补肾固本方对去势大鼠骨形态计量学参数和相关生化指标影响的研究

发布时间：2018-04-09 11:09

本文选题：补肾固本方　切入点：骨质疏松　出处：《甘肃中医学院》2014年硕士论文

【摘要】：目的：通过检测去势大鼠经补肾固本方治疗后骨形态计量学指标和相关生化指标参数的变化评价补肾固本方对去势大鼠骨质疏松症模型的影响，探讨补肾固本方抗骨质疏松的作用机理。方法：选用SPF级wistar大鼠105只，随机分为空白组与造模组，造模组采用切除卵巢法制作大鼠骨质疏松症模型。造模成功后将造模组随机分为模型对照组，雌激素组，仙灵骨葆组，补肾固本方低、中、高剂量组；共分为7组，每组15只。术后一月按照试验设计剂量和剂型用不同药物分别给不同组别大鼠灌胃，每周灌胃6次，连续10周；并在大鼠处死前13天和前3天腹腔注射盐酸四环素荧光标记物。灌胃结束后，所有大鼠腹腔麻醉，采用股动脉取血法取血，取血6ml，静置2小时，2000r/min离心20分钟，取血清，，采用ELISA法检测雌激素、PINP、NTX的含量。取血后处死大鼠，并取出L2、右侧股骨制作不脱钙骨切片，测定骨形态计量学指标。结果：1、与空白组比较，模型对照组大鼠股骨上段骨组织和第二腰椎骨组织TBV%、Tb.Th、Tb.N、MAR显著降低（P0.01），雌激素、PINP显著降低（P0.01），NTX显著增高（P0.01）。 2、用药后，各治疗组大鼠股骨上段骨组织和第二腰椎骨组织TBV%、Tb.Th、Tb.N、MAR均明显增高（P0.05），雌激素、PINP明显升高（P0.05），NTX明显降低（P0.05）。 3、与雌激素组比较，补肾固本方高、中、低剂量组大鼠股骨上段骨组织和第二腰椎骨组织TBV%、Tb.Th、Tb.N、MAR均明显增高（P0.05），大鼠雌激素无明显差异，大鼠血清PINP均明显升高（P0.05）；补肾固本方中、低剂量组大鼠血清NTX明显增高（P0.05），补肾固本方高剂量组大鼠血清NTX无明显变化；。 4、与仙灵骨葆组比较，补肾固本方高、中、低剂量组大鼠股骨上段骨组织和第二腰椎骨组织TBV%，Tb.Th，Tb.N无明显差异，补肾固本方高、中、低剂量组大鼠第二腰椎骨组织MAR均明显降低（P0.05）；大鼠雌激素均无明显差异，补肾固本方高剂量组大鼠血清NTX明显降低（P0.05），补肾固本方中、低剂量组大鼠血清PINP均明显降低（P0.05）。 5、补肾固本方高、中、低剂量组各组之间比较，大鼠股骨上段骨组织和第二腰椎骨组织TBV%、Tb.Th、Tb.N、MAR均无明显差异，大鼠雌激素无明显差异；补肾固本方高剂量组较其它两组大鼠血清NTX明显降低（P0.05）。补肾固本方高剂量组较补肾固本方低剂量组大鼠血清PINP明显增高（P0.05）。结论：1、补肾固本方组方合理，可用于治疗临床最为常见的肾虚血瘀型骨质疏松症。 2、补肾固本方能明显提高去势骨质疏松症大鼠的骨量，这与抑制骨吸收，促进骨形成以及提高激素水平有关。 3、高剂量补肾固本方较中低剂量补肾固本方降低NTX水平的作用效果更为明显；高剂量补肾固本方较低剂量补肾固本方提高PINP水平的作用效果更加明显，可见高剂量补肾固本方治疗效果更为明显。 4、复方中药在骨质疏松症的治疗上效果明显，为今后的运用和进一步开发提供了理论依据。
[Abstract]:Objective: to evaluate the effect of Bushen Guben recipe on osteoporosis model of ovariectomized rats by detecting the changes of bone morphometry and related biochemical parameters after treatment with Bushen Guben recipe.Objective: to explore the mechanism of Bushen Guben prescription in anti-osteoporosis.Methods: 105 wistar rats of SPF grade were randomly divided into blank group and model group.After the model was successfully made, the model group was randomly divided into three groups: model control group, estrogen group, Xianling Gu Bao group, low, medium and high dosage group of Bushen Guben recipe, and were divided into 7 groups with 15 rats in each group.One month after the operation, different drugs were given to different groups of rats, 6 times a week for 10 weeks according to the designed dosage and dosage form, and tetracycline hydrochloride fluorescent label was injected intraperitoneally 13 days and 3 days before the rats were killed.All the rats were anesthetized intraperitoneally. Blood was collected by femoral artery and blood was taken from 6 ml. The rats were centrifuged for 20 minutes at 2 hours and 2000r / min for 20 minutes. The content of estrogen in PINPNTX was detected by ELISA method.The rats were killed after blood extraction, and L _ 2 was taken out. The right femur was made into undecalcified bone sections and bone morphometric indexes were measured.Results compared with the control group, the TBV bone tissue of the model control group and the second lumbar vertebrae bone tissue, TBV, TBV, TBV, TBV, TBV, TBV, TBV, TBV, TBV, TBV, TBV, TBV, TBV, TBV, TBV, TBV, TBV, TBV, TBV, TBV, TBV, TBV, TB@@2. After treatment, TBV in the proximal femur and the second lumbar vertebrae in each treatment group was significantly higher than that in the control group. The level of TBV in the upper femur and the second lumbar vertebrae was significantly higher than that in the control group (P < 0.05), and the level of estrogen PINP was significantly higher than that of the control group (P 0.05), and the level of estradiol was significantly lower than that of the control group (P 0.05).3Compared with estrogen group, Bushen Guben prescription was higher than that of estrogen group, middle and low dose group, TBV superior bone tissue and second lumbar vertebrae bone tissue TBV, Tb. Thine, Tb. Nomar were significantly higher than estrogen group, the serum PINP of rats were significantly increased P0. 05, Bushen Guben prescription, TBV, Tb. Tb. Nomar, TBV, TBV, TBV, TBV, TBV, TBV, TBV, TBV, Tb, Tb.The serum NTX of low dose group was significantly higher than that of Bushen Guben prescription group (P 0.05), but the serum NTX of Bushen Guben prescription group had no obvious change.4Compared with Xianling Gubao group, the Bushen Guben prescription was high, middle and low dose groups had no significant difference between the upper femur bone tissue and the second lumbar vertebrae bone tissue TBV.ThnTb.N, the Bushen Guben prescription was high and middle,5. There was no significant difference in TBV Tb. Tb. Tb. Nomar between the upper femur bone tissue and the second lumbar vertebrae bone tissue in the groups of high, middle and low doses of Bushen Guben recipe, and no significant difference in estrogen in rats.Compared with the other two groups, the serum NTX of the high dose group of Bushen Guben recipe was significantly lower than that of the other two groups.Compared with the low dose group, the serum PINP of the high dose group of Bushen Guben prescription was significantly higher than that of the low dose group of Bushen Guben recipe.Conclusion 1, the prescription of tonifying kidney and blood stasis is reasonable and can be used in the treatment of osteoporosis with kidney deficiency and blood stasis.2. Bushen Guben prescription can obviously increase the bone mass of ovariectomized rats, which is related to the inhibition of bone resorption, the promotion of bone formation and the increase of hormone level.3. The effect of high dose Bushen Guben recipe on reducing NTX level was more obvious than that of medium and low dose Bushen Guben recipe, and the effect of high dose Bushen Guben recipe on increasing PINP level was more obvious than that of low dose Bushen Guben recipe.It can be seen that the high-dose Bushen Guben prescription is more effective.4. The effect of compound Chinese medicine on the treatment of osteoporosis is obvious, which provides a theoretical basis for further application and further development.
【学位授予单位】：甘肃中医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R259;R285.5

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