connected to the electroacupuncture, The Fengchi point is positive, the Yifeng point is negative, the output wave is a dense wave, the frequency is based on the sample head microtremor, the output current is 0.3 Ma, the needle is kept for 20 minutes and treated once a day. After that, it is treated once a day for seven days and fourteen days. Three time points of 28 days were used to evaluate the animal behavior of the samples according to the LongaFz five-grade scoring method. The samples were sampled after 10 minutes of coughing, and the serum interleukin-4 was measured according to the requirements, and the lung tissue sections were sampled on the 28 day. Observe the changes of lung tissue structure in the sample. Results: at the same time node comparison sample 10 minutes cough times, acupuncture group and electroacupuncture group compared with the blank control group significant difference (p 0.05). The difference between acupuncture group and electroacupuncture group and blank control group was also significant (P 0.05). Conclusion: 1. After making model of guinea pig cerebral hemorrhage, cough reflex was obviously decreased. Compared with the control group, acupuncture and electroacupuncture could reshape the cough reflex of the sample. 3. Analysis of the experimental indexes and tissue samples showed that the effect of the electroacupuncture group was better than that of the acupuncture group.
【学位授予单位】：黑龙江中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245

【相似文献】