单核细胞趋化因子-1在等长收缩运动促进大鼠缺血心肌侧支动脉生成中的作用

发布时间：2018-04-14 21:03

本文选题：等长收缩运动 + 心肌缺血　；参考：《中国康复医学杂志》2017年08期

【摘要】：目的:探讨单核细胞趋化因子-1(MCP-1)在等长收缩运动训练促进缺血心肌侧支动脉生成中的作用。方法:将造模成功的24只雄性SD大鼠,体重(200±20)g,随机分为4组:对照组(CG)、心肌缺血组(MI)、运动训练组(ET)、MCP-1抑制剂组(LG),每组均有6只大鼠。CG组连续2周皮下注射生理盐水;MI组连续2周皮下注射异丙肾上腺素(10mg/kg/d),造成心肌缺血;ET组连续2周皮下注射异丙肾上腺素并且进行等长收缩运动训练;LG组在ET组的基础上进行MCP-1抑制剂来氟米特灌胃。训练8周结束后,麻醉大鼠,取左心室心肌,采用微球法测定缺血区心肌相对侧支循环血流量(RCBF);采用免疫组化法测定缺血区心肌小动脉密度和单核细胞数量;以Western blot和荧光定量PCR法测定缺血心肌中MCP-1的蛋白及mRNA的表达。结果:ET组大鼠的相对侧支血流量(RCBF)、小动脉密度(AD)明显高于其余3组(P0.001),LG组的RCBF和AD与MI组相比无显著性差异;ET组大鼠缺血心肌中单核细胞数量、MCP-1 mRNA以及MCP-1蛋白表达均显著高于其余各组(P0.001),而LG组大鼠缺血心肌中单核细胞数量、MCP-1 mRNA以及MCP-1蛋白表达与MI组相比无显著性差异。结论:持续8周的等长收缩运动训练可以增加大鼠缺血心肌中MCP-1的表达,从而促进缺血心肌侧支动脉的生成。
[Abstract]:Aim: to investigate the role of monocyte chemokine-1 (MCP-1) in the promotion of collateral artery formation by isometric contractile exercise.Methods: 24 male SD rats were successfully established.The rats were randomly divided into four groups: control group, myocardial ischemia group and exercise training group. Six rats in each group were injected with isoproterenol 10 mg / kg / kg / d for 2 consecutive weeks, and 6 rats in each group were subcutaneously injected with normal saline for 2 weeks, and the control group was subcutaneously injected with isoproterenol (10 mg / kg / d) for 2 weeks, and the rats in exercise training group were given isoproterenol 10 mg / kg / d subcutaneously for 2 weeks.Isoprenaline was injected subcutaneously for 2 weeks and isometric exercise training was performed in the LG group. The LG group was given MCP-1 inhibitor leflunomide on the basis of et group.After 8 weeks of training, the left ventricular myocardium was taken from anesthetized rats, the relative collateral circulation blood flow (RCBFN) was measured by microsphere method, the density of myocardial arterioles and the number of monocytes in ischemic myocardium were measured by immunohistochemical method.Western blot and fluorescence quantitative PCR were used to detect the expression of MCP-1 and mRNA in ischemic myocardium.Results compared with MI group, the relative collateral blood flow (RCBFN) and arteriolar density (ADD) of the rats in the control group were significantly higher than those in the other three groups. There was no significant difference in RCBF and AD between the two groups. The expression of MCP-1 mRNA and MCP-1 protein in ischemic myocardium of rats in the et group was similar to that in the MI group.The expression of MCP-1 mRNA and MCP-1 protein in ischemic myocardium of LG group was not significantly different from that of MI group.Conclusion: the isometric contractile exercise training for 8 weeks can increase the expression of MCP-1 in ischemic myocardium and promote the formation of collateral arteries in ischemic myocardium.
【作者单位】：南京医科大学第一附属医院;
【基金】：国家自然科学基金资助项目(81472164)
【分类号】：R541.4

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