骨碎补总黄酮对去势大鼠影响的实验研究

发布时间：2018-03-28 21:05

本文选题：骨碎补总黄酮　切入点：PMOP　出处：《河南中医学院》2014年硕士论文

【摘要】：目的：随着社会科技的进步，特别是医学临床技术和科研力量的提高，人类整体寿命延长，世界上大多数国家老年人人数增多，绝经后骨质疏松症发病率逐年的快速增长，已经成为影响老年人身体健康的主要疾病之一，同时也带来了沉重的社会负担。本研究以骨碎补总黄酮为研究对象，以去势SD大鼠为模型载体，模拟人类雌激素分泌减少后身体机能，观测骨碎补总黄酮对去势大鼠的生化指标、骨密度及病理组织切片、骨组织中端粒酶的表达的影响，探讨骨碎补总黄酮在治疗绝经后骨质疏松症的进程中可能的作用机制。方法：雌性SD大鼠60只，90天龄，体重控制在220—240g，随机分成6组，分别为假手术组（SHAM）、模型组（OVX）、骨碎补总黄酮小剂量组（G小）、骨碎补总黄酮中剂量组（G中）、骨碎补总黄酮大剂量组（G大）、雌二醇（补佳乐）组(E2)各10只，造摸用背侧双切口卵巢摘除术；用蒸馏水或者配制药物溶液灌胃3个月，经麻醉后腹主动脉取血，截取双侧股骨。血液分离取血清测生化指标血清钙（Ca）、磷（P）、骨碱性磷酸酶（B-ALP）；右股骨端用于骨密度测定，再经HE染色，在光镜下观察病理组织学切片；左股骨端测定端粒酶在骨组织中的表达情况。结果：在本实验研究中，与SHAM相比，OVX生化指标检测、骨密度及端粒酶表达值差异有显著统计学意义（P 0.01），G高、E2无统计学意义（P0.05）；与OVX组相比，用药各组血钙、血磷、含量升高，骨碱性磷酸酶降低，其中G高及E2显著统计学意义（P0.01），，G低及G中有统计学意义（P0.05），G高与E2相比，无统计学意义（P0.05）；病理组织切片观察到骨小梁假手术组粗细均匀，分布稠密，可见各种骨细胞，G高及E2变化不大，G低及G中可见骨小梁变细，不均匀，以及边缘锯齿样改变，OVX可见骨小梁断裂、溶解以及融合的脂肪空泡等典型绝经后骨质疏松症改变。结论：骨碎补总黄酮能提高去势大鼠端粒酶的表达量，表明其可以影响骨组织中端粒酶的表达，从而改善绝经后骨质疏松症。
[Abstract]:Objective: with the progress of social science and technology, especially the improvement of medical clinical technology and scientific research strength, the whole life span of human being has been prolonged, the number of elderly people in most countries in the world has increased, and the incidence of postmenopausal osteoporosis has been increasing rapidly year by year. It has become one of the major diseases affecting the health of the elderly, and it also brings a heavy social burden. In this study, the total flavonoids of Fructus fragmentae were used as the research object and the ovariectomized SD rats as the model carrier. The effects of total flavonoids on the biochemical indexes, bone mineral density (BMD) and the expression of telomerase in bone tissue of ovariectomized rats were observed. To explore the possible mechanism of total flavonoids in the treatment of postmenopausal osteoporosis. Methods: sixty female Sprague-Dawley rats (90 days old) were divided into 6 groups randomly and their body weight was controlled at 220-240 g. There were 10 rats in the model group, 10 in the small dose group of flavonoids, 10 in the middle dose group, 10 in the large dose group, 10 in the estradiol group, 10 in the middle dose group, 10 in the total flavonoid group, 10 in the sham-operated group, 10 in the model group, 10 in the small dose group, 10 in the middle dose group, 10 in the large dose group and 10 in the estradiol group, respectively. Ovariectomies were performed with bilateral dorsal incision, and the abdominal aorta was taken after anesthesia for 3 months by gavage with distilled water or prepared drug solution. Bilateral femurs were cut off. Blood samples were taken to measure serum biochemical indexes, such as calcium, phosphophosphatase, bone alkaline phosphatase B-ALP, right femur for bone mineral density determination, HE staining, and histopathological sections under light microscope. The expression of telomerase in bone tissue was measured at the left femur. Results: compared with SHAM, there were significant differences in bone mineral density (BMD) and telomerase expression between the two groups. The level of bone alkaline phosphatase (BALP) was significantly lower than that of E2, and there was no significant difference between P0.05G and E2. The histopathological sections showed that the bone trabecular sham-operated group was uniform in thickness and dense in distribution. It can be seen that the changes of G and E _ 2 in all kinds of bone cells are not obvious, and the trabeculae become thinner and uneven in G, and the bone trabeculae break down, the adipose vacuoles dissolved and fused, and so on. The changes of bone trabeculae in OVX are typical postmenopausal osteoporosis. Conclusion: total flavonoids can increase the expression of telomerase in ovariectomized rats, indicating that it can affect the expression of telomerase in bone tissue and improve postmenopausal osteoporosis.
【学位授予单位】：河南中医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R285.5

【参考文献】