毒扁豆碱对异氟醚麻醉后大鼠海马炎性反应、caspase-3表达及认知功能的影响

发布时间：2018-04-17 11:48

本文选题：毒扁豆碱 + 异氟醚　；参考：《广东医学》2015年03期

【摘要】：目的探讨毒扁豆碱对异氟醚麻醉后大鼠海马炎性反应、caspase-3表达及认知功能的影响。方法2月龄SD大鼠80只,体重280~320 g,分为4组(n=20):C组吸入含有30%氧气的空氧混合气体6 h;I组吸入1.4%的异氟醚6 h;PI组与P组腹腔注射毒扁豆碱100μg/kg,每8 h 1次,共3次,首次给药后,PI组吸入1.4%的异氟醚6 h,P组吸入同浓度载气6 h。麻醉结束行动脉血气分析,12 h后取海马组织ELISA法检测TNF-α、IL-1β、IL-6含量,RT-PCR法检测caspase-3 m RNA的表达。光镜下观察海马CA1区锥体细胞形态学变化;麻醉后第2~7天评估认知功能。结果 4组间血气水平差异无统计学意义(P0.05)。与C、P组比较,I、PI组IL-1β、IL-6含量及caspase-3 m RNA水平升高,逃避潜伏期延长,探索时间缩短(P0.05),海马CA1区锥体细胞凋亡增多;与I组比较,PI组IL-1β、IL-6含量及caspase-3 m RNA表达降低(P0.05),逃避潜伏期缩短,探索时间延长(P0.05),CA1区锥体细胞凋亡减少。P组与C组比较差异无统计学意义(P0.05)。TNF-α在4组间差异无统计学意义(P0.05)。结论毒扁豆碱可改善异氟醚麻醉引起的大鼠认知功能损害,其机制可能与抑制海马炎性反应及神经元凋亡有关。
[Abstract]:Objective to investigate the effect of lentil on the expression of caspase-3 and cognitive function of hippocampal inflammatory response after isoflurane anesthesia in rats.Methods 80 2-month-old SD rats, weighing 280 ~ 320 g, were divided into 4 groups. The rats in group I inhaled 1.4% isoflurane for 6 h, Pi group and group P were injected with 100 渭 g / kg of lentil alkaloid once every 8 hours for 3 times.After the first administration, the Pi group inhaled 1.4% isoflurane for 6 h and the P group inhaled the same concentration of carrier gas for 6 h.After 12 hours of arterial blood gas analysis at the end of anesthesia, the content of TNF- 伪, IL-1 尾 and IL-6 in hippocampal tissue was detected by ELISA method. RT-PCR was used to detect the expression of caspase-3 m RNA.The morphological changes of pyramidal cells in hippocampal CA1 region were observed under light microscope, and cognitive function was evaluated 7 days after anesthesia.Results there was no significant difference in blood gas level among the four groups (P 0.05).Compared with the control group, the content of IL-1 尾 -IL-6 and the level of caspase-3 m RNA were increased, the latency of escape was prolonged, the exploring time was shortened (P 0.05), the apoptosis of pyramidal cells in the CA1 region of hippocampus was increased, the content of IL-1 尾 -IL-6 and the expression of caspase-3 m RNA in the Pi group were significantly lower than those in the control group (P 0.05), and the escape latency was shortened.There was no significant difference in pyramidal cell apoptosis between group P and group C. There was no significant difference in P0.05N. TNF- 伪 between the four groups.Conclusion lentil can improve the cognitive impairment induced by isoflurane in rats, and its mechanism may be related to the inhibition of hippocampal inflammatory response and neuronal apoptosis.
【作者单位】：兰州大学第一医院麻醉科;
【分类号】：R285.5

【参考文献】