乳化七氟烷选择性麻醉新西兰大白兔脑、脊髓模型的建立

发布时间：2018-03-28 07:43

本文选题：麻醉药机制　切入点：七氟烷　出处：《四川大学学报(医学版)》2016年05期

【摘要】：目的建立选择性麻醉脊髓和脑的动物模型,探索七氟烷松弛骨骼肌作用的位点是否在脊髓。方法选择健康新西兰大白兔16只,通过体外循环技术,于第12胸椎(T12)~第1腰椎(L1)水平结扎主动脉,从而形成上半身及下半身两个相对独立的循环系统。通过七氟烷经肺或者氧合器给予到新西兰大白兔上半身或者下半身,实现选择性脑麻醉或者选择性脊髓麻醉(主要是腰骶段脊髓)。连续监测呼气末(代表脑组织)和氧合器出气口(代表脊髓)的七氟烷浓度。利用气相色谱仪,注射器两次平衡法来测量颈动脉(代表脑组织)和腹主动脉(代表脊髓)血液中七氟烷的质量浓度和分压。结果经肺吸入1.5倍肺泡气最低有效浓度(MAC)七氟烷并达平衡状态时,呼气末七氟烷浓度高于氧合器出气口,颈动脉血液中七氟烷的质量浓度和分压高于腹主动脉(P0.05),即脑组织中的七氟烷浓度和分压高于脊髓。经过10~20min洗脱期后,由氧合器给予新西兰大白兔下半身1.5 MAC七氟烷并达平衡状态时,上述指标的表现则完全相反(P0.05),即脊髓中的七氟烷浓度和分压高于脑组织。结论通过七氟烷的不同给予方式(经肺或者氧合器),成功建立了七氟烷选择性麻醉新西兰大白兔脑、脊髓模型。
[Abstract]:Objective to establish an animal model of selective anesthesia of spinal cord and brain and to explore whether the site of sevoflurane relaxation of skeletal muscle is in spinal cord. Methods Sixteen healthy New Zealand white rabbits were selected by cardiopulmonary bypass (CPB). The aorta was ligated horizontally at the 12th thoracic vertebrae T12 ~ 1 L1) to form two relatively independent circulatory systems, the upper and lower half of the body, which were given to the upper or lower half of the New Zealand white rabbit via sevoflurane through the lung or oxygenator. Selective cerebral anesthesia or selective spinal anesthesia (mainly lumbosacral spinal cord). Continuous monitoring of sevoflurane concentrations at end expiratory (representing brain tissue) and oxygenator outlet (on behalf of spinal cord). Gas chromatograph, The mass concentration and partial pressure of sevoflurane in the blood of carotid artery (representing brain tissue) and abdominal aorta (representing spinal cord) were measured by the twice balance method of syringe. Results when inhaling 1.5 times the lowest effective concentration of alveolar gas through the lung and reaching the equilibrium state of sevoflurane, The concentration of sevoflurane at the end of expiratory period was higher than that in the outlet of oxygenator, and the mass concentration and partial pressure of sevoflurane in carotid blood were higher than that in abdominal aorta, that is, the concentration and partial pressure of sevoflurane in brain tissue were higher than those in spinal cord. When a New Zealand white rabbit was given 1.5 MAC sevoflurane in its lower body by oxygenator, On the contrary, the sevoflurane concentration and partial pressure in spinal cord were higher than that in brain tissue. Conclusion the sevoflurane selective anesthesia of the brain of New Zealand white rabbits was successfully established by different ways of giving sevoflurane (via lung or oxygenator). Spinal cord model.
【作者单位】：四川大学华西医院麻醉科;四川大学华西第二医院麻醉科;四川大学华西医院疼痛科;
【基金】：国家自然科学基金(No.81200865)资助
【分类号】：R614

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