饮食诱导肥胖大鼠瘦素与血管内皮功能的研究

发布时间：2018-04-17 17:13

本文选题：饮食诱导肥胖 + 瘦素　；参考：《天津医科大学》2014年硕士论文

【摘要】：目的通过建立饮食诱导肥胖(DIO)大鼠模型,观察肥胖模型大鼠的血浆瘦素等生化指标的变化,测量血压并进行血流动力学研究,观察血压改变与内皮功能的关系,探讨瘦素对肥胖大鼠血压影响的机制。方法 1.选用SD雄性大鼠40只完全随机分组,对照组(A组)10只喂以普通饲料,肥胖组30只喂以高脂饲料,根据大鼠体重及肥胖评定指数(Lee's指数)判定肥胖模型是否成功。造模成功后随机分为B、C、D三组各10只并且C、D两组再分别高脂饲养2周及4周。 2.血清学检测：造模成功后,分别于10周末、12周末及14周末大鼠麻醉动脉采血作血液生化检查,采用酶法测定甘油三酯(TG)、胆固醇(TC)、葡萄糖(GLU)、高密度脂蛋白(HDL-C)水平。应用酶法测定一氧化氮(NO)、总一氧化氮合酶(TNOS)、诱导型一氧化氮合酶(iNOS)、内皮型一氧化氮合酶(eNOS).超氧阴离子、超氧化物歧化酶(SOD)水平,应用酶联免疫吸附法检测瘦素的水平。 3.血压测定：不同组大鼠观察期末将大鼠麻醉后分离暴露左颈静脉及右颈动脉,然后经左颈静脉分别匀速推注乙酰胆碱和硝普钠各5分钟,推注速度为50μg/kg·min,同时经右颈动脉插管,EPS电生理仪描记收缩压演变过程。结果 1.与对照组相比,饲养10周后肥胖组大鼠的体重较对照组显著增加(P0.01), Lee's指数明显增加(P0.05),以体重比正常组平均体重增加10%及Lee's指数比正常组平均水平增加1.5%作为判断肥胖大鼠的标准,提示造模成功。 2.饮食诱导肥胖大鼠随着饲养周期的增加,瘦素水平较对照组显著增加(P0.01)。 3.与对照组对比,肥胖组随着饲养周期的延长,NO、NOS及SOD血清水平均降低(P0.05), TNOS及iNOS及均升高(P0.05),而超氧阴离子水平没有明显变化(P0.05)。 4.颈动脉插管直接测血压显示：肥胖各组大鼠基础收缩压明显高于对照组(P0.05),颈静脉推注乙酰胆碱和硝普钠,各组大鼠血压水平明显下降,对照组推注乙酰胆碱(P0.01)及硝普钠(P0.05)引起降压幅度高于肥胖组,颈静脉推注乙酰胆碱和硝普钠比较显示,对照组间降压幅度无统计学意义(P0.05),各个肥胖组间,硝普钠降压幅度高于乙酰胆碱P0.05),对照组间降压幅度无统计学意义(P0.05)。
[Abstract]:PurposeThe changes of plasma leptin and other biochemical indexes were observed, blood pressure was measured and hemodynamics was studied, and the relationship between changes of blood pressure and endothelial function was observed by establishing diet-induced adipose-induced diol (Dio) rat model.To investigate the mechanism of leptin on blood pressure in obese rats.Method1.Forty Sprague-Dawley male rats were randomly divided into control group (n = 10) and obese group (n = 30).The rats were randomly divided into three groups (n = 10 each) and fed with hyperlipidemia for 2 weeks and 4 weeks respectively.2.Serological examination: after successful modeling, blood samples were collected from anesthetized arteries of rats at the end of 10 weeks, 12 weeks and 14 weeks, respectively. The levels of triglyceride triglyceride (TGN), cholesterol triglyceride (TCN), glucose glutin (GLU) and high density lipoprotein (HDL-C) were determined by enzymatic method.Total nitric oxide synthase (TNOS), inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (Enos) were determined by enzyme method.The levels of superoxide anion and superoxide dismutase (SOD) were determined by enzyme linked immunosorbent assay (Elisa).3.Blood pressure measurement: the left jugular vein and right carotid artery were isolated and exposed after anaesthesia in different groups of rats at the end of the observation period. Acetylcholine and sodium nitroprusside were injected through the left jugular vein for 5 minutes, respectively.The injection rate was 50 渭 g/kg, and the evolution of systolic blood pressure was recorded by right carotid artery intubation with EPS electrophysiological instrument.Result1.Compared with the control group,After 10 weeks of feeding, the body weight of obese rats was significantly higher than that of the control group, and the Lee's index was significantly higher than that of the control group. The average body weight of the obese rats was increased by 10% compared with the normal group and the Lee's index was 1.5% higher than the average level of the normal group.The results suggest that the model is successful.2.The level of leptin in obese rats induced by diet was significantly higher than that in the control group with the increase of feeding cycle.3.Compared with the control group, the serum levels of nitric oxide synthase (NOS) and SOD in obese group were decreased with the increase of feeding period, while the levels of TNOS and iNOS were increased, while the level of superoxide anion did not change significantly.4.Direct blood pressure measurement by carotid artery intubation showed that the basic systolic blood pressure of obese rats was significantly higher than that of the control group (P 0.05). The blood pressure of rats in each group was significantly decreased by injecting acetylcholine and sodium nitroprusside into the jugular vein.The hypotension amplitude of the control group was higher than that of the obese group. The comparison between the jugular vein injection of acetylcholine and sodium nitroprusside showed that there was no significant difference between the control group and the control group (P 0.05).Sodium nitroprusside was higher than acetylcholine P0. 05% in lowering blood pressure, but there was no significant difference between control group (P 0. 05 and P 0. 05).
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R589.2

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