恒河猴大脑中动脉M1段栓塞建模中麻醉方案的选择

发布时间：2018-04-14 10:05

本文选题：恒河猴 + 大脑中动脉栓塞　；参考：《中国比较医学杂志》2017年06期

【摘要】：目的为需要进行大脑中动脉M1段栓塞模型建立的恒河猴及其它大型实验动物提供一种简捷、迅速、安全的麻醉方法。方法采用健康成年恒河猴20只,均为雄性,年龄7~9岁,体重7~11 kg,基础麻醉应用0.1 m L/kg氯胺酮及速眠欣的混合液肌内注射,待动物入睡后行气管插管(ID:4.5~5.5#)。随后送入介入中心,入室后连接监护并建立静脉通路,完成有创动脉压及导尿管置入等。术中实验动物采用呼吸机控制呼吸,丙泊酚2~4 mg/kg/h持续泵入进行麻醉维持,术中根据动物的生命体征和肢体活动情况变化调整剂量,必要时追加上述氯胺酮与速眠新的混合液,并根据手术的需要调整心率、血压、体温等。溶栓前后行脑MRI造影,造影时停止输注麻醉药物,恢复动物自主呼吸。术中监测实验动物的心率、体温、有创动脉血压及血氧饱和度,并于麻醉诱导后和术中抽取动脉血行血气分析。结果共进行实验20次,均按预计方案实施完成,术中未发生动物躁动、呼吸抑制、心律失常等严重并发症,实验结束停药后,实验动物很快清醒并送回动物实验中心,进行后续处理。20只恒河猴15只存活24 h以上,5只死于溶栓后脑出血和大面积脑梗死面积较大。结论气管内插管全身麻醉合并复合麻醉维持为此类比较复杂的介入及MRI实验的顺利完成提供了一种安全、实用的麻醉方式。
[Abstract]:Objective to provide a simple, rapid and safe anesthetic method for rhesus monkey and other large laboratory animals which need to be embolized by M1 segment of middle cerebral artery.Methods Twenty healthy adult rhesus monkeys, aged 7 years and 9 years old, weighing 711 kg, were injected intramuscularly with 0.1 m L/kg ketamine and Shumianxin mixture solution. After the animals fell asleep, endotracheal intubation was performed.Then it was put into the intervention center, then connected with the monitoring and established the vein passage, completed the invasive arterial pressure and catheter implantation.During the operation, the experimental animals were controlled by ventilator, the propofol was continuously pumped in for anesthesia maintenance, the dosage was adjusted according to the vital signs of the animals and the changes of limb activity during the operation, and the mixture of ketamine and propofol was added when necessary.And adjust heart rate, blood pressure, body temperature and so on according to the need of operation.Cerebral MRI was performed before and after thrombolysis.The heart rate, body temperature, invasive arterial blood pressure and oxygen saturation of experimental animals were monitored during operation. Arterial blood was extracted for blood gas analysis after anesthesia induction and during operation.Results Twenty times of the experiment were carried out according to the expected plan. There were no serious complications such as restlessness, respiratory depression and arrhythmia during the operation. After the experiment was finished, the experimental animals soon woke up and sent back to the animal experimental center.After treatment, 15 rhesus monkeys survived for more than 24 hours and 5 died of intracerebral hemorrhage and large cerebral infarction after thrombolysis.Conclusion Intracheal intubation combined with general anesthesia combined with anesthesia can provide a safe and practical anesthetic method for the complicated interventional and the successful completion of MRI experiment.
【作者单位】：首都医科大学宣武医院麻醉科;
【基金】：北京市医院管理局“登峰计划”专项经费资助(编号DFL20150802) 国家自然科学基金项目(编号81500996) 中国博士后科学基金资助项目(编号2016T90114)
【分类号】：R-332;R614

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