异丙酚减轻氯胺酮麻醉对幼年大鼠海马神经元和认知功能的损害（英文）

发布时间：2018-04-11 09:00

本文选题：氯胺酮 + 异丙酚　；参考：《中国现代医学杂志》2015年06期

【摘要】：目的探讨异丙酚与氯胺酮复合麻醉对幼鼠发育中大脑认知功能的影响。方法 SD幼年大鼠被随机分为生理盐水组(NS组),氯胺酮组(K组);异丙酚组(P组),异丙酚+氯胺酮组(P+K组),每组20只,均腹腔注射相应药物1 ml,维持麻醉6 h。麻醉结束后各组幼鼠随机分为相同数量的A、B两部分,每部分10只。A部分于麻醉结束后取出幼鼠海马组织制作石蜡切片,进行海马CA1区神经元凋亡和蛋白质Bcl-2/Bax表达的分析;B部分于麻醉结束后3周行Morris水迷宫测试。结果 K组凋亡率较NS组明显增加(P0.05);P+K组凋亡率较P组明显上升(P0.05),但与K组相比,凋亡率明显减少(P0.05)。P+K组Bcl-2/Bax蛋白相对表达明显低于P组,但明显高于K组(P0.01)。Morris水迷宫结果显示K组学习记忆功能明显差于NS组(P0.05);P组及P+K组学习记忆功能优于K组(P0.05)。结论异丙酚复合氯胺酮麻醉,可明显减轻氯胺酮所致的幼年大鼠海马神经元的凋亡和大鼠远期认知功能的损害,异丙酚上调Bcl-2基因表达可能是其作用机制之一。
[Abstract]:Objective to investigate the effects of propofol and ketamine combined anesthesia on brain cognitive function in young rats.Methods Young SD rats were randomly divided into normal saline group (n = 20), ketamine group (n = 20), propofol group (n = 20), propofol group (n = 20) and propofol ketamine group (n = 20).After anesthesia, young rats in each group were randomly divided into two parts, the same number of Agna B, and 10 rats in each part were removed from the hippocampus after anesthesia to make paraffin sections.Apoptosis and protein Bcl-2/Bax expression in hippocampal CA1 neurons were analyzed. Morris water maze test was performed 3 weeks after anesthesia.Results the apoptotic rate of group K was significantly higher than that of group NS, but the relative expression of Bcl-2/Bax protein in group K was significantly lower than that in group P (P 0.05), but the relative expression of Bcl-2/Bax protein in group P (P 0.05) was significantly lower than that in group P (P < 0.05), but the expression of Bcl-2/Bax protein in group K was significantly lower than that in group P (P < 0.05).Conclusion propofol combined with ketamine anesthesia can significantly reduce the apoptosis of hippocampal neurons induced by ketamine and the long-term cognitive function in rats. The up-regulation of Bcl-2 gene expression by propofol may be one of its mechanisms.
【作者单位】：泸州医学院附属医院麻醉科;
【基金】：supported by Department of Health of Sichuan Province(No.:090205)
【分类号】：R726.1

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