翻白草对大鼠溃疡性结肠炎的药效及作用机制研究

发布时间：2018-04-15 21:29

本文选题：翻白草 + 溃疡性结肠炎　；参考：《中国中医基础医学杂志》2017年09期

【摘要】：目的:研究翻白草对大鼠溃疡性结肠炎的药效作用,并探讨翻白草治疗UC的可能分子机制。方法:采用三硝基苯磺酸-乙醇灌肠法复制溃疡性结肠炎(UC)大鼠模型,实验分为空白对照组、模型对照组、柳氮磺吡啶组、翻白草低、中、高剂量组6组。连续给药14 d后水合氯醛麻醉,取病变结肠组织石蜡包埋、切片、染色后镜下观察,剩余结肠组织采用western blot方法分别检测TLR4及NF-κBP65。结果:DAI指数及病理形态变化表明,与空白组比较模型组差异有统计学意义,翻白草低剂量组治疗效果最佳;与空白组比较模型组大鼠结肠组织NF-κBP65表达水平显著增加,翻白草给药组均能下调NF-κBP65的表达水平。结论:翻白草能通过调控NF-κBP65表达水平以发挥对UC的治愈作用。
[Abstract]:Aim: to study the pharmacodynamic effect of Leucaria verticillata on ulcerative colitis in rats and to explore the possible molecular mechanism of the treatment of UC.Methods: the rat model of ulcerative colitis was established by trinitrobenzenesulfonic acid ethanol enema. The rats were divided into six groups: blank control group, model control group, salicylazosulfopyridine group, low, middle and high dose groups.After 14 days of continuous administration, chloral hydrate was used to anesthetize the lesion colon tissue, then paraffin embedded and sectioned. The residual colonic tissue was examined for TLR4 and NF- 魏 BP65by western blot method.Results the changes of the weight Dai index and pathological morphology showed that there was a significant difference between the model group and the blank group, and the treatment effect of the low dose group was the best, and the expression of NF- 魏 BP65 in the colon tissue of the model group was significantly higher than that of the blank group.The expression of NF- 魏 BP65 was down-regulated in both groups.Conclusion: Herba Leymus can play a therapeutic role in UC by regulating the expression of NF- 魏 BP65.
【作者单位】：中央民族大学中国少数民族传统医学研究院;
【基金】：中央高校基本科研业务费资助项目(2015MDQN15)-基于肠黏膜屏障损伤修复的彝药翻白草抗UC药效评价及作用机制研究
【分类号】：R285.5

【相似文献】