替米沙坦对长期高糖高脂饮食诱导大鼠胰岛PTP-1B表达的影响

发布时间：2018-04-09 01:03

  本文选题：替米沙坦　切入点：高糖高脂　出处：《山西医科大学》2017年硕士论文

【摘要】：目的:1.观察替米沙坦对长期高糖高脂饮食诱导大鼠空腹血糖(FPG)、空腹胰岛素(FINS)、甘油三酯(TG)、稳态模型胰岛素抵抗指数(HOMA-IR)的影响。2.观察替米沙坦对长期高糖高脂饮食诱导大鼠胰岛蛋白酪氨酸磷酸酶1B(PTP-1B)表达的影响,探讨替米沙坦在改善胰岛素抵抗方面可能的机制。方法:购买雄性SD大鼠,6-8周龄,30只,体重(180-200g),适应性喂养1周,随机分为3组:对照组(n=10)、高糖高脂组(n=10)、替米沙坦组(n=10)。对照组普通饲料喂养,高糖高脂组和替米沙坦组高糖高脂喂养,24周末,替米沙坦组给予灌胃替米沙坦10mg/kg/d剂量(生理盐水溶解),干预12周。指标检测:替米沙坦干预12周后,大鼠禁食8—12小时,称重,腹腔注射乌拉坦麻醉,乌拉坦剂量4ml/kg,腹主动脉采血1ml,血标本经低速离心机离心3000r/min,20min,离取血浆,立即送检TG、FPG,葡萄糖氧化酶法测定FPG,全自动生化分析仪测定TG。另血浆0.5ml于-70℃冰箱保存,同期测定FINS,胰岛素ELISA试剂盒测定FINS。计算HOMA-IR=FPG×FINS/22.5。免疫组化分析:处死大鼠取血后放空血的方法,立即留取胰腺,把其置于10%中性福尔马林浓度溶液固定24—48小时,石蜡包埋,2um的厚度切片,行免疫组织化学染色,光学显微镜联合美国aperio公司scanscope数字病理扫描系统测定胰岛PTP-1B的含量。结果:1.替米沙坦干预12周后,与对照组相比,高糖高脂组FPG水平显著增高(P0.05)。与高糖高脂组相比,替米沙坦组FPG水平显著降低(P0.05)。2.替米沙坦干预12周后,与对照组相比,高糖高脂组血浆FINS水平显著增高(P0.05)。与高糖高脂组相比,替米沙坦组血浆FINS水平显著降低(P0.05)。3.替米沙坦干预12周后,与对照组相比,高糖高脂组血浆TG水平显著增高(P0.05)。与高糖高脂组相比,替米沙坦组血浆TG水平显著降低(P0.05)。4.替米沙坦干预12周后,与对照组相比,高糖高脂组HOMA-IR水平显著增高(P0.05)。与高糖高脂组相比,替米沙坦组HOMA-IR水平显著降低(P0.05)。5.替米沙坦干预12周后,与对照组相比,高糖高脂组PTP-1B含量显著升高(P0.05)。与高糖高脂组相比,替米沙坦组PTP-1B含量显著降低(P0.05)。结论:1.长期高糖高脂饮食诱导大鼠FPG、FINS、TG、HOMA-IR水平及胰岛PTP-1B表达水平增高。2.替米沙坦干预12周后FPG、FINS、TG、HOMA-IR水平及胰岛PTP-1B表达水平下降,推测替米沙坦改善胰岛素抵抗与降低胰岛PTP-1B蛋白表达有关。
[Abstract]:Purpose 1.To observe the effects of telmisartan on fasting blood glucose (FPG), fasting insulin (FINSN), triglyceride (TGN) and homeostasis index (HOMA-IRI) in rats induced by long-term high glucose and high fat diet.To observe the effect of telmisartan on the expression of islet protein tyrosine phosphatase (PTP-1B) in rats induced by high glucose and high fat diet for a long time, and to explore the possible mechanism of telmisartan in improving insulin resistance.Methods: 30 male Sprague-Dawley rats (6-8 weeks old) were randomly divided into three groups: control group (n = 10), high glucose and fat group (n = 10), telmisartan group (n = 10) and telmisartan group (n = 10).The control group was fed with common diet, the high-sugar and high-fat group and the telmisartan group were fed with high-sugar and high-fat diet for 24 weeks. The telmisartan group was given the dose of telmisartan 10mg/kg/d for 12 weeks.Index test: after telmisartan intervention for 12 weeks, rats fasted for 8 to 12 hours, weighing, intraperitoneal injection of uratan anesthetized, Uratan dose 4 ml / kg, abdominal aorta blood 1 ml, blood samples centrifuged by low speed centrifuge for 3 000 r / min for 20 min, plasma was extracted.FPG was detected by glucose oxidase method, and TGby automatic biochemistry analyzer.Plasma 0.5ml was stored at-70 鈩，

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