七氟烷吸入麻醉对糖尿病相关认知降低老年大鼠海马Glu及GABA含量的影响

发布时间：2018-03-31 13:55

本文选题：七氟烷　切入点：糖尿病　出处：《福建医科大学》2014年硕士论文

【摘要】：目的探讨七氟烷不同吸入麻醉时间对糖尿病相关认知降低(Diabetes-associated cognitive decline,DACD)老年大鼠认知功能的影响,并从海马递质谷氨酸(Glutamic acid,Glu)及γ-氨基丁酸(Gamma aminobutyric acid,GABA)含量及Glu"GABA比值的变化初步探讨其可能的作用机制,为临床上DACD老年患者合理选用麻醉药物和麻醉方法提供参考。方法选择90只清洁级SD老年大鼠,随机分为3组(n=30):对照组(C组):采用普通饲料饲养8周后,腹腔注射柠檬酸缓冲液,继续原普通饲料饲养7周,期间自由饮水;糖尿病组(D组):采用高糖高脂饲料饲养8周后,腹腔注射柠檬酸缓冲液稀释的链脲佐菌素(Streptozotocin,STZ)后继续原高糖高脂饲养方案7周,期间自由饮水,建立DACD老年大鼠模型;七氟烷组(DS组):先以D组方案构建DACD模型,然后接受1.5%七氟烷吸入麻醉,按时间长短分成3个亚组:DS1组(1小时,n=10)、DS2组(2小时,n=10)、DS3组(3小时,n=10);C组和D组则在同等条件下采用纯氧吸入,根据吸入纯氧时间的不同,分别将C组分为3个亚组:C1(1小时,n=10)、C2(2小时,n=10)、C3(3小时,n=10);D组分为3个亚组:D1(1小时,n=10)、D2(2小时,n=10)、D3组(3小时,n=10)。待七氟烷吸入麻醉或单纯吸入纯氧处理后1天、5天,再次测定C组,D组、DS组各亚组大鼠的认知功能水平,并与处理前进行对比,判断其认知功能的改变。而后采用断头取脑法提取大鼠海马CA1区组织,测定海马CA1区中Glu与GABA含量及Glu/GABA比值变化。结果1、采用高糖高脂喂养复合少量STZ腹腔注射法成功建立T2DM老年大鼠模型;2、处理前认知水平比较,D组各亚组、DS组各亚组均较C组各亚组降低,差异有统计学意义(P0.05);处理后1天认知水平比较,DS3组均较D3、C3降低,差异有统计学意义(P0.05);3、处理后1天比较,DS3组Glu含量降低、GABA含量升高、Glu/GABA比值降低,分别与C3、D3组比较差异均有统计学意义(P0.05)。结论DACD老年大鼠采用1.5%七氟烷吸入麻醉超过3小时及以上,短期内可出现认知功能降低,其作用机制与降低海马CA1区中Glu含量,同时增加GABA含量有关。
[Abstract]:Objective to investigate the effects of different inhalation anesthetic time of sevoflurane on the cognitive function of Diabetes-associated cognitive declination in aged rats. The possible mechanism of Glutamic acid (Glutamic acid Glua), Gamma aminobutyric acidine GABA (纬 -aminobutyric acid) and the ratio of Glu "GABA" in hippocampus were also discussed. Methods 90 old SD rats of clean grade were randomly divided into 3 groups: control group C: normal diet for 8 weeks. Citric acid buffer solution was injected intraperitoneally and continued to be fed with normal diet for 7 weeks, during which drinking water was free. Group D of diabetes mellitus was fed with high-sugar and high-fat diet for 8 weeks. After intraperitoneal injection of streptozotocin (STZ) diluted with citric acid buffer solution, we continued the original high-sugar and high-fat feeding scheme for 7 weeks, during which the aged DACD rat model was established by drinking water freely, while in the sevoflurane group, the DACD model was established in the DS group: group D was used to construct the DACD model. They were then anesthetized with 1.5% sevoflurane and were divided into three subgroups according to the length of time: one hour or one hour, one hour, one hour, one hour, one hour, one hour, one hour, one hour, one hour, one hour, one hour, three hours of pure oxygen inhalation in group C and group D, according to the time of pure oxygen inhalation. Group C was divided into 3 subgroups: 1 h, 1 hour, 1 hour, 1 hour, 1 hour, 1 hour, 1 hour, 1 hour, 1 hour, 1 hour, 10 hour, 10 hour, 3 hours, respectively. Group D was divided into 3 subgroups. Group D was divided into 3 subgroups. Group D was divided into 3 subgroups. After inhaling sevoflurane anesthesia or inhaling pure oxygen for 1 day and 5 days after treatment, group D was divided into three subgroups: group D 1: 1 and group D 2: 1 h after inhalation of sevoflurane or inhalation of pure oxygen alone, the rats were treated with sevoflurane or inhaled pure oxygen for 1 day and 5 days after treatment. The cognitive function of rats in group C and group D were measured again, and compared with those before treatment, the changes of cognitive function were judged. Then the CA1 tissue of hippocampus was extracted by the method of head cut. The content of Glu and GABA and the ratio of Glu/GABA to Glu were measured in the CA1 region of hippocampus. Results 1 the senile rat model of T2DM was successfully established by high glucose and high fat feeding combined with a small amount of STZ intraperitoneal injection. The cognitive level before treatment was compared between the D group and the DS group. Group C was lower than group C. The cognitive level of DS3 group was lower than that of D3C3 group on the first day after treatment, the difference was statistically significant (P 0.05), and the Glu content of DS3 group was lower than that of D3GA-GABA group on the 1st day after treatment, the ratio of Glu-P / GABA to Glu-P / GABA ratio in DS3 group was higher than that in DS3 group. Conclusion the cognitive function of aged DACD rats after inhalation anesthesia of 1.5% sevoflurane for more than 3 hours could be decreased in a short period of time, and its mechanism might be related to the decrease of Glu content in the CA1 area of hippocampus. At the same time, the content of GABA was increased.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R614

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