健脾清化方对非酒精性脂肪肝大鼠内FFA、TG的影响

发布时间：2018-04-01 15:42

本文选题：非酒精性脂肪肝　切入点：健脾清化方　出处：《河南中医学院》2014年硕士论文

【摘要】：研究背景及目的：近年来，非酒精性脂肪性肝病（non-alcoholic fatty liverdisease， NAFLD）已成为一种影响我国人民健康的常见病。在肝细胞内以甘油三酯为主的脂质大量的堆积是本病的特征。人体内中性脂肪分解产生的游离脂肪酸（free fatty acid，FFA）经由肝脏摄取，在肝细胞内参与脂质的合成，各种原因引起的FFA含量增多，造成肝细胞内脂质过度沉积，从而引发NAFLD。本实验通过观察健脾清化方对NAFLD大鼠血清游离脂肪酸（free fatty acid，FFA），肝内甘油三酯（triglyceride，TG）的影响，研究健脾清化方“健脾清热化湿”治法改善脂代谢、治疗脂肪肝的确切疗效。方法：选用健康雄性SD大鼠50只，适应性喂养一周后，采用随机数字表。随机抽取8只为正常组，，给予基础饲料，饮用自来水；其余为高脂组，参考文献[1]给予高脂饲料（84%普通饲料+10%猪油+5%蛋黄粉+1%胆固醇），饮用自来水。10周后，随机从造模组和正常组各抽取2只，用水合氯醛麻醉，处死，取肝脏做病理切片，验证造模成功。再用随机数字表法将高脂组分为模型对照组、健脾清化方高、低剂量组，东宝肝泰组，正常组和模型组灌服生理盐水，各组共给药8周。除正常组外，各组继续以高脂饲料喂养。第18周处死各组大鼠，方法为隔夜禁食不禁水，次日称量体重后，以水合氯醛腹腔注射麻醉，从腹主动脉采血，测定血清谷丙转氨酶（alanine aminotransferase，ALT）、谷草转氨酶（abstract syntax tree，AST），取肝组织，称重，制备肝匀浆及肝组织切片，检测肝组织FFA、TG含量，光镜下观察肝组织病理变化。结果：通过测定各组大鼠给药后肝组织FFA、TG含量的变化，结果表明模型组大鼠肝脏FFA、TG均显著高于空白组（P0.05,）；健脾清化方高、低剂量组及东宝肝泰组对于降低NAFLD大鼠肝内游离脂肪酸(FFA)、TG水平与模型组相比，均有显著差异（P0.05）；健脾清化方高、低剂量组与东宝肝泰组FFA、TG无显著差异（P0.05）结论：1.健脾清化方可以降低NAFLD模型大鼠肝组织中FFA、TG浓度，减轻肝细胞脂变程度； 2.健脾清化方可以降低NAFLD模型大鼠血清ALT、AST水平。说明健脾清化方有保肝降酶作用。
[Abstract]:Background and purpose: in recent years, Non-alcoholic fatty liver disease (NAFLDD) has become a common disease affecting the health of Chinese people. The accumulation of triglycerides in liver cells is the characteristic of the disease. Free fatty acido FFAs were taken through the liver. Taking part in the synthesis of lipids in hepatocytes, the content of FFA increased in various reasons, resulting in excessive deposition of lipids in hepatocytes. The effect of Jianpi Qinghua recipe on serum free fatty acid free fatty acidine FFAA and triglyceride triglyceride in liver of NAFLD rats was observed in order to study the effect of Jianpi Qinghua recipe on improving lipid metabolism and treating fatty liver by "invigorating spleen and clearing heat and removing dampness". Methods: fifty male Sprague-Dawley (SD) rats were selected, fed adaptively for one week, then randomly selected as normal group (n = 8), fed with basic feed and drinking tap water, the rest were treated with high fat group. Reference [1] was given a high-fat diet of 84% lard 5% egg yolk powder 1% cholesterol, drinking tap water for 10 weeks. Two rats were randomly selected from the model group and the normal group. The rats were anesthetized with chloral hydrate and killed, and the liver was taken for pathological section. Then the high fat group was divided into three groups: the model control group, the high and low dose group, the Dongbao Gantai group, the normal group and the model group. The rats were given normal saline for 8 weeks, except the normal group. The rats in each group were killed at the 18th week. The rats were killed by fasting overnight. After weighing their weight the next day, the rats were anesthetized by chloral hydrate intraperitoneally, and blood was collected from the abdominal aorta. Serum alanine aminotransferase (alanine aminotransferase) and syntax treedosum (ASTT) were measured, liver tissue was taken, weighed, liver homogenate and liver tissue sections were prepared, liver tissue FFA-TG content was detected and pathological changes of liver tissue were observed under light microscope. Results: the changes of FFA-TG content in liver tissue of rats in each group were determined. The results showed that the FFA-TG in the model group was significantly higher than that in the blank group (P 0.05), and the content of Jianpi Qinghua prescription was higher in the model group than that in the blank group. There was significant difference between the low dose group and the Dongbao Gantai group in reducing the level of FFAA TG in the liver of NAFLD rats compared with the model group (P 0.05), but there was no significant difference between the low dose group and the Dongbao Gantai group in the level of FFAF TG (P0.05), the high level of Jianpi Qinghua recipe, and the low dose group and the Dongbao Gantai group (P 0.05). Conclusion 1. Jianpi Qinghua prescription can reduce the concentration of FFA-TG in liver tissue of NAFLD model rats, and alleviate the degree of hepatocyte lipid change. 2. Jianpi Qinghua prescription can reduce the serum alt level of NAFLD model rats, which indicates that Jianpi Qinghua prescription has the effect of protecting liver and lowering enzyme.
【学位授予单位】：河南中医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R285.5

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