复合心肌补片对小鼠梗死心肌的修复效果观察

发布时间：2018-04-01 12:01

本文选题：心肌梗死　切入点：小鼠　出处：《山东医药》2015年14期

【摘要】：目的探讨以人心脏瓣膜组织脱细胞基质作为支架的复合骨髓c-kit+干细胞心肌补片(以下称复合心肌补片)对小鼠梗死心肌的修复效果。方法制作复合心肌补片。采用结扎左冠状动脉前降支法制作30只心肌梗死小鼠模型并随机分为复合补片组、补片组及对照组各10只。造模成功3 d时各组小鼠予异氟烷麻醉、固定,开胸暴露心脏。复合补片组将复合心肌补片移植于梗死心肌周边;补片组采用同样方法将脱细胞基质移植于梗死心肌周边;对照组不采取修复措施。采用心脏超声检测移植4周时三组心移植左室射血分数、左室短轴缩短率、心梗面积。结果移植4周时,复合补片组左室射血分数、左室短轴缩短率明显高于补片组及对照组,心梗面积明显小于补片组及对照组,P均0.05。结论复合心肌补片用于修复梗死心肌效果满意。
[Abstract]:Objective to investigate the effect of composite bone marrow c-kit stem cell myocardial patch (hereinafter referred to as composite myocardial patch) with human heart valve tissue acellular matrix as scaffold on the repair of infarct myocardium in mice. Methods compound myocardial patch was prepared. Thirty mice models of myocardial infarction were made by ligating the anterior descending branch of left coronary artery and were randomly divided into two groups. The mice in each group were anesthetized with isoflurane for 3 days, fixed, and the heart was exposed to open chest. In the compound patch group, the compound myocardial patch was transplanted around the infarct myocardium. In the patch group, the acellular matrix was transplanted around the infarct myocardium in the same way, while the control group was not repaired. The left ventricular ejection fraction (LVEF) and the shortening rate of the short axis of the left ventricle were measured by echocardiography in the three groups at 4 weeks after transplantation. Results at 4 weeks after transplantation, the left ventricular ejection fraction (LVEF) and the shortening rate of left ventricular short axis in composite patch group were significantly higher than those in the patch group and control group. The area of myocardial infarction was significantly smaller than that of the patch group and the control group (P 0.05). Conclusion the composite myocardial patch is effective in repairing infarct myocardium.
【作者单位】：上海交通大学医学院附属仁济医院Med-X临床干细胞研究中心;
【基金】：国家自然科学基金资助项目(81370321)
【分类号】：R542.22

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