长期大强度运动对心室miR-155和miR-146a表达的影响

发布时间：2018-04-02 18:49

本文选题：大强度运动　切入点：心肌纤维化　出处：《体育科学》2017年07期

【摘要】：目的:研究长期大强度运动对心室miR-155和miR-146a表达的影响,探讨miR-155和miR-146a在调节运动性心肌纤维化和炎症中的作用。方法:48只8周龄雄性SD大鼠,随机分为对照组(Sed)和大强度运动组(IE),每组又分为8周、12周和16周,共6组,每组8只。对照组自由活动,大强度组以速度28 m/min,坡度10°的条件每天运动1 h,每周运动5天。最后一次运动后24 h后麻醉处死,迅速分离心脏,取左、右心室。采用RT-PCR法检测大鼠左、右心室miR-155和miR-146a及它们相应下游靶基因SOCS1和IRAK1 m RNA的表达水平。结果:长期大强度运动后,大鼠右心室miR-155表达水平显著升高,而左心室miR-155表达水平变化不大;16周大强度运动后,大鼠右心室miR-146a表达水平显著升高,而长期大强度运动对左心室miR-146a表达水平影响不大。长期大强度运动后,左、右心室SOCS1 m RNA的表达水平都没有发生变化;长期大强度运动后,右心室IRAK1 m RNA表达水平显著升高,而左心室IRAK1m RNA的表达水平变化不大。结论:长期大强度运动通过上调右心室miR-155的表达水平促进炎症反应是导致运动性右心室心肌纤维化的可能机制。
[Abstract]:Objective: To study the long-term effect of high intensity exercise on the expression of cardiac miR-155 and miR-146a, miR-155 and miR-146a in the regulation of exercise-induced myocardial fibrosis and inflammation in rats. Methods: 48 8 week old male SD rats were randomly divided into control group (Sed) and high intensity exercise group (IE), each group was divided for 8 weeks, 12 weeks and 16 weeks, a total of 6 groups, 8 rats in each group. The control group free activities, large strength group at a speed of 28 m/min, 10 degrees slope conditions every 1 h, exercise 5 days a week. The last 24 h after exercise after anesthesia were quickly separated from the heart, left. Right ventricular. RT-PCR method was used to detect the expression level of rat left and right ventricular miR-155 and miR-146a and their corresponding downstream target genes SOCS1 and IRAK1 m RNA. Results: after high intensity exercise, the rat right ventricular miR-155 expression level was significantly increased, while the left ventricular miR-155 expression levels did not change; 16 weeks of strength after exercise, big The expression level of rat right ventricular miR-146a increased significantly, while the long-term high-intensity exercise has little effect on the expression level of miR-146a in the left ventricle. The long-term after high intensity exercise, the left and right ventricular SOCS1 expression level of M RNA had no change; long after high intensity exercise, the expression level of IRAK1 M in right ventricular RNA were significantly increased, while the expression level changes the left ventricular IRAK1m RNA. Conclusion: the long-term high-intensity exercise through the up-regulated expression of miR-155 of the right ventricle inflammation is likely the mechanism of myocardial fibrosis of the right ventricle.

【作者单位】：上海体育学院运动科学学院;国家体育总局体育科学研究所;湖南工业大学体育学院;
【基金】：国家体育总局体育科学研究所基本科研业务费资助项目(16-21)
【分类号】：R87

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